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Biodegradation of crystal violet by Agrobacterium radiobacter

Journal of Environmental Sciences 2011年第8期23卷 1384-1393页

作者： G.K.Parshetti S.G.Parshetti A.A.Telke D.C.Kalyani R.A.Doong S.P.Govindwar Department of Biomedical Engineering and Environmental Sciences National Tsinghua University Division of Applied Life Sciences Gyeongsang National University Division of Chemical Engineering Konkuk University Department of Biochemistry Shivaji University

Agrobacterium radiobacter MTCC 8161 completely decolorized the crystal violet with 8 hr （10 mg/L） at static anoxic conditions. The decreased decolorization capability by A. radiobacter was observed, when the crystal violet concentration was increased from 10 to 100 mg/L. Semi-synthetic medium containing 1% yeast extract and 0.1% NH4C1 has shown 100% decolorization of crystal violet within 5 hr. A complete degradation of crystal violet by A. radiobacter was observed up to 7 cycles of repeated addition （10 rag/L）. When the effect of increasing inoculum concentration on decolorization of crystal violet （100 mg/L） was studied, maximum decolorization was observed with 15% inoculum concentration. A significant increase in the activities of laccase （184%） and aminopyrine N-demethylase （300%） in cells obtained after decolorization indicated the involvement of these enzymes in decolorization process. The intermediates formed during the degradation of crystal violet were analyzed by gas chromatography and mass spectroscopy （GC/MS）. It was detected the presence of N,N,N＇,N＂-tetramethylpararosarfiline, IN, N-dimethylaminophenyl] [N-methylaminophenyl] benzophenone, N, N-dimethylaminobenzaldehyde, 4-methyl amino phenol and phenol. We proposed the hypothetical metabolic pathway of crystal violet biodegradation by A. radiobacter. Phytotoxicity and microbial toxicity study showed that crystal violet biodegradation metabolites were less toxic to bacteria （A. radiobacter, P. aurugenosa and A. vinelandii） contributing to soil fertility and for four kinds of plants （Sorghum bicolor, Vigna radiata, Lens culinaris and Triticum aestivum） which are most sensitive, fast growing and commonly used in Indian agriculture.

关键词： Agrobacterium radiobacter crystal violet biodegradation gas chromatography and mass spectroscopy toxicity study

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Energy transfer in solid-state dye lasers based on methyl methacrylate co-doped with sulforhodamine B and crystal violet

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Chinese Physics B 2013年第11期22卷 319-324页

作者： R.G.Geethu Mani M.Basheer Ahamed Department of Physics KCG College of Technology Research Centre Bharathiar University Department of Physics B.S. Abdur Rahman University

Laser action in methyl methacrylate （MMA） co-doped with sulforhodamine B and crystal violet dyes was investi- gated. The dye mixture was incorporated into a solid polymeric matrix and was pumped by a 532-nm Nd：YAG laser. Distributed feedback dye laser （DFDL） action was induced in the dye mixture using a prism arrangement both in the donor and acceptor regions by an energy transfer mechanism. Theoretically, the characteristics of acceptor and donor DFDLs, and the dependence of their pulse widths and output powers on acceptor-donor concentrations and pump power, were studied. Experimentally, the output energy of DFDL was measured at the emission peaks of donor and acceptor dyes for different pump powers and different acceptor-donor concentrations. Tuning of the output wavelength was achieved by varying the period of the gain modulation of the laser medium. The laser wavelength showed continuous tunability from 563 nm to 648 nm.

关键词： sulforhodamine B crystal violet distributed feedback dye laser solid state distributed feedbackdye laser

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Degradation of crystal violet using nanometer TiO_2 under the synergistic action of H_2O_2 and ultrasonic wave

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Rare Metals 2004年第4期23卷 352-357页

作者： GUOXiaojun YUANLi QIYouli LIXien YANGWu GAOJinzhang ChemistryandChemicalEngineeringCollege NorthwestNormalUniversityLanzhou730070China

TiO_2 nanoparticles with different phases are prepared by hydrolysis oftitanium tetrabutoxide in the presence of HC1. The composition and microstructure of the resultingsamples are studied by XRD and TEM. These results show that the range of particle size of TiO_2 isfrom 20 to 30 nm. The mechanism of TiO_2 photocatalysis reaction has been discussed *** activities of nanometer TiO_2 are also evaluated by degradation of the crystal violetsolution. Experimental results indicate that the synergistic action of H_2O_2 and ultrasonic wavegreatly enhances photo-catalytic reaction of TiO_2.

关键词： inorganic nonmetallic materials photocatalysis synergistic effect TiO_2nanoparticles crystal violet

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Removal of crystal violet from aqueous solution using powdered mycelial biomass of Ceriporia lacerata P2

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Journal of Environmental Sciences 2011年第12期23卷 2055-2062页

作者： Yonghui Lin Xingbing He Guomin Han Qijian Tian Wenyong Hu College of Biology and Environmental Sciences Jishou University Jishou 416000 China School of Life Science Nanjing University Nanjing 210093 China

A biosorbent prepared from powdered mycelial biomass of Ceriporia lacerata （CLB）, a basidiomycetous fungus, was applied for the uptake of crystal violet from aqueous solution. A batch adsorption experiment was used for the biosorption process, involving effect of experimental factors and biosorption kinetics and equilibrium. Biosorption process showed that the removal of crystal violet by CLB was effective over wide pH range, and meanwhile was independent on ionic strength. Biosorption capacities of CLB increased with the initial dye concentration increasing, due to an increase in the driving force of the concentration gradient. The adsorbed crystal violet amount per unit biomass weight decreased with increasing biosorbent dosage, due to the splitting effect of flux （concentration gradient） between sorbate and biosorbent. A maximum sorption capacity of 239.25 mg/g was observed. Biosorption kinetics was found to be best represented by the pseudo second-order kinetic model. The equilibrium adsorption data was well described by the Koble-Corrigan model. FT-IR （Fourier Transform Infrared Spetroscopy） spectrum showed the presence of O–H, COOH, C=O, C–N, C–H, –NH2 and P–OH in the surface of CLB as functional groups. This study showed CLB can effectively remove CV from dye wastewater.

关键词： biosorption Ceriporia lacerata P2 crystal violet equilibrium kinetics

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Biosorption and biotransformation of crystal violet by Aeromonas hydrophila DN322p

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Frontiers of Environmental Science & Engineering 2013年第2期7卷 185-190页

作者： Tao PAN Suizhou REN Jun GUO Meiying XU Guoping SUN School of Bioscience and Bioengineering South China University of Technology Guangzhou 510006 China Guangdong Institute of Microbiology Guangzhou 510070 China Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application Guangdong Institute of Microbiology Guangzhou 510070 China Guangdong Open Laboratory of Applied Microbiology Guangzhou 510070 China State Key Laboratory of Applied Microbiology (Ministry-Guangdong Province Jointly Breeding Base) South China Guangzhou 510070 China

DN322p, an offspring of Aeromonas hydro- phila DN322, has the capacity to adsorb and decolorize triphenylmethane dyes in wastewater simultaneously. As a common triphenylmethane dye, crystal violet （CV） was chosen to test the decolorization characteristics of DN322p. Within 0.5h, the strain DN322p adsorbed a large amount of CV, producing a deep-colored cell pellet and colorless supernatant. The colors of the cell pellet and supematant lightened over time. The supernatant and dichloromethane extract of the cell pellet both showed conspicuous CV and leuco CV （LCV） characteristic absorbance peaks at 590 nm and 260 nm, respectively, in the UV-vis spectral analysis. This finding indicated that the DN322p cells can adsorb the two dyes. A 99% （w/w） decolorization rate was achieved within 2.5 h with shaking at 30℃ for 50mg CV.L-1. High Performance Liquid Chromatography （HPLC） analysis of the dichloromethane extract of the supernatant and cell pellet confirmed that CV was mainly converted into its leuco form. Dead cells had a similar adsorption capacity with living cells. About 90% of CV in the dye solution （50mg-L-1） was removed by autoclaved cells with an optical delnsity at 600 nm （OD600） above 1.0.

关键词： crystal violet decolorization biosorption,biotransformation Aeromonas hydrophila DN322p

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The ultratrace detection of crystal violet using surface enhanced Raman scattering on colloidal Ag nanoparticles prepared by electrolysis

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Chinese Chemical Letters 2009年第6期20卷 711-715页

作者： Ren Ming Liu Yi Pu Kang Xing Fa Zi Ming Jun Feng Man Cheng Min Zhen Si Application Institute of Spectroscopy Technology Chuxiong Normal University Chuxiong 675000 China

Highly active, stable and affordable surface enhanced Raman scattering （SERS） substrates were obtained by electrolyzing a mixture of AgNO3 （4 × 10^-4 mol/L） and Na3C6H5OTH2O （6 × 10^-5 mol/L） for 1, 2, 3 and 4 h at 7 V. With crystal violet （CV） as a test molecule, a portable Raman spectrometer with 785 nm laser excitation was employed to carry out the SERS detection. Colloidal Ag nanoparticles prepared by electrolyzing for 3 h with the particle size of （65 ±17） nm is a perfect SERS substrate for the ultratrace detection of CV, which displayed an enhancement factor of ca. 1.3 × 10^8 and the detection limit of CV is down to ca. 10-15 mol/L （ca. 10^-4 ppb） with 10^-1 mol/L KBr as aggregating agent. Thus, this SERS substrate will provide a hopeful foreground in ultratrace detection. Meanwhile, it will provide a possibility to bring Raman analysis out of the laboratory to process in situ, real-time detection and identification.

关键词： SERS Ultratrace detection crystal violet Silver nanoparticle Electrolysis

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Molecularly Imprinted Polymers for Selective Extraction of crystal violet from Natural Seawater coupled with HighPerformance Liquid Chromatographic Determination

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Journal of Ocean University of China 2014年第2期13卷 236-242页

作者： LIAN Ziru WANG Jiangtao Key Laboratory of Marine Chemistry Theory and Technology Ministry of EducationOcean University of China Marine College Shandong University at Weihai

Molecularly imprinted polymers （MIPs） were prepared by the bulk polymerization using crystal violet as the template molecule, and the methacrylic acid and ethylene glycol dimetheacrylate as functional monomer and cross-linker, respectively. Sys- tematic investigations of synthetic conditions were conducted. The surface morphology and recognition mechanism of the obtained polymers were studied using scanning electron microscope and spectrophotometric analysis. MIPs showed high atTmity to template molecule and were successfully applied as special solid-phase extraction sorbent for selective extraction of crystal violet from natural seawater. An off-line molecularly imprinted solid-phase extraction （MISPE） method followed by high-performance liquid chroma- tography with diodearray detection for the analysis of crystal violet was also established. MISPE columns have good recoveries for crystal violet standard solutions and good linearity was obtained over the concentration range of 0-200 ~tg L-1 （R2 〉 0.99）. Finally, two natural seawater samples were investigated. The recoveries of spiked seawater on the MISPE columns were from 44.47% to 62.34%, the relative standard deviation （n=3） being in the range of 2.89%-5.96%.

关键词： crystal violet molecularly imprinted polymer adsorption solid-phase extraction natural seawater

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Extraction-spectrophotometric Determination of Dicyanoaurate(Ⅰ) with crystal violet

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Rare Metals 1998年第4期17卷 59-62页

作者：罗明标张燮黄文艳

Extraction spectrophotometric determination of dicyanoaurate (Ⅰ) with crystal violet is described. At pH 2.6～10.7, dicyanoaurate (Ⅰ) can react with crystal violet and form a complex which can be extracted into isoamylacetate. The apparent molar absorptivity is 8.37×10 4 L·mol -1 ·cm -1 at 605 nm. Beer′s law is obeyed for 0～15 μg of Au(Ⅰ) per 5 ml of isoamylacetate. The interference from Ag(Ⅰ) and Cu(Ⅱ) can be eliminated with thiourea and EDTA. The method is applied to the determination of dicyanoaurate (Ⅰ) in sample solution with satisfactory results.

关键词： Extraction photometry crystal violet Gold (Ⅰ)

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Influence Factors and Kinetics on crystal violet Degradation by Fenton and Optimization Parameters using Response Surface Methodology

Influence Factors and Kinetics on Crystal Violet Degradation...

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International Conference on Environmental and Agriculture Engineering（ICEAE 2011）

作者： Chengyuan Sua b Yong Wanga a. School of Municiple and Environmental Engineering Harbin Institute of Technology Harbin China b. School of Environment and Resources Guangxi Normal University The Guangxi Key Lab of Environmental Engineering Protection and Assessment Guilin China

This work investigated influence factors and kinetics on crystal degradation violet dye by Fenton. For FeSO4 dose, fitted the date of crystal violet concentration and time, it conformed to first-order kinetic. At 0.1ml H2O2, decolorization rate was 98.2% and it increased to 98.7% when H2O2 was raised to 0.2ml at 15 min. However, H2O2 doses higher than 0.4ml, no obvious increased in the removal was observed. An important pH effect was found, reaching its degradation at pH 2.0 with decolorization rate of 97% at 30min. However, when pH was 4 and 6, the removal was only 79% and 72% at 40min. The Box-Behnken Response Surface Methodology was utilized for mathematical modeling for decolorization rate as a response by Fenton. The determinate coefficient of model was 0.9851. The optimum operating conditions were that FeSO4 dose was 4.55ml, H2O2 dose was 0.20ml and reaction time was 15min. Under such conditions, the error of the model predicted and experimental values was 1.85%.

关键词： Response surface methodology Box-Behnken design crystal violet decolorization

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Study on UV/Fenton/Heteropoly Acid Oxidation Processes Used in the Degradation of crystal violet

Study on UV/Fenton/Heteropoly Acid Oxidation Processes Used ...

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The 12th Asian Pacific Confederation of Chemical Engineering Congress

作者： Li Songtian Gao Mei Ma Wei Xue Jingjiao School of Chemistry and Chemical Engineering Pingdingshan Uinversity,467000 Pingdingshan,Ghina

Photo-Fenton reaction due to a large number of hydroxyl radical to oxidative degradation of organic matter,heteropolyacid has strong UV absorption properties,can be used as the activation of Fenton *** study for the end of crystal violet,inspected the UV/Fenton/heteropoly- acid system on the degradation of dye through the initial pH value of the option,HO cousump-

关键词： fenton reagents UV catalytic oxidation heteropolyacid crystal violet

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