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Value of cystatin C in predicting atrial fibrillation recurrence after radiofrequency catheter ablation

Journal of Geriatric Cardiology 2018年第12期15卷 725-731页

作者： Li-Li JIN Ling YOU Rui-Qin XIE Department of Cardiology the Second Hospital of Hebei Medical UniversityShijiazhuangChina

Backgroud Recent studies have demonstrated that cystatin C is a valuable risk marker for cardiovascular disease morbidity and mortality.Therefore,we hypothesized that the pre-ablation cystatin C level was associated with post-ablation atrial fibrillation(AF)recurrence.Methods 207 patients were enrolled and completed in this prospective observational study.Patients with AF scheduled for receive radiofrequency catheter ablation(RFCA)therapy were screened for the study.Before ablation therapy,electrocardiogram,24 h holter monitor,transesophageal echocardiography,serum cystatin C,high-sensitivity C-reactive protein,creatinine levels,and routine blood examinations were examined.After ablation,patients were followed up every week for the first month,and then at 2,3,6,9,and 12 months.Thereafter,patients came back to out-patient clinic every six months regularly.Electrocardiogram or 24 h holter monitor were repeated if the patient experienced palpitations or every six months.AF recurrence was defined as atrial flbrillation/atrial flutter or atrial tachycardia lasting≥30 seconds within three months after therapy.Results Compared to patients with no AF recurrence,patients with recurrence had longer AF history(P=0.007),more early recurrence(P=0.000),a larger left atrium(P=0.004),and higher pre-ablation cystatin C levels(P=0.000).Multivariate regression analysis revealed that cystatin C and left atria(LA)diameter were risk factors for AF recurrence.After adjusting for LA diameter,the risk of AF recurrence increased 30%with every milligram cystatin C elevation(95%CI:1.117-1.523).Conclusions Pre-ablation cystatin C levels were associated with AF recurrence after RFCA therapy,an optimal cut-off value of 1.190 mg/L(sensitivity=0.576;specificity=0.851).

关键词： Atrial fibrillation Catheter ablation cystatin C Recurrence

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Serum cystatin C levels are negatively correlated with post-stroke cognitive dysfunction

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Neural Regeneration Research 2020年第5期15卷 922-928页

作者： Dao-Xia Guo Zheng-Bao Zhu Chong-Ke Zhong Xiao-Qing Bu Li-Hua Chen Tan Xu Li-Bing Guo Jin-Tao Zhang Dong Li Jian-Hui Zhang Zhong Ju Chung-Shiuan Chen Jing Chen Yong-Hong Zhang Jiang He Department of Epidemiology School of Public Health and Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric DiseasesMedical College of Soochow UniversitySuzhouJiangsu ProvinceChina Department of Epidemiology Tulane University School of Public Health and Tropical MedicineNew OrleansLAUSA Department of Neurology Siping Central HospitalSipingJilin ProvinceChina Department of Neurology the 88th Hospital of People’s Liberation ArmyTaianShandong ProvinceChina Department of Internal Medicine Feicheng City People’s HospitalFeichengShandong ProvinceChina Department of Neurology Tongliao Municipal HospitalInner Mongolia Autonomous RegionChina Department of Neurology Kerqin District First People’s Hospital of Tongliao CityInner Mongolia Autonomous RegionChina Department of Medicine Tulane University School of MedicineNew OrleansLAUSA

Stroke is the leading cause of death and long-term disability worldwide,and cognitive impairment and dementia are major complications of ischemic stroke.cystatin C (CysC) has been found to be a neuroprotective factor in animal studies.However,the relationship between CysC levels and cognitive dysfunction in previous studies has revealed different results.This prospective observational study investigated the correlation between serum CysC levels and post-stroke cognitive dysfunction at 3 months.Data from 638 patients were obtained from the China Antihypertensive Trial in Acute Ischemic Stroke (CATIS).Cognitive dysfunction was assessed using the Mini-Mental State Examination (MMSE) at 3 months after stroke.According to the MMSE score,308 patients (52.9%) had post-stroke cognitive dysfunction.After adjusting for potential confounding factors,the odds ratio (95% CI) of post-stroke cognitive dysfunction for the highest quartile of serum CysC levels was 0.54 (0.30–0.98),compared with the lowest quartile.The correlation between serum CysC and cognitive dysfunction was modified by renal function status.We observed a negative linear dose-response correlation between CysC and cognitive dysfunction in patients with normal renal function (Plinearity = 0.044),but not in those with abnormal renal function.Elevated serum CysC levels were correlated with a low risk of 3-month cognitive dysfunction in patients with acute ischemic stroke,especially in those with normal renal function.The current results suggest that CysC is a protective factor for post-stroke cognitive dysfunction,and could be used to treat post-stroke cognitive dysfunction.The CATIS study was approved by the Institutional Review Boards at Soochow University from China (approval No.2012-02) on December 30,2012,and was registered at ClinicalTrials.gov (identifier No.NCT01840072) on April 25,2013.

关键词： abnormal renal function cognitive dysfunction cystatin C ischemic stroke Mini-Mental State Examination neural regeneration neuroprotective effect normal renal function

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Role of serum cystatin C in the prediction of contrast-induced nephropathy after intra-arterial interventions

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Chinese Medical Journal 2020年第4期133卷 408-414页

作者： Zheng-Yu Wang Yong-Li Wang Jian Wei Long Jin Zhen-Chang Wang Department of Radiology Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China Department of Interventional Radiology The Sixth People's Hospital South CampusShanghai Jiao Tong UniversityShanghai 201499China

Background:The diagnosis of contrast-induced nephropathy(CIN)is usually based on changes in serum creatinine(sCr).However,sCr has poor sensitivity as a biomarker of kidney injury.The aim of this study was to investigate the usefulness of serum cystatin C(sCysC)to predict CIN after intra-arterial interventions.Methods:A total of 360 consecutive patients underwent intra-arterial procedures using digital subtraction angiography.SCr,sCysC,and estimated glomerular filtration rate were measured at 1 to 2 days before and at 48,72 h,and 7 days after the procedure.Results:Thirty-one patients(8.61%)developed CIN.Receiver operating characteristic(ROC)curve analysis showed that preoperative sCysC levels had good discriminatory power(area under the curve[AUC]=0.634;95%confidence interval[CI]=0.526-0.743)for evaluating the risk of CIN after an endovascular procedure,with a sensitivity of 53.33%and specificity of 73.70%.ROC analysis showed that sCysC at 48 h after contrast medium administration was predictive of CIN after an endovascular procedure(AUC=0.735;95%CI=0.647-0.822)with satisfactory sensitivity of 74.20%and specificity of 63.90%.Diabetes mellitus was an independent risk factor for CIN(odds ratio=2.778;95%CI=1.045-7.382;P=0.040).Conclusions:SCysC is an appropriate biomarker to predict the occurrence of CIN.Baseline sCysC before an intervention is useful to obtain a preliminary estimate of the risk of CIN.A 48-h cut-off value of sCysC of 0.99 mg/L after an endovascular procedure may help to rule out patients at lower risk of CIN.

关键词： Contrast-induced nephropathy Intra-arterial intervention Serum cystatin C Serum creatinine

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cystatin C is a biomarker for predicting acute kidney injury in patients with acute-on-chronic liver failure

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World Journal of Gastroenterology 2013年第48期19卷 9432-9438页

作者： Zhi-Hong Wan Jian-Jun Wang Shao-Li You Hong-Ling Liu Bing Zhu Hong Zang Chen Li Jing Chen Shao-Jie Xin Liver Failure Treatment and Research Center Beijing 302 Hospital

AIM:To investigate serum cystatin C level as an early biomarker for predicting acute kidney injury(AKI)in patients with acute-on-chronic liver failure(ACLF).METHODS:Fifty-six consecutive patients with hepatitis B virus-related ACLF who had normal serum creatinine(Cr)level(cystatin C(CysC)was performed by a particle-enhanced immunonephelometry assay using the BN Prospec nephelometer system.The ACLF patients were followed during their hospitalization period.RESULTS:In the ACLF group,serum level of CysC was 1.1±0.4 mg/L,which was significantly higher(P<0.01)than those in the healthy controls(0.6±0.3mg/L)and CHB patients(0.7±0.2 mg/L).During the hospitalization period,eight ACLF patients developed AKI.Logistic regression analysis indicated that CysC level was an independent risk factor for AKI development(odds ratio=1.8;95%CI:1.4-2.3,P=0.021).The cutoff value of serum CysC for prediction of AKI in ACLF patients was 1.21 mg/L.The baseline CysC-based estimated glomerular filtration rate(eGFR CysC)was significantly lower than the creatinine-based eGFR(eGFR CG and eGFR MDRD)in ACLF patients with AKI,suggesting that baseline eGFR CysC represented early renal function in ACLF patients while the Cr levels were still within the normal ranges.CONCLUSION:Serum CysC provides early prediction of renal dysfunction in ACLF patients with a normal serum Cr level.

关键词： Acute-on-chronic liver failure cystatin C Creatinine Acute kidney injury Prediction

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定量检测血和尿中cystatin C含量在肾移植患者肾功能评价中的应用

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细胞与分子免疫学杂志 2010年第11期26卷 1140-1142页

作者：马樱许晓光黄海燕李琦宋朝君石炳毅金伯泉第四军医大学免疫学教研室陕西西安710032 解放军第309医院器官移植中心北京100091

目的:建立定量检测半胱氨酸蛋白酶抑制剂C(cysC)水平的夹心ELISA方法,对肾移植患者的肾功进行监测,以期作为术后急性排斥反应(AR)及肾功能延迟恢复(DGF)发生的一项新的预警指标。方法:建立分泌抗人cys C单克隆抗体(mAb)的杂交瘤细胞株,... 详细信息

目的:建立定量检测半胱氨酸蛋白酶抑制剂C(cysC)水平的夹心ELISA方法,对肾移植患者的肾功进行监测,以期作为术后急性排斥反应(AR)及肾功能延迟恢复(DGF)发生的一项新的预警指标。方法:建立分泌抗人cys C单克隆抗体(mAb)的杂交瘤细胞株,制备腹水纯化mAbs并进行辣根过氧化物酶标记,建立检测cys C的夹心ELISA方法;应用此方法检测正常人及23例肾移植患者移植手术前后的血清(Scys C)及尿(Ucys C)标本中cys C的含量并分析其与血清肌酐(SCR)的相关性。结果:获得7株可稳定分泌抗cys CmAb的杂交瘤细胞。应用建立的ELISA方法检测cys C含量敏感度可达0.1μg/L。肾移植正常恢复(NC)组患者Scys C及Ucys C含量与SCR下降趋势一致。术后发生AR的患者Ucys C水平持续较高,直到术后21 d才出现下降。而发生DGF的患者同NC组患者相比术后4周内Scys C及Ucys C与SCR维持在较高水平,Ucys C的水平有逐渐升高的趋势,直到术后60 d才下降到与NC相近的水平。结论:建立了敏感稳定的检测cys C含量的ELISA方法,可用于检测Ucys C水平。而Scys C及Ucys C的持续升高可能提示肾移植患者术后AR或DGF的发生。

关键词： cystatin C 肾移植夹心ELISA 肾功能

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重组cystatin对冷藏鲢鱼的抑菌效果

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食品与生物技术学报 2019年第5期38卷 111-118页

作者：钟海霞李冉李树红杨娟胡强柯勤勤林灵白稚子李美良四川农业大学食品学院

分离鉴定4℃有氧冷藏鲢鱼去皮肉片货架期终点腐败相关优势假单胞菌,并研究鲢鱼重组cystatin(家族Ⅱ)对优势腐败假单胞菌的抑菌效果。首先利用CFC培养基从4℃有氧冷藏鲢鱼去皮肉片货架期终点,分离筛选出腐败相关假单胞菌,并采用生理生化... 详细信息

分离鉴定4℃有氧冷藏鲢鱼去皮肉片货架期终点腐败相关优势假单胞菌,并研究鲢鱼重组cystatin(家族Ⅱ)对优势腐败假单胞菌的抑菌效果。首先利用CFC培养基从4℃有氧冷藏鲢鱼去皮肉片货架期终点,分离筛选出腐败相关假单胞菌,并采用生理生化和16 SrDNA法对其进行鉴定,其次将腐败菌株进行回接试验,通过测定各腐败菌回接组的挥发性盐基氮(TVB-N)值、菌落数、感官评分判断其致腐能力。最后,采用滤纸片扩散法和营养肉汤法鉴定重组鲢鱼cystatin(家族Ⅱ)对优势腐败假单胞菌的抑菌效果。结果表明,荧光假单胞菌(pseudomonas fluorescens)和草莓假单胞菌(Pseudomonas fragi)为主要致腐菌,所占比例分别为63.3%、26.7%,且荧光假单胞菌致腐能力强于草莓假单胞菌。重组cystatin对两种菌均有明显的抑菌效果,且呈剂量依赖关系。

关键词：鲢鱼 4℃有氧冷藏优势假单胞菌分离鉴定 cystatin 抑菌作用

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cystatin M在乳腺癌中的表达及其临床意义

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江苏医药 2007年第3期33卷 233-235页

作者：魏江朱江罗光华汤艳红牟琴峰陈陆俊董选常州市第一人民医院综合实验室 213003

目的了解cystatin M在乳腺肿瘤中的表达及其临床意义。方法采用实时定量PCR检测52例乳腺肿瘤标本及邻近正常乳腺组织中cystatin M mRNA及内参GAPDH表达水平,分析cystatin M基因表达与临床病理参数的关系。结果乳腺癌标本的cystatin M含... 详细信息

目的了解cystatin M在乳腺肿瘤中的表达及其临床意义。方法采用实时定量PCR检测52例乳腺肿瘤标本及邻近正常乳腺组织中cystatin M mRNA及内参GAPDH表达水平,分析cystatin M基因表达与临床病理参数的关系。结果乳腺癌标本的cystatin M含量与其周围邻近的正常乳腺组织及良性肿瘤中表达cystatin M含量无统计学意义;但发生淋巴结转移的乳腺肿瘤标本的cystatin M则明显低于其周围邻近的正常乳腺组织(Pcystatin M表达水平与乳腺癌病人的雌激素受体状态(ER)及孕激素状态(PR)无关。结论cystatin M是一种监测乳腺癌是否发生转移的一种可靠的指标。

关键词： cystatin M 乳腺癌转移

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cystatin C Peptide Effects on B16F10 Melanoma Cells

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CellBio 2021年第1期10卷 1-9页

作者： Mathieu Hill James L. Cox AT Still University Department of Biochemistry West Jefferson Kirksville Missouri USA

Background: Metastasis is a major problem for effective therapy of cancer. Small cysteine protease inhibitors, cystatins have been shown to be anti-metastatic for a number of different cancers. We have identified a small peptide of cystatin which exhibits anti-cancer properties for B16 melanoma cells in vitro. Methods: B16 melanoma cells were measured for growth, proliferation, migration, and apoptosis in the presence and absence of cystatin peptide. Results: The cystatin peptide reduced melanoma cell growth, proliferation, migration, and increased apoptosis in melanoma cells in culture. Conclusion: cystatin peptide exhibits anti-cancer effects on highly metastatic B16 melanoma cells in culture.

关键词： cystatin Peptide Melanoma Metastasis

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小桐子cystatin家族基因和相应miRNAs的鉴定及其在低温响应中可能的作用

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植物生理学报 2021年第2期57卷 347-361页

作者：吴丹丹陈永坤杨宇孔春艳龚明云南师范大学生命科学学院生物能源持续开发利用教育部工程研究中心云南省生物质能与环境生物技术重点实验室昆明650500

半胱氨酸蛋白酶抑制剂(cystatin)是一组抑制植物中木瓜类半胱氨酸蛋白酶C1A和豆类蛋白酶C13活性的小分子蛋白。小桐子(Jatropha curcas)是一种具有开发潜力的能源植物及冷敏植物,其cystatin基因家族及作用目前尚不清楚。本研究在全基因... 详细信息

半胱氨酸蛋白酶抑制剂(cystatin)是一组抑制植物中木瓜类半胱氨酸蛋白酶C1A和豆类蛋白酶C13活性的小分子蛋白。小桐子(Jatropha curcas)是一种具有开发潜力的能源植物及冷敏植物,其cystatin基因家族及作用目前尚不清楚。本研究在全基因组水平上对小桐子cystatin基因家族及靶向该家族成员的miRNAs进行了鉴定,并对cystatin家族系统进化、保守结构域、染色体定位、表达特性及成员基因与miRNAs互作参与调控小桐子对低温的响应进行了解析。结果表明,小桐子基因组中共鉴定到5个cystatin基因(命名为JcCYS1~5),定位到6、8和10号染色体,可分为3个亚家族(A、B、C),编码106~242个氨基酸,理论等电点4.97~9.17,序列比对发现cystatin的结构域基本保守且位于N端。表达分析显示,JcCYS1在种子、花蕾、叶、茎端表达量较高,而JcCYS2在授粉期间表达量高。基于miRNA组和降解组测序结果,发现有10个miRNAs可能靶向调控cystatin基因家族的3个成员JcCYS1、JcCYS2和JcCYS5。RT-qPCR分析表明,小桐子cystatin家族的5个基因的表达水平在12°C冷锻炼期间总体呈现出显著响应,尤其是在冷锻炼早期(12~24 h)。此外,对靶向JcCYS2和JcCYS5的miRNAs在冷锻炼过程中的表达分析表明,miR11036-y、miR396-z与JcCYS2以及miR8154-x与JcCYS5的表达在冷锻炼期间呈明显负相关,暗示着这些miRNAs的确参与了cystatin基因的调控,且这种调控应该与冷锻炼诱导的小桐子耐冷性有关。这些结果有助于更好了解小桐子cystatin家族功能及其与相应miRNAs的互作、以及这种互作如何调控小桐子对低温胁迫的响应。

关键词：小桐子 cystatin 基因家族 miRNAs 低温响应

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cystatin F在中枢神经系统脱髓鞘动物模型中的表达

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神经解剖学杂志 2009年第2期25卷 159-162页

作者：李菲菲范凯张艳丽宋大为马坚妹大连医科大学解剖学教研室大连116044

神经脱髓鞘疾病是中枢神经系统难治性疾病,髓鞘脱失后伴有各种基因表达的异常。明确这些基因的表达形式对脱髓鞘疾病的治疗具有重要意义。本研究利用0.2%cuprizone饲育C57BL/6小鼠6周制备中枢神经脱髓鞘模型,采用原位杂交技术对组织蛋... 详细信息

神经脱髓鞘疾病是中枢神经系统难治性疾病,髓鞘脱失后伴有各种基因表达的异常。明确这些基因的表达形式对脱髓鞘疾病的治疗具有重要意义。本研究利用0.2%cuprizone饲育C57BL/6小鼠6周制备中枢神经脱髓鞘模型,采用原位杂交技术对组织蛋白酶抑制剂cystatin F的mRNA表达进行研究。结果发现,正常野生型小鼠中枢神经系统中未见cystatin F mRNA的表达,而大量表达于脱髓鞘模型小鼠白质内。通过小胶质细胞标志物Iba1抗体免疫组化和c-fms的原位杂交研究发现,脱髓鞘模型小鼠白质内有大量激活的小胶质细胞。为进一步确定表达cystatin F的细胞类型,通过cystatin F原位杂交后Iba1免疫双染技术,确定cystatin F在中枢神经主要由激活的小胶质细胞表达,提示其表达与小胶质细胞活化和崩解髓鞘残骸清除有关。

关键词：脱髓鞘 cystatin F 小胶质细胞

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