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Effect of prairie milk vetch (Astragalus adsurgens Pall) cultured root on proliferation of Stellera chamaejasme Linn

Effect of prairie milk vetch (Astragalus adsurgens Pall) cul...

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中国第二届植物化感作用学术研讨会暨中国植物保护学会植物化感作用专业委员会成立大会

作者： YU Fuke MA Yongqing Institute of Soil and Water Conservation Chinese Academy of SciencesMinistry of Water Resource Graduate School of Chinese Academy of Sciences Northwest Sci-Tech University of Agriculture & Forestry

The effect of prairie milk vetch (Astragalus adsurgens Pall) root culture filtrates extracts with ethyl acetate was studied by using cultured shoot of Stellera chamaejasme Linn as receptor. The result showed that virtual propagation, elongation and dry weight accumulation of S. chamaejasme cultured shoot was stimulated by adding 6.4 mg/L the extract to the growth media, but the fresh weight accumulation of S. chamaejasme was inhibited. When the extract was added at the level of 3.2, 12.7, 25.4 mg/L, the virtual propagation, elongation and dry weight accumulation of S. chamaejasme cultured shoot was inhibited obviously. The more the extraction was used, the more intensive the inhibitory effect became. The effect of the extracts used at these 3 levels on fresh weight accumulation of S. chamaejasme cultured shoot is also inhibitive. The inhibitive effect was increased then decreased, with the increasing of the extracts. Moreover, this study indicated that the inhibitive or stimulative effects of prairie milk vetch root culture filtrates extracts with ethyl acetate on S. chamaejasme cultured shoot were due to chemical secretion from the cultured root. It has proved that prairie millk vetch has allelopathic effect on S. chamaejasme.

关键词： prairie milk vetch (Astragalus adsurgens Pall) cultured root culture filtrate Stellera chamaejasme L. proliferation allelopathic effect

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ALLELOPATHIC RESPONSE OF CUCURBITA MOSCHATA *** rootS TO NUTRIENT ELEMENTS REGULATION

ALLELOPATHIC RESPONSE OF CUCURBITA MOSCHATA *** ROOTS TO NUT...

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中国第三届植物化感作用学术研讨会、第八届全国杂草科学大会、联合国粮农组织——中国“水稻化感作用论坛”

作者： Cui-ping YUAN ~1,Yong-qing MA~(1,2**),Jun-feng SHUI~2 1 College of Resources and Environment,Northwest A&F University,Yangling 712100,Shaanxi province,China 2 Institute of Soil and Water Conservation,Chinese Academy of Sciences, Yangling 712100,Shaanxi,China

Based on the combination of plant culture technique and allelopathic bioassay,allelopathic response and dynamic modes of Cucurbila moschata *** roots was conducted through regulating kinds and contents of selective mass element and micro-elements in B culture base, under four concentration series（1/2 B5,B5,3/2 B5,and 2 B5） for five necessary elements(P,Mg,

关键词： Cucurbita moschata M.var.Jingou cultured root allelopathic effects nutrient regulation response mechanism

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Optimal culture of prairie milk vetch root and measurement on its allelopathic effect

Optimal culture of prairie milk vetch root and measurement o...

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中国第二届植物化感作用学术研讨会暨中国植物保护学会植物化感作用专业委员会成立大会

The objective of this research was to determine the optimum concentration of sucrose, macro-elements, micro-elements and organic supplements for the propagation of prairie milk vetch (Astragalus adsurgens Pall) roots on B5 media. An orthogonal design was used to test multiple factors. A second objective was to test for allelopathic effect of the prairie milk vetch roots by conducting a bioassay using the extracts isolated from culture filtrates with ethyl acetate after culturing the roots. The results indicated that strength macro-elements and strength micro-elements with exception of standard concentration of MnSO4 and strength basic organic supplements in the B5 medium with addition of 3.0% sucrose and 1.074 umol l-1 NAA is the optimal medium for the propagation of prairie milk vetch root. A bioassay using the culture filtrate extracts indicated that the roots might have produced allelopathic chemicals during their culture. Differences in the degree of allelopathic effects suggest that the nutrient stress may influence the production of the allelopathic chemicals. This study provides a basis for the improvement of root regeneration and propagation in prairie milk vetch and suggests that nutrition may influence the production of allelopathic chemicals by this plant.

关键词： allelopathic effect culture filtrate cultured root extract optimal culture prairie milk vetch.

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