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A unique photosystem I reaction center from a chlorophyll d-containing cyanobacterium Acaryochloris marina

Journal of Integrative Plant Biology 2021年第10期63卷 1740-1752页

作者： Caihuang Xu Qingjun Zhu Jing-Hua Chen Liangliang Shen Xiaohan Yi Zihui Huang Wenda Wang Min Chen Tingyun Kuang Jian-Ren Shen Xing Zhang Guangye Han Department of Biophysics and Department of Pathology of Sir Run Run Shaw Hospital Zhejiang University School of MedicineHangzhou 310058China Photosynthesis Research Center Key Laboratory of PhotobiologyInstitute of BotanyThe Chinese Academy of SciencesBeijing 100093China College of Life Sciences University of Chinese Academy of ScienceBeijing 100049China Center of Cryo-Electron microscopy Zhejiang University School of MedicineHangzhou 310058China School of Life and Environmental Science Faculty of ScienceUniversity of SydneySydney NSW2006Australia Division of Photosynthesis and Structural Biology Institute for Interdisciplinary Scienceand Graduate School of Natural Science and TechnologyOkayama UniversityOkayama 700-8530Japan

Photosystem I(PSI)is a large protein supercomplex that catalyzes the light-dependent oxidation of plastocyanin(or cytochrome c6)and the reduction of ferredoxin.This catalytic reaction is realized by a transmembrane electron transfer chain consisting of primary electron donor(a special chlorophyll(Chl)pair)and electron acceptors A_(0),A_(1),and three Fe_(4)S_(4) clusters,F_(X),F_(A),and F_(B).Here we report the PSI structure from a Chl d-dominated cyanobacterium Acaryochloris marina at 3.3Åresolution obtained by single-particle cryo-electron microscopy.The A.marina PSI exists as a trimer with three identical monomers.Surprisingly,the structure reveals a unique composition of electron transfer chain in which the primary electron acceptor A_(0) is composed of two pheophytin a rather than Chl a found in any other well-known PSI structures.A novel subunit Psa27 is observed in the A.marina PSI structure.In addition,77 Chls,13α-carotenes,two phylloquinones,three Fe-S clusters,two phosphatidyl glycerols,and one monogalactosyl-diglyceride were identified in each PSI monomer.Our results provide a structural basis for deciphering the mechanism of photosynthesis in a PSI complex with Chl d as the dominating pigments and absorbing far-red light.

关键词： A.marina chlorophyll d cryoelectron microscopy pheophytin a photosynthesis photosystem I reaction center

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Introduction to 3D reconstruction of macromolecules using single particle electron microscopy

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Acta Pharmacologica Sinica 2005年第10期26卷 1153-1164页

作者： Oscar LLORCA Centro de lnvestigaciones Biológicas Consejo Superior de Investigaciones Científicas Ramiro de Maeztu 9 Campus Universidad Complutense 28040 Madrid Spain

Single-particle electron microscopy has now reached maturity, becoming a commonly used method in the examination of macromolecular structure. Using a small amount of purified protein, isolated molecules are observed under the electron microscope and the data collected can be averaged into a 3D reconstruction. Single-particle electron microscopy is an appropriate tool for the analysis of proteins that can only be obtained in modest quantities, like many of the large complexes currently of interest in biomedicine. Whilst the use of electron microscopy expands, new methods are being developed and improved to deal with further challenges, such as reaching higher resolutions and the combination of informa tion at different levels of structural detail. More importantly, present methodology is still not robust enough when studying certain "tricky" proteins like those displaying extensive conformational flexibility and a great deal of user expertise is required, posing a threat to the consistency of the final structure. This mini review describes a brief outline of the methods currently used in the 3D analysis of macromolecules using single-particle electron microscopy, intended for those first approaching this field. A summary of methods, techniques, software, and some recent work is presented. The spectacular improvements to the technique in recent years, its advantages and limitations compared to other structural methods, and its future developments are discussed.

关键词： single particle electron microscopy 3D reconstruction cryoelectron microscopy image processing DNA-PK

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