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Comparison of three transgenic Bt rice lines for insecticidal protein expression and resistance against a target pest, Chilo suppressalis （Lepidoptera： Crambidae）

引用

Insect Science 2016年第1期23卷 78-87页

作者： Ya-Nan Wang Kai-Qie Ke Yun-He Li Lan-Zhi Han Yan-Min Liu Hong-Xia Hua Yu-Fa Peng State Key Laboratory for Biology of Plant Disease and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural ScienceBeUing 100193 College of Plant Science & Technology Huazhong Agricultural University Wuhan 430070 China

Two transgenic rice lines （T2A-1 and T1C-19b） expressing cry2A and crylC genes, respectively, were developed in China, targeting lepidopteran pests including Chilo suppressalis （Walker）（Lepidoptera： Crambidae）. The seasonal expression of Cry proteins in different tissues of the rice lines and their resistance to C. suppressalis were assessed in comparison to a Bt rice line expressing a crylab/ac fusion gene, Huahui 1, which has been granted a biosafety certificate. In general, levels of Cry proteins were T2A- 1 〉 Huahui I 〉 T1C-19b among rice lines, and leaf 〉 stern 〉 root among rice tissues. The expression patterns of Cry protein in the rice line plants were similar： higher level at early stages than at later stages with an exception that high Cry 1C level in T 1 C- 19b stems at the maturing stage. The bioassay results revealed that the three transgenic rice lines exhibited significantly high resistance against C. suppressalis larvae throughout the rice growing season. according to Cry protein levels in rice tissues, the raw and corrected mortalities of C. suppressalis caused by each Bt rice line were the highest in the seedling and declined through the j ointing stage with an exception for T1 C-19b providing an excellent performance at the maturing stage. By comparison, T1C-19b exhibited more stable and greater resistance to C. suppressalis larvae than T2A-1, being close to Huahui 1. The results suggest crylC is an ideal Bt gene for plant transformation for lepidopteran pest control, and T1C-19b is a promising Bt rice line for commercial use for tolerating lepidopteran rice pests.

关键词： Chilo suppressalis crylab/ac cryl C ery2A pest resistance transgenic rice

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