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cytological and Proteomic analysis of Ginkgo biloba Pollen Intine

Horticultural Plant Journal 2020年第4期6卷 257-266页

作者： Weixing Li Yunling Ye Fangmei Cheng Yan Lu Biao Jin Li Wang College of Horticulture and Plant Protection Yangzhou UniversityYangzhou 225009China Suqian Academy of Protected Horticulture Nanjing Agricultural UniversitySuqianJiangsu 223800China Instrumental analysis Center Yangzhou UniversityYangzhou 225009China

The pollen intine plays important roles in pollen germination and tube growth,but related information in Ginkgo biloba remains *** isolated and obtained de-exined pollen from *** fluorescent probes,we observed the strongest cellulose fluorescence in the pollen ***-esterified pectin immunolabeled with JIM5 was present throughout the entire cell wall,whereas esterified pectin recognized by the monoclonal antibody JIM7 was concentrated in some *** staining with aniline blue was observed across the entire surface of the pollen *** results were confirmed by Fourier Transform InfraRed(FTIR)*** also used proteomic approaches to identify different proteins between mature and de-exined pollen(48h after hydration)in *** on mass spectrometry,de-exined pollen had more proteins than mature pollen,including calmodulin,serine hydroxymethyltransferase,β-galactosidase 6,and class IV *** to Gene Ontology(GO)analysis,the differentially expressed proteins were mainly associated with transportation,defense reaction,sugar metabolism,energy metabolism,signal transduction,and cell wall *** findings suggest that most proteins involved in pollen germination and pollen tube growth are synthesized during pollen hydration,indicating the important role of pollen hydration in the reproductive process of ***.

关键词： Ginkgo biloba pollen intine cytological analysis proteomics mass spectrometry

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Retinoblastoma in a young adult mimicking Coats'disease

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International Journal of Ophthalmology(English edition) 2012年第5期5卷 625-629页

作者： Zheng Zhang, Ji-Tong Shi, Ning-Li Wang, Jian-Min Ma Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Vision Science Key Lab, Beijing 100730, China Beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University Beijing Ophthalmology & Vision Science Key Lab Beijing 100730 China.

Retinoblastoma is the most common childhood primary intraocular malignancy, with the majority of cases being diagnosed before 5 years of age. Retinoblastoma in adults is extremely rare. Here, we report the case of a 20-year-old man who presented with a 3 year history of blurred vision in the right eye. Imaging did not reveal the typical presentation of retinoblastoma. After considering Coats’ disease, a diagnosis of late-presenting retinoblastoma was made through cytological analysis. Diagnosis of retinoblastoma should be considered in the presence of uncertain mass lesions in the fundus of an adult.

关键词： adult retinoblastoma Coats'disease cytological analysis

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