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Studies on Electrical Activation of Porcine Oocytes Matured in vitro and Embryo culture Systems

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Agricultural Sciences in China 2002年第10期1卷 1168-1173页

作者： WU Zhong-hong, XING Feng-ying, LIU Guo-shi, ZENG Shen-ming, ZHU Shi-en, ZHANG Zhong-cheng and FU Peng-hui(College of Animal Science and Technology, China Agricultural University, Beijing 100094 , P.R. China) College of Animal Science and Technology China Agricultural University Beijing 100094 P.R.China

Conditions for electrical parthenogenetic activation of porcine oocytes matured in vitro and in vitro culture systems of porcine embryo were studied. The best results were achieved under the conditions of electrical field strength and the pulse duration at 130Vmm-1/80 us, with a blastocyst development rate of (20.12 ± 8.18) % (P > 0.05). No significant difference was found between treatments of multiple pulses and a single pulse (P > 0.05). Parthenogenetic embryos were cultured with different methods and air conditions for 7 days in vitro, blastocyst development rate of embryos with changed culture media [ (26.44 ± 8.35) % ] or changed media with 10% fetal bovine serum (FBS) [ (17.68 ± 5.39)% ] on the fifth day showing no significant difference from that of embryos without change of culture media [ (25.30 ± 7.55) % , P > 0.05 ], while cell numbers of blastocysts from embryos with changed culture media (15.78 + 5.46 and 14.55 ± 4.81) were significantly lower than number of blastocysts from embryos without change of culture media (18.01 ± 6.79, Pcultured in lower O2(5%CO2: 7%O2:88%N2) also showed no significant difference from those in high O2(5%CO2 in air) [(20.78 ± 8. 80)% and 17.0016.12 vs. (25.30 ± 7.55)% and 18.0116.79, P>0.05]. It is concluded that change of culture media with the same new one or changing over to media with 10% fetal bovine serum (FBS) on the fifth day and low O2 environment are not necessary for porcine embryos development.

关键词： Porcine Oocytes culture in vitro Electrical activation Embryo culture

In vitro culture of Bonamia ostreae and Its Identification

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Animal Husbandry and Feed Science 2014年第4期6卷 206-208页

作者： Feng Chunyan Wang Fei Yang Lianru Wu Shaoqiang Institute of Animal Quarantine Chinese Academy of Inspection and Quarantine College of Animal Science and Veterinary Medicine Inner Mongolia Agricultural University

In recent years,bonamiosis has frequently occurred in European areas,which has caused the death of oyster in a wide range and brought enormous economic losses to the breeding industry of oyster. Nowadays,the study on Bonamia sp. is still in the elementary phase. The technology of pathogen’s culture in vitro is the basis for further study on the pathogenesis of Bonamia sp.,its interaction with hosts and the prevention and control of the related disease. In this study,total tissues of oyster identified by PCR were used as culture media to in vitro culture. After one month,they were identified by the method of in situ hybridization. It was found that the results of in situ hybridization were accordant with PCR results. And Bonamia ostreae were detected by in situ hybridization after B. ostreae were cultured for one month. We successfully established a simple and feasible method for in vitro culturing B. ostreae.

关键词： Bonamia ostreae culture in vitro Identification In situ hybridization

Cultivation, identification and quantification of one species of yeast-like symbiotes, Candida, in the rice brown planthopper, Nilaparvata lugens

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Insect Science 2012年第4期19卷 477-484页

作者： Kun Pang Sheng-Zhang Dong Yun Hou Ya-Lin Bian Ke Yang Xiao-Ping Yu Zhejiang Provincial Key Laboratory of Biometrology and Inspection&Quarantine Technique College of Life SciencesChina Jiliang UniversityHangzhouChina

The brown planthopper （BPH）, Nilaparvata lugens Stal, which is one of the most destructive pests of rice, has been confirmed to harbor yeast-like symbiotes （YLS） in the fat body. Several morphologically different YLS have been previously isolated and cultured in vitro from BPH, but direct evidence is lacking to further clarify whether the cultured YLS were from BPH. In this study, one species of YLS was successfully cultured in vitro and simultaneously verified to exist in the fat body of BPH by sequence analysis and nested polymerase chain reaction （PCR）. The cultured YLS isolate in vitro was identified as a member of the genus Candida on the basis of 18S rDNA （ribosomal DNA） and 5.8S-ITS （internal transcribed spacer） rDNA sequence and a phylogenetic analysis of ITS sequences from yeast. Therefore, this yeast isolate was named as Candida-like symbiotes. Candida-like symbiotes was found to exist in fat bodies, ovaries and newly laid eggs of the BPH, but not in the heads, thoraxes and mid-guts. In addition, the number of Candida- like symbiotes in 1×106 of purified YLS from BPH fat bodies was speculated to be （5.32±0.22）×104 on the basis of a quantitative PCR analysis.

关键词： Candida-like symbiotes culture in vitro identification Nilaparvata lugens,yeast-like symbiotes

Comparison of biological characteristics of marrow mesenchymal stem cells in hepatitis B patients and normal adults

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World Journal of Gastroenterology 2007年第11期13卷 1743-1746页

作者： Liang Peng Hua Li Lin Gu Xiao-Mou Peng Yang-Su Huang Zhi-Liang Gao Department of Infectious Diseases The Third Affiliated Hospital of Sun Yat-Sen University Guangzhou 510630 Guangdong Province China

AIM: To establish a culture system of marrow mesenchymal stem cells (MSCs)from hepatitis B patients and normal adults and to compare their biological characteristics. METHODS: MSCs were isolated from bone marrow in 34 male hepatitis B patients and 15 male normal adults and cultivated in vitro. Their biological characteristics including surface markers, shapes and appearances, growth curves, first passage time and passage generations were compared. RESULTS: Cultivation achievement ratio of hepatitis B patients was lower than that of normal adults, no statistical significance (82.35% vs 100%, P > 0.05). Compared with MSCs of normal adults, MSCs of hepatitis B patients presented a statistical lower growth curve, longer first passage time (13.0 ± 1.6 d vs 11.4 ± 1.5 d, P cultured in vitro. Although their appearance, shape and surface marker are similar to those of MSCs from normal adults, there are differences in their biological characteristics.

关键词： Marrow mesenchymal stem cells culture in vitro Biological characters

Establishment of primary cultures of craniopharyngioma cells

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Neural Regeneration Research 2012年第8期7卷 601-605页

作者： Hao Liu Liang Liu Zhiyong Liu Qiang Li Chao You Jianguo Xu Department of Neurosurgery West China Hospital Sichuan University Chengdu 610041 Sichuan Province China Department of Neurosurgery Affiliated Hospital of Luzhou Medical College Luzhou 646000 Sichuan Province China

Craniopharynigoma samples were collected from 36 patients. Out of the 36 samples, 29 achieved successful sub-culturing, with a success rate of 80.6%. Immunohistochemistry staining showed that cytokeratin-7 was positively expressed in the cytomembrane and cytoplasm of craniopharyngioma cells at 6-8 passages, confirming that all cultured cells were squamous epithelial cells. The doubling time of craniopharyngioma cells was 3 days, as confirmed by the 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay. In this study, craniopharyngioma cells cultured in vitro were established; however, establishment of immortalized craniopharyngioma cell lines requires further research.

关键词： craniopharyngioma cytokeratin-7 primary culture culture in vitro cell line

日本血吸虫尾蚴经人工方法转变的童虫体外培养的研究

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Current Zoology 1987年第2期33卷 144-150页

作者：王薇周述龙湖北医学院寄生虫学教研室湖北医学院血吸虫痛研究室武汉

本文介绍了日本血吸虫体外培养系统。建立了较适于童虫生长发育的B41培养基。尾蚴经人工方法转变的童虫在体外可发育至雌雄合抱,雌雄生殖器官形成。雌虫可达产卵阶段,但未具备正常的产卵机能。培养的血吸虫在体外至少可存活110天。

关键词： Schistosoma japonicum Cercaria Schistosomulum culture in vitro culture medium.

Study on Virus-free and Rapid Propagation Technology of Artemisia selengensis *** Yangxin County

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Agricultural Biotechnology 2020年第6期9卷 70-72,78页

作者： Shunwen XU Yihe LI Anhuai MING Rongxing YAN Xianai GAO Jin ZHENG Wei KANG Hubei Key Laboratory of Mine Environmental Pollution Control and Remediation Hubei Polytechnic UniversityHuangshi 435003China School of Environmental Science and Engineering Hubei Polytechnic UniversityHuangshi 435003China Vegetable Office of Yangxin Huangshi 435200China Vegetable Research Institute of Huangshi Huangshi 435003China

[Objectives]This study was conducted to culture virus-free tissue culture plantlets of mid-maturing green-stalk Artemisia selengensis *** in Yangxin County.[Methods]With bud tips of *** in Yangxin as explants and MS as the basal medium,screening and proportioning of plant growth regulators were performed to establish a virus-free and rapid propagation system for mid-maturing green-stalk varieties of *** in Yangxin.[Results]The optimal callus induction medium,adventitious shoot differentiation medium,adventitious shoot elongation medium and rooting medium for explants were MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+sucrose 25 g/L+agar 7 g/L,MS+6-BA 0.1 mg/L+NAA 0.1 mg/L+sucrose 25 g/L+agar 7 g/L,MS+6-BA 0.02 mg/L+NAA 0.02 mg/L+sucrose 25 g/L+agar 7 g/L and 1/2 MS+NAA 0.05 mg/L+sucrose 25 g/L+agar 7 g/L,respectively,and the seedling rate reached more than 95%.The culture conditions were as follows:temperature(25±2)℃,relative humidity 85%,illumination intensity 3000 lx,and illumination time 14 h/d.[Conclusions]This study has important practical significance for the purification and rejuvenation and large-scale industrial breeding of Yangxin *** seedlings.

关键词： Artemisia selengensis sp. culture in vitro Virus free Rapid propagation

Effects of Three Drugs on Membrane Metabolism Against Cysticerci cellulosae in vitro

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Journal of Northeast Agricultural University(English Edition) 2009年第3期16卷 32-36页

作者： HAO Yanhong LI Qingzhang GAO Xuejun LIU Yongjie GAO Wenxue College of Veterinary Medicine Northeast Agricultural University Harbin 150030 China

To investigate the effects and mechanisms of the benzimidazole carbamate and benmidine drugs on Cysticerci cellulosae and choose effective drugs on Cysticerci cellulosae, the membrane metabolism of Cysticerci cellulosae in vitro was tested after three kinds of drugs which were used respectively. The indexes included the contents of lipids, the contents of SA and the changes of the membrane fluidity. The results showed that oxfendazole could inhibit the membrane metabolism of immature and mature Cysticerci cellulosae in vitro, and albendazole only inhibited the mature one, while thibendimidine neither acted on the immature nor mature one.

关键词： Cysticerci cellulosae culture in vitro benzimidazole carbamate benmidine membrane metabolism

Effects of Electrical Stimulation on Growth and Metabolism of Cardiomyocytes in vitro

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Effects of Electrical Stimulation on Growth and Metabolism o...