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Absolute copy number differences of Y chromosomal genes between crossbred (Bos taurus×Bos indicus) and Indicine bulls

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Journal of Animal Science and Biotechnology 2013年第4期4卷 257-263页

作者： Ayan Mukherjee Gulshan Dass Jagan Mohanarao G Moloya Gohain Biswajit Brahma Tirtha Kumar Datta Sachinandan De Animal Genomics Lab Animal Biotechnology Centre National Dairy Research Institute Department of Veterinary Biochemistry College of Veterinary Sciences & Animal Husbandry Central Agricultural University

Background： The Y chromosome in mammal is paternally inherited and harbors genes related to male fertility and spermatogenesis. The unique intra-chromosomal recombination pattern of Y chromosome and morphological difference of this chromosome between Bos taurus and Bos indicus make it an ideal model for studying structural variation, especially in crossbred （Bos taurus x Bos indicus） bulls. Copy Number Variation （CNV） is a type of genomic structural variation that gives information complementary to SNP data. The purpose of this study was to find out copy number differences of four Y chromosomal spermatogenesis-related candidate genes in genomic DNA of crossbred and purebred Indicine bulls. Result： Four Y chromosomal candidate genes of spermatogenesis namely, sex determining gene on Ychromosome （SRY）, DEAD box po/ypeptide 3-Y chromosome （DDX3 Y）, Ubiquidn specific peptidase 9, Y-linked （ usPgY）, testis-specific protein on Y chromosome （TSPY） were evaluated. Absolute copy numbers of Y chromosomal genes were determined by standard curve-based quantitative real time PCR. Copy numbers of SRYand TSPYgenes per unit amount of genomic DNA are higher in crossbred than Indicine bulls. However, no difference was observed in DDX3Yand usPgYgene copy numbers between two groups. Conclusion： The present study demonstrates that the structural organization of Y chromosomes differs between crossbred and Indicine bulls which are reproductively healthy as observed from analysis of semen attributes. The absolute copy numbers of SRY and TSPY genes in unit mass of genomic DNA of crossbred bulls are significantly higher than Indicine bulls. No alteration in absolute copies ofDDX3Yand usPgYgene was found between the genome of crossbred and Indicine bulls. This study suggests that the DDX3Yand USPgYare likely to be single copy genes in the genome of crossbred and Indicine bulls and variation in Y chromosome length between crossbred and Indicine bulls may be due to the copy number variation of

关键词： Absolute copy SRY DDX3Y usPgY TSPY crossbred

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Factors affectingin-vivo fertility of crossbred Egyptian - Italian buffalo semen

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Asian pacific Journal of Reproduction 2017年第6期6卷 264-267页

作者： GA El-Sisy KGhM Mahmoud AAE El-sokary MF Nawito YF Ahmed Department of Animal Reproduction and A.I. National Research CenterDokkiGizaEgypt General Organization for Veterinary Services DokkiEgypt

Objective:To assess the effect of region, season and year of insemination onin-vivo fertility of Italian-Egyptian crossbred buffalo ***: A total number of 4799 female buffaloes were inseminated by frozen semen with at least 50% post-thaw motility of Egyptian-Italian crossbred bulls in three localities in Delta, lower Egypt (El-Behira, El-Sharkia and Damietta) during the period of 2013, 2014 and 2015. The pregnancy rate after two months was evaluated during the four ***:The rate of pregnancy was significantly (Pcrossbred Egyptian-Italian buffalo semen.

关键词： Artificial insemination Pregnancy rate Egyptian-Italian crossbred Buffalo bull

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