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Acta Mechanica Sinica 2011年第6期27卷 1081-1090页

作者： Li Wang Mei-Wen An Xiao-Na Li Fang Yang Yang Liu Institute of Applied Mechanics and Biomedical Engineering Taiyuan University of Technology 030024 Taiyuan China

To probe the contributions of polar cortical cytoskeleton and the surface tension of daughter cells to intercellular bridge thinning dynamics during cytokinesis,we applied cytochalasin D（CD） or colchicine（COLC） in a highly localized manner to polar regions of dividing normal rat kidney（NRK） *** observed cellular morphological changes and analyzed the intercellular bridge thinning trajectories of dividing cells with different polar cortical *** blebbistatin（BS） application was used to obtain cells losing active contractile force *** results show that locally released CD or colchicine at the polar region caused inhibition of cytokinesis before *** treatment at phases after ingression allowed completion of cytokinesis but dramatically influenced the trajectories of intercellular bridge *** single polar cortical actin induced transformation of the intercellular bridge thinning process,and polar cortical tension controlled deformation time of intercellular *** study provides a feasible framework to induce and analyze the effects of local changes in mechanical properties of cellular components on single cellular cytokinesis.

关键词： cytokinesis Normal rat kidney epithelial cells Intercellular bridge Polar cortical cytoskeleton - Cortical surface tension

AtMAC stabilizes the phragmoplast by crosslinking microtubules and actin filaments during cytokinesis

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Journal of Integrative Plant Biology 2023年第8期65卷 1950-1965页

作者： Pingzhou Du Yu Liu Lu Deng Dong Qian Xiuhua Xue Ting Yang Tonghui Li Yun Xiang Haiyun Ren Center for Biological Science and Technology Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of EducationZhuhai-Macao Biotechnology Joint LaboratoryAdvanced Institute of Natural ScienceBeijing Normal UniversityZhuhai 519087China MOE Key Laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou UniversityLanzhou 73000oChina

The phragmoplast,a structure crucial for the completion of cytokinesis in plant cells,is composed of antiparallel microtubules(MTs)and actin filaments(AFs).However,how the parallel structure of phragmoplast MTs and AFs is maintained,especially during centrifugal phragmoplast expansion,remains ***,we analyzed a new Arabidopsis thaliana MT and AF crosslinking protein(AtMAC).When AtMAC was deleted,the phragmoplast showed disintegrity during centrifugal expansion,and the resulting phragmoplast fragmentation led to incomplete cell *** of AtMAC increased the resistance of phragmoplasts to depolymerization and caused the formation of additional phragmoplasts during *** experiments showed that AtMAC crosslinked MTs and AFs in vitro,and the truncated AtMAC protein,N-CC1,was the key domain controlling the ability of *** analysis showed that N-CC1(51–154)is the key domain for binding MTs,and N-CC1(51–125)for binding *** conclusion,AtMAC is the novel MT and AF crosslinking protein found to be involved in regulation of phragmoplast organization during centrifugal phragmoplast expansion,which is required for complete cytokinesis.

关键词： actin filaments AtMAC cytokinesis phragmoplast microtubules

NudCL2 is required for cytokinesis by stabilizing RCC2 with Hsp90 at the midbody

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Protein & Cell 2024年第10期15卷 766-782页

作者： Xiaoyang Xu Yuliang Huang Feng Yang Xiaoxia Sun Rijin Lin Jiaxing Feng Mingyang Yang Jiaqi Shao Xiaoqi Liu Tianhua Zhou Shanshan Xie Yuehong Yang Department of Cell Biology Institute of Gastroenterology of the Second Affliated HospitalZhejiang University School of MedicineHangzhou 310009China Research Center for Children's Health and Innovation Binjiang Institute of Zhejiang UniversityHangzhou 310053China Department of Biochemistry Purdue UniversityWest LafayetteIN 47907United States Cancer Center Zhejiang UniversityHangzhou 310058China Center for RNA Medicine International Institutes of Medicinethe Fourth Affliated Hospital of Zhejiang University School of MedicineYiwu 322000China Children's Hospital Zhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China

cytokinesis is required for faithful division of cytoplasmic components and duplicated nuclei into two daughter ***,a protein-dense organelle that forms at the intercellular bridge,is indispensable for success-ful ***,the regulatory mechanism of cytokinesis at the midbody still remains ***,we unveil a critical role for NudC-like protein 2(NudCL2),a co-chaperone of heat shock protein 90(Hsp90),in cytokinesis regulation by stabilizing regulator of chromosome condensation 2(RCC2)at the midbody in mam-malian ***2 localizes at the midbody,and its downregulation results in cytokinesis failure,multinu-cleation,and midbody *** iTRAQ-based quantitative proteomic analysis,we find that RCC2 levels are decreased in NudCL2 knockout(KO)***,Hsp90 forms a complex with NudCL2 to stabilize RCC2,which is essential for ***2 depletion mirrors phenotypes observed in NudCL2-downregulated ***,ectopic expression of RCC2 rescues the cytokinesis defects induced by NudCL2 deletion,but not vice ***,our data reveal the significance of the NudCL2/Hsp90/RCC2 pathway in cytokinesis at the midbody.

关键词： cytokinesis midbody NudCL2 Hsp90 RCC2

The formation of Ca^(2+) gradients at the cleavage furrows during cytokinesis of Zebrafish embryos

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Frontiers in Biology 2010年第4期5卷 369-377页

作者： Yun-Bo GUO Ya WEN Wen-Xue GAO Jing-Chao LI Peng ZHOU Zai-Ling BAI Bo ZHANG Shi-Qiang WANG State Key Laboratory of Biomembrane and Membrane Biotechnology and Center of Developmental Biology and GeneticsCollege of Life SciencesPeking UniversityBeijing 100871China

In dividing embryos,a localized elevation in intracellular Ca^(2+)([Ca^(2+)]i)at the cleavage furrow has been shown to be essential for ***,the underlying mechanisms for generating and maintaining these[Ca^(2+)]_(i) gradients throughout cytokinesis are not fully *** the present study,we analyzed the role of inositol 1,4,5-trisphosphate receptors(IP3Rs)and endoplasmic reticulum(ER)distribution in determining the intracellular Ca^(2+) gradients in early zebrafish *** of the injected Ca^(2+) indicator,Indo-1,showed that during the first cell division a standing Ca^(2+) gradient was formed~35 min after fertilization,with the[Ca^(2+)]_(i) spatially decaying from 500–600 nmol/L at the cleavage furrow to 100–200 nmol/L around the *** the IP3R immunohistochemical fluorescence was relatively concentrated in the peri-furrow region,ER labeling was relatively enriched in both peri-furrow and peri-nuclear *** simulation suggested that a divergence in the spatial distribution of IP3R and the locations of Ca^(2+) uptake within the ER was essential for the formation of a standing Ca^(2+) gradient,and the Ca^(2+) gradient could only be well-established under an optimal stoichiometry of Ca^(2+) uptake and ***,while inhibition of IP3R Ca^(2+) release blocked the generation of the Ca^(2+)gradient at a lower[Ca^(2+)]_(i) level,both Ca^(2+) release stimulation by inositol 1,4,5-trisphosphate(IP3)injection and ER Ca^(2+) pump inhibition by cyclopiazonic acid also eliminated the Ca^(2+) gradients at higher[Ca^(2+)]_(i) *** results suggest a dynamic relationship between ER-mediated Ca^(2+) release and uptake that underlies the maintenance of the perifurrow Ca^(2+) gradient and is essential for cytokinesis of zebrafish embryos.

关键词： Ca^(2+)gradients cytokinesis zebrafish

cytokinesis and cancer:Polo loves ROCK'n' Rho(A)

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Journal of Genetics and Genomics 2010年第3期37卷 159-172页

作者： Jing Li Jue Wang Hong Jiao Ji Liao Xingzhi Xu Laboratory of Cancer Biology College of Life Science Capital Normal University Beijing 100048 China

cytokinesis is the last step of the M （mitosis） phase, yet it is crucial for the faithful division of one cell into two. cytokinesis failure is often associated with cancer. Cytoldnesis can be morphologically divided into four steps： cleavage furrow initiation, cleavage furrow ingression, midbody formation and abscission. Molecular studies have revealed that RhoA as well as its regulators and effectors are im- portant players to ensure a successful cytokinesis. At the same time, Polo-like kinase 1 （Plkl） is an important kinase that can target many substrates and carry out different functions during mitosis, including cytokinesis. Recent studies are beginning to unveil a closer tie be- tween Plkl and RhoA networks. More specifically, Plkl phosphorylates the centralspindlin complex Cyk4 and MKLPI/CHO1, thus re- cruiting RhoA guanine nucleotide-exchange factor （GEF） Ect2 through its phosphopeptide-binding BRCT domains. Ect2 itself can be phosphorylated by Plkl in vitro. Plkl can also phosphorylate another GEF MyoGEF to regulate RhoA activity. Once activated, RhoA-GTP will activate downstream effectors, including ROCK1 and ROCK2. ROCK2 is among the proteins that associate with Plkl Polo-binding domain （PBD） in a large proteomic screen, and Plkl can phosphorylate ROCK2 in vitro. We review current understandings of the interplay between Plkl, RhoA proteins and other proteins （e.g., NudC, MKLP2, PRC 1, CEP55） involved in cytokinesis, with particular emphasis of its clinical implications in cancer.

关键词： Polo-like kinase 1 RhoA GTPase Rho kinase cytokinesis

Membrane Phospholipid Redistribution in cytokinesis:A Theoretical Model

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Acta Biochimica et Biophysica Sinica 2005年第9期37卷 643-648页

作者： Mei-Wen AN Wen-Zhou WU Wei-Yi CHEN Institute of Applied Mechanics,Taiyuan University of Technology,Taiyuan 030024,China InstituteofAppliedMechanics TaiyuanUniversityofTechnologyTaiyuan030024China

In cell mitosis,cytokinesis is a major deformation process,during which the site of thecontractile ring is determined by the biochemical stimulus from asters of the mitotic apparatus,actin andmyosin assembly is related to the motion of membrane phospholipids,and local distribution and arrangementof the microfilament cytoskeleton are different at different cytokinesis *** on the Zinemanas-Nirmodel,a new model is proposed in this study to simulate the entire process by coupling the biochemicalstimulus with the mechanical *** were three assumptions in this model:the movements ofphospholipid proteins are driven by gradients of biochemical stimulus on the membrane surface;the localassembly of actin and myosin filament depends on the amount of phospholipid proteins at the same location;and the surface tension includes membrane tensions due to both the passive deformation of the membraneand the active contraction of actin filament,which is determined by microfilament redistribution *** model could explain the dynamic movement of microfilaments during cytokinesis andpredict cell *** calculated results from this model demonstrated that the reorientation ofphospholipid proteins and the redistribution and reorientation of microfilaments may play a crucial role in *** model may better represent the cytokinesis process by the introduction of biochemical stimulus.

关键词： cytokinesis biochemical stimulus phospholipid actomyosin microfilament

The Membrane-Associated Sec1/Munc18 KEULE is Required for Phragmoplast Microtubule Reorganization During cytokinesis in Arabidopsis

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Molecular Plant 2016年第4期9卷 528-540页

作者： Alexander Steiner Lin Muller Katarzyna Rybak Vera Vodermaier Eva Facher Martha Thellmann Raksha Ravikumar Gerhard Wanner Marie-Theres Hauser Farhah F. Assaad Botany Department School of Life Sciences Technische Universitat Munchen Emil-Ramann-Street 4 85354 Freising Germany Department Biologie I Ludwig-Maximillians Universitat 82152 Planegg-Martinsried Germany Department of Applied Genetics and Cell Biology University of Natural Resources and Life Sciences 1190 Vienna Austria

cytokinesis, the partitioning of the cytoplasm following nuclear division, requires extensive coordination between membrane trafficking and cytoskeletal dynamics. In plants, the onset of cytokinesis is characterized by the assembly of a bipolar microtubule array, the phragmoplast, and of a transient membrane compartment, the cell plate. Little is known about the coordination between membrane deposition at the cell plate and the dynamics of phragmoplast microtubules. In this study, we monitor the localization dynamics of microtubule and membrane markers throughout cytokinesis. Our spatiotemporal resolution is consistent with the general view that microtubule dynamics drive membrane movements. Nonetheless, we provide evidence for active sorting at the cell plate and show that this is, at least in part, mediated by the TRAPPII tethering complex. We also characterize phragmoplast microtubule organization and cell plate formation in a suite of cytokinesis-defective mutants. Of four mutant lines with defects in phragmoplast microtubule organization, only morl microtubule-associated mutants exhibited aberrant cell plates. Conversely, the mutants with the strongest impairment in phragmoplast microtubule reorganization are keule alleles, which have a primary defect in membrane fusion. Our findings identify the SEC1/Munc18 protein KEULE as a central regulatory node in the coordination of membrane and microtubule dynamics during plant cytokinesis.

关键词： cytokinesis cell plate phragmoplast microtubule KEULE KNOLLE PLEIADE/MAP65-3

Regulation of cytokinesis by Exocyst Subunit SEC6 and KEULE in Arabidopsis thaliana

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Molecular Plant 2013年第6期6卷 1863-1876页

作者： Jiandong Wu Xiaoyun Tan ChengyunWu Kun Cao Yiqun Bao College of Life Sciences Nanjing Agricultural University Nanjing 210095 People's Republic of China

Proper vesicle tethering and membrane fusion at the cell plate are essential for cytokinesis. Both the vesicle tethering complex exocyst and membrane fusion regulator KEULE were shown to function in cell plate formation, but the exact mechanisms still remain to be explored. In this study, using yeast two-hybrid （Y-2-H） assay, we found that SEC6 interacted with KEULE, and that a small portion of C-terminal region of KEULE was required for the interaction. The direct SEC6-KEULE interaction was supported by further studies using in vitro pull-down assay, immunoprecipitation, and in vivo bimolecular florescence complementation （BIFC） microscopy, sec6 mutants were male gametophytic lethal as reported; however, pollen-rescued sec6 mutants （PRsec6） displayed cytokinesis defects in the embryonic cells and later in the leaf pavement cells and the guard cells. SEC6 and KEULE proteins were co-localized to the cell plate during cytokine- sis in transgenic Arabidopsis. Furthermore, only SEC6 but not other exocyst subunits located in the cell plate interacted with KEULE in vitro. These results demonstrated that, like KEULE, SEC6 plays a physiological role in cytokinesis, and the SEC6-KEULE interaction may serve as a novel molecular linkage between arriving vesicles and membrane fusion machin- ery or directly regulate membrane fusion during cell plate formation in plants.

关键词： SEC6 KEULE cytokinesis vesicle tethering membrane fusion embryogenesis plant development.

Calmodulin regulates the post-anaphase reposition of centrioles during cytokinesis

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Cell Research 2005年第7期15卷 548-552页

作者： Yue Yue YU, Gu DAI, Fei Yan PAN, Jie CHEN, Chao Jun LI*Jiangsu Key Laboratory for Molecular & Medical Biotechnology, School of Life Sciences, Nanjing Normal University.Nanjing 210097, China JiangsuKeyLaboratoryforMolecular&MedicalBiotechnology SchoolofLifeSciencesNanjingNormalUniversityNanjing210097China

A transient postanaphase repositioning of the centriole is found to control the completion of cytokinesis. Using agreen fluorescent protein-calmodulin fusion protein as a living cell probe, we have previously found that calmodulin isassociated with the initiation and progression of cytokinesis. In this study, we further studied the effect of calmodulinon the repositioning of the centriole and subsequent cell cycle progression. When activity of calmodulin is inhibited, theregression of the centriole from the intercellular bridge to the cell center is blocked, and thus the completion of celldivision is repressed and two daughter cells are linked by longer cell bridge in perturbed cells. W7 treatment duringcytokinesis also results in unfinished cytokinesis and stopped G1 phase. These results suggest that calmodulin activity isrequired for centriole repositioning and can affect the completion of cytokinesis and cell cycle progression.

关键词： CaM centriole reposition cytokinesis W7.

The septins FaCdc3 and FaCdc12 required for septum formation and cytokinesis,affect lipid metabolism,asexual and sexual development in Fusarium asiaticum

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The septins FaCdc3 and FaCdc12 required for septum formation...