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resistance development,cross-resistance,and fitness costs associated with Aphis gossypii resistance towards sulfoxaflor and acetamiprid in different geographical regions

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Journal of Integrative Agriculture 2024年第7期23卷 2332-2345页

作者： Wei Wang Renfu Zhang Haiyang Liu Ruifeng Ding Qiushi Huang Ju Yao Gemei Liang Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis of Ministry of Agriculture and Rural Affairs Institute of Plant ProtectionXinjiang Academy of Agricultural SciencesUrumqi 830091China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China

Aphis gossypii has become increasingly difficult to manage due to its strong insecticide resistance.In the laboratory,we established sulfoxaflor-resistant and acetamiprid-resistant strains in two A.gossypii populations with different basal insecticide resistance levels,and evaluated the effects of basal insecticide resistance on the resistance development and cross-resistance,as well as differences in fitness.Under the same selection pressure,Yarkant A.gossypii(with low basal insecticide resistance)evolved resistance to sulfoxaflor and acetamiprid more quickly than Jinghe A.gossypii(with high basal insecticide resistance),and the evolution of A.gossypii resistance to sulfoxaflor developed faster than acetamiprid in both Yarkant and Jinghe,Xingjiang,China.The sulfoxaflor-resistant strains selected from Yarkant and Jinghe developed significant cross-resistance to acetamiprid,imidacloprid,thiamethoxam and pymetrozine;while the acetamiprid-resistant strains developed significant cross-resistance to sulfoxaflor,imidacloprid,thiamethoxam,pymetrozine,and chlorpyrifos.The relative fitness of A.gossypii decreased as the resistance to sulfoxaflor and acetamiprid developed.The relative fitness levels of the sulfoxaflor-resistant strains(Yarkant-SulR and Jinghe-SulR)were lower than those of the acetamipridresistant strains(Yarkant-AceR and Jinghe-AceR).In addition,the relative fitness levels of sulfoxaflor-and acetamiprid-resistant strains were lower in Jinghe than in Yarkant.In summary,basal insecticide resistance of A.gossypii and insecticide type affected the evolution of resistance to insecticides in A.gossypii,as well as cross-resistance to other insecticides.The sulfoxaflor-and acetamiprid-resistant A.gossypii strains had obvious fitness costs.The results of this work will contribute to the insecticide resistance management and integrated management of A.gossypii.

关键词： Aphis gossypii sulfoxaflor acetamiprid cross-resistance fitness costs different sensitivities

Does cotton bollworm show cross-resistance to the Bacillus thuringiensis toxins Cry1Ac and Cry2Ab? A mini review

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Journal of Arid Land 2020年第2期12卷 349-356页

作者： MA Jihong TIAN Changyan LYU Guanghui MAI Wenxuan State Key Laboratory of Desert and Oasis Ecology Xinjiang Institute of Ecology and GeographyChinese Academy of SciencesUrumqi 830011China University of Chinese Academy of Sciences Beijing 100049China College of Resources and Environment Science Xinjiang UniversityUrumqi 830046China

Since 1996, transgenic Bacillus thuringiensis(Bt) cotton has been commercially grown in numerous countries in an effort to stem the losses caused by key lepidopteran pests. However, the development of pest resistance to Bt toxins has jeopardized the continued utilization of Bt cotton. As a strategy designed to circumvent the development of resistance, Bt cotton varieties expressing two or more toxins targeting the same pest have been introduced. Nevertheless, from the perspective of long-term planting of Bt cotton, the potential risk of cross-resistance to these Bt toxins is a threat that cannot be ignored. In this paper, we review current research(including that based on the analysis of protein binding sites and resistance genes) on the resistance of cotton bollworm(Helicoverpa armigera) to the Bt toxins Cry1 Ac and Cry2 Ab and the interrelationship between these toxins. On the basis of existing evidence, we assume that the actions of Cry1 Ac and Cry2 Ab against cotton bollworm are not completely independent, and then propose the "resistance-associated gene mutation potential hypothesis". Although the mechanisms underlying the resistance of pests to Bt toxins are yet to be comprehensively elucidated, this hypothesis could undoubtedly have important implications for adopting "pyramid" strategy in the future. Further research is recommended to devise strategies to retard the development of H. armigera resistance to Bt cotton, either using different Bt toxins or their various combinations.

关键词： cross-resistance genetically modified cotton cotton bollworm Bacillus thuringiensis Cry1Ac Cry2Ab

cross-resistance pattern to four AHAS-inhibiting herbicides of tribenuron-methyl-resistant flixweed(Descurainia sophia) conferred by Asp-376-Glu mutation in AHAS

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Journal of Integrative Agriculture 2016年第11期15卷 2563-2570页

作者： DENG Wei YANG Qian JIAO Hong-tao ZHANG Yong-zhi LI Xue-feng ZHENG Ming-qi Department of applied Chemistry China Agricultural University Beijing 1000193 P.R.China

Flixweed（Descurainia sophia L.） is a problematic and widespread weed in winter wheat fields and has been controlled by tribenuron-methyl for more than twenty years in China. In this study, a flixweed accession（Hebei 25, HB25） with an Asp-376-Glu mutation in acetohydroxy acid synthase（AHAS） was identified and purified. The purified HB25 accession（p HB25） developed 758.1-fold resistance to tribenuron-methyl and exhibited obvious cross-resistance to four AHAS-inhibiting herbicides. The resistant/susceptible（R/S） ratios of 50% plant growth reduction（GR_（50）） to herbicides of halosulfuron-methyl, flumetsulam, imazethapyr and pyribenzoxim were 346.1, 15.7, 8.1 and 7.1, respectively. The reduced AHAS sensitivities to four different AHAS-inhibiting herbicides, which were caused by the Asp-376-Glu mutation, were responsible for the resistance and cross-resistance to AHAS-inhibiting herbicides. The R/S ratios of 50% inhibition of AHAS activity（I50） to tribenuron-methyl, halosulfuron-methyl, flumetsulam, imazethapyr and pyribenzoxim were 844.5, 532.9, 74.5, 13.3 and 5.5, respectively. The results of AHAS activity in vitro were highly correlated with that of whole-plant response experiments.

关键词： flixweed AHAS cross-resistance Asp-376-Glu mutation

resistance and fitness costs in diamondback moths after selection using broflanilide, a novel meta-diamide insecticide

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Insect Science 2022年第1期29卷 188-198页

作者： Xi Sun Rui Wei Linhong Li Bin Zhu Pei Liang Xiwu Gao Department of Entomology China Agricultural UniversityBeijing100193China

The diamondback moth (DBM) Plutella xylostella (L.) (Lepidoptera: Plutellidae) is an insect pest found around the world that feeds on cruciferous crops. The DBM has become resistant to most insecticides in current use in the field. Broflanilide is a novel meta-diamide insecticide that binds to a new site on the γ-aminobutyric acid receptor and very efficiently protects against most pests in the order Lepidoptera, including DBM. In this study, the resistance of a laboratory-bred susceptible strain of DBM to broflanilide and the fitness costs posed by broflanilide to the DBM were evaluated. The DBM had no obvious resistance to broflanilide after 10 generations of selection. The realized heritability h2 was 0.033, suggesting a low risk of resistance developing in this strain. The F10 generation had no cross-resistance to the insecticides abamectin and endosulfan (which target the γ-aminobutyric acid receptor) and chlorantraniliprole (which targets a non-γ-aminobutyric acid receptor). The specific activities of important detoxification enzymes (cytochrome P450 monooxygenase, esterase, and glutathione S-transferase) were not obviously altered. However, the larval stage was prolonged and the adult stage was shortened significantly in F11 generation than the F0 generation. The total preoviposition period TPOP significantly prolonged 1.90 d in F11 generation. The fitness value Rf (0.93) was lower for the F11 generation than the F0 generation. The results indicated that long-term exposure to broflanilide exerts clear fitness costs in the DBM. This information will be useful in identifying reasonable broflanilide application guidelines for managing broflanilide resistance in the DBM.

关键词： broflanilide cross-resistance detoxifying enzymes fitness cost Plutella xylostella

Comparative study of the effect of solvents on the efficacy of neonicotinoid insecticides against malaria vector populations across Africa

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Infectious Diseases of Poverty 2022年第2期11卷 23-31页

作者： Magellan Tchouakui Tatiane Assatse Leon M.J.Mugenzi Benjamin D.Menze Daniel Nguifo-Nguete Williams Tchapga Jonathan Kayondo Francis Watsenga Emile Zola Manzambi Michael Osae Charles S.Wondji Centre for Research in Infectious Diseases(CRID) P.O.Box 13501YaoundeCameroon Parasitology and Ecology Laboratory Department of Animal Biology and PhysiologyFaculty of ScienceUniversity of Yaounde 1P.O.Box 812YaoundeCameroon Institut National de Recherche Biomedicale KinshasaDemocratic Republic of Congo Radiation Entomology and Pest Management Centre Biotechnology and Nuclear Agriculture Research InstituteGhana Atomic Energy CommissionP.O.Box LG80Legon-AccraGhana Department of Vector Biology Liverpool School of Tropical MedicinePembroke PlaceLiverpool L35QAUK International Institute of Tropical Agriculture(IITA) P.O.Box 2008YaoundeCameroon Entomology Department Uganda Virus Research Institute(UVRI)P.O.Box 49EntebbeUganda.

Background:New insecticides with a novel mode of action such as neonicotinoids have recently been recommended for public health by WHO.resistance monitoring of such novel insecticides requires a robust protocol to monitor the development of resistance in natural populations.In this study,we comparatively used three different solvents to assess the susceptibility of malaria vectors to neonicotinoids across Africa.Methods:Mosquitoes were collected from May to July 2021 from three agricultural settings in Cameroon(Njombe-Penja,Nkolondom,and Mangoum),the Democratic Republic of Congo(Ndjili-Brasserie),Ghana(Obuasi),and Uganda(Mayuge).Using the CDC bottle test,we compared the effect of three different solvents(ethanol,acetone,MERO)on the efficacy of neonicotinoids againstAnopheles gambiae s.l.In addition,TaqMan assays were used to genotype key pyrethroid-resistant markers inAn.gambiae and odds ratio based on Fisher exact test were used to evaluate potential cross-resistance between pyrethroids and clothianidin.Results:Lower mortality was observed when using absolute ethanol or acetone alone as solvent for clothianidin(11.4-51.9% mortality in Nkolondom,31.7-48.2% in Mangoum,34.6-56.1% in Mayuge,39.4-45.6% in Obuasi,83.7-89.3% in Congo and 71.1-95.9% in Njombe pendja)compared to acetone+MERO for which 100% mortality were observed for all the populations.Similar observations were done for imidacloprid and acetamiprid.Synergist assays(PBO,DEM and DEF)with clothianidin revealed a significant increase of mortality suggesting that metabolic resistance mechanisms are contributing to the reduced susceptibility.A negative association was observed between the L1014F-kdr mutation and clothianidin resistance with a greater frequency of homozygote resistant mosquitoes among the dead than among survivors(OR=0.5;P=0.02).However,the I114T-GSTe2 was in contrast significantly associated with a greater ability to survive clothianidin with a higher frequency of homozygote resistant among survivors than other genotypes(OR=2.10;P=0.013).Conc

关键词： Malaria Anopheles Insecticide resistance Neonicotinoids Clothianidin cross-resistance

<i>gyrA</i>Gene Mutation Conferring Phenotypic cross-resistance among Fluoroquinolones (Ofloxacin, Levofloxacin and Gatifloxacin) in Multidrug Resistant <i>Mycobacterium tuberculosis</i>Strains Isolated from Pulmonary MDR-TB Patients in Bangladesh

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Journal of Tuberculosis Research 2018年第3期6卷 227-237页

作者： Tamanna Tasnim Fatema Mohammad Alam S. M. Ali Ahmed Shirin Tarafder S. M. Mostofa Kamal Shamim Hossain Department of Microbiology and Immunology Bangabandhu Sheikh Mujib Medical University (BSMMU) Dhaka Bangladesh National Tuberculosis Reference Laboratory (NTRL) National Institute of Diseases of the Chest and Hospital (NIDCH) Dhaka Bangladesh

Background and objectives: Fluoroquinolones (FLQs) are an essential component of multidrug resistant-tuberculosis (MDR-TB) treatment regimen but unfortunately the emergence of FLQ resistant MDR-TB cases is challenging the current MDR-TB treatment regimen. FLQ resistance is mainly caused by gyrA gene mutation and phenotypic cross-resistance may occur among the different FLQs. A limited number of data exists regarding the cross-resistance phenomenon among FLQs and it appears that resistance to the present class representative FLQ, ofloxacin (OFX), may or may not correlate with complete cross-resistance to other FLQs. So the study was designed to observe if gyrA gene mutations confer to the phenotypic cross-resistance among FLQs [OFX, Levofloxacin (LFX) and Gatifloxacin (GFX)] tested. Methodology: Sputum samples from 68 diagnosed pulmonary MDR-TB cases were collected. All samples were subjected to Multiplex Real-time PCR for the detection of gyrA gene mutations and conventional culture on Lowenstein-Jensen (L-J) media followed by drug sensitivity testing (DST) of culture isolates (MDR-TB strains) by indirect proportion method for the detection of phenotypic resistance pattern to OFX, LFX and GFX. Results: Out of the 68 MDR-TB sputum samples 13 (19.11%) had MDR-TB bacilli with mutations in the gyrA gene and 11(16.18%) of the MDR-TB culture isolates were found to have resistance to FLQs by DST. The study observed that 11 MDR-TB samples with gyrA gene mutations showed complete phenotypic cross-resistance among OFX, LFX and GFX. Conclusion: This study found that mutation in the gyrA gene of the MDR-TB bacilli results in the complete cross-resistance among the FLQs (OFX, LFX and GFX) tested. It is therefore of utmost importance to carry out the base line resistance and cross-resistance tests for the individual FLQs prior to initiating the treatment of MDR-TB cases in Bangladesh for successful clinical outcomes.

关键词： Multidrug Resistant-Tuberculosis (MDR-TB) gyrA Gene Mutations Fluoroquinolones (FLQs) cross-resistance

Detection of resistance and cross-resistance to Four ALS-inhibiting Herbicides of Scirpus juncoides Roxb. in Paddy Field in Heilongjiang Province

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Journal of Northeast Agricultural University(English Edition) 2019年第2期26卷 1-14页

作者： Liu Ya-guang Zhang Zheng-nan Shi Hui Zhu Jin-wen Wu Hui-peng College of Agriculture Northeast Agricultural UniversityHarbin 150030China College of Agricultural Science and Biotechnology Zhejiang UniversityKey Laboratory of Molecular Biology of Crop and PestMinistry of AgricultureHangzhou 310029China

The resistance and cross-resistance of Scirpus juncoides Roxb.to ALS-inhibiting herbicides were tested to provide theoretical basis for effectively control Scirpus juncoides Roxb.and rational use of herbicides.To identify the resistance and crossresistance of 19 populations from nine areas in Heilongjiang Province to four ALS-inhibiting herbicides by agar method and the whole-plant assay.The results are as the followings,the two bioassay methods had good correlation,the resistance indexes in the same area were different by two means,but resistant level was the same,similarity up to 89.5%.The resistant rates to pyrazosulfuronethyl,bensulfuron-methyl,penoxsulam and pyribenzoxim were 26.3%,36.8%,36.1%and 21.1%,respectively.Shangzhi populations evolved the highest level of resistance to pyrazosulfuron-methyl with a resistance index of 15.56.Shangzhi,Tonghe,Fangzheng and Jixi populations showed cross-resistance to four ALS-inhibiting herbicides,accounting for 26.3%.The resistance of Scirpus juncoides Roxb.to four ALS-inhibiting herbicides in Heilongjiang paddy field was low to moderate,five populations showed cross-resistance to four ALS-inhibiting herbicides.

关键词： Scirpus juncoides Roxb. ALS-inhibiting herbicide resistance cross-resistance

Deltamethrin:Promising mosquito control agent against adult stage of Aedes aegypti L.

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Asian Pacific Journal of Tropical Medicine 2011年第6期4卷 430-435页

作者： Sarita Kumar Anita Thomas Pillai MKK Department of Zoology Acharya Narendra Dev College(University of Delhi)New Delhi-110019India

Objective:To evaluate the effects of deltamethrin against field-collected adults of Aedes aegypti L(Ae.aegypti).Methods:The adults were selected with 0.025%deltamethrin for 40 successive generations.The selected adults were tested with 4%DDT and the emerging larvae were tested with various insecticides to study the cross-resistance spectrum.The knockdown and irritability studies were carried out in adult mosquitoes to investigate their behavioural response to deltamethrin.Results:Forty generations of selection with deltamethrin resulted in only 3.8-fold resistance in the adults of Ae.aegypti.The adults of parent(PS) and selected strains (DAS) exhibited only 0.8-fold cross resistance to 4%DDT.The larvae emerging from the PS and DAS strains did not develop appreciable levels of resistance to various insecticides tested.The knockdown studies revealed KTSO of 14.4 min in PS adults with no signs of recovery even after 24 h. The DAS strains could develop only 1.2 to 1.3-fold knockdown resistance(KDR).The knockdown response of DDT was though 5-6 times slower than deltamethrin but the continued response in deltamethrin-selected adults caused only 1.2-fold KDR.The PS and DAS strains exhibited significant irritability response towards deltamethrin and DDT.The DAS strains showed 5-6 fold increased irritability to deltamethrin as compared to the PS strain.Conclusions:The above results suggest the prolonged effective use of deltamethrin against Ae.aegypti as an adulticide.

关键词： Deltamethrin Knockdown Irritability resistance cross-resistance

Non-target-site and target-site resistance to AHAS inhibitors in American sloughgrass(Beckmannia syzigachne)

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Journal of Integrative Agriculture 2018年第12期17卷 2714-2723页

作者： WANG Jing-jing LI Xiang-ju LI Dan HAN Yu-jiao LI Zheng YU Hui-lin CUI Hai-lan Institute of Plant Protection Chinese Academy of Agricultural Sciences

American sloughgrass(Beckmannia syzigachne(Steud.) Fernald) is one of the most competitive and malignant weeds in rice-wheat rotation fields in China. American sloughgrass populations in the Jiangsu Province of China became less sensitive to acetohydroxyacid synthase(AHAS) inhibitors after repeated application for many years in these areas. Two suspected resistant American sloughgrass populations(R1 and R2) collected in the field were detected the resistance to inhibitors of AHAS in whole-plant dose-response assays, compared to the susceptible(S) population. These assays indicated that R1 showed low resistance to mesosulfuron-methyl(3.32-fold), imazapic(2.84-fold) and pyroxsulam(1.55-fold), moderate resistance to flazasulfuron(4.67-fold) and pyribenzoxim(7.41-fold), and high resistance to flucarbazone(11.73-fold). However, using a combination of the cytochrome P450 inhibitor, malathion, with mesosulfuron-methyl resulted in a reduction in R1 resistance relative to mesosulfuron-methyl alone. Furthermore, R2 was highly resistant to flazasulfuron(34.90-fold), imazapic(11.30-fold), flucarbazone(49.20-fold), pyribenzoxim(12.94-fold), moderately resistant to mesosulfuron-methyl(9.77-fold) and pyroxsulam(6.26-fold), and malathion had no effect on R2 resistance to mesosulfuron-methyl. The fulllength of AHAS genes was sequenced and the AHAS enzymes were assayed in vitro in order to clarify the mechanism of resistance to AHAS inhibitors in R1 and R2 populations. The results demonstrated that R2 had a Pro-197-Ser mutation in the AHAS gene, and the sensitivity of R2 to the five AHAS inhibitors was decreased, which may result in R2 resistance to AHAS inhibitors. There was no mutation in the AHAS gene of R1, and there were no significant differences in enzyme sensitivity between susceptible(S) and resistant(R1) populations. An enhanced metabolism may be the main mechanism of R1 resistance to AHAS inhibitors.

关键词： American sloughgrass cross-resistance gene mutation non-target-site resistance