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Anti-viral activity of Staphylococcus aureus lysates against herpes simplex virus type-Ⅰinfection:an in vitro and in vivo study

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International Journal of Ophthalmology(English edition) 2021年第10期14卷 1463-1472页

作者： Tian-Lan Lin Chao Cheng Wei-Ting Zeng Fang Duan Yin-Hui Pei Xiu-Ping Liu Fu Shang Kai-Li Wu Zhongshan Ophthalmic Center State Key Laboratory of OphthalmologySun Yat-sen UniversityGuangzhou 510060Guangdong ProvinceChina

AIM:To investigate the effect of Staphylococcus aureus(***)lysates(SALs)on herpes simplex virus type-Ⅰ(HSV1)infection in human corneal epithelial(HCE)cells and in a mouse model of HSV1 ***:HCE,Vero,HeLa,and BV2 cells were infected with HSV1[HSV1f strain,HSV1f;HSV-1-H129 with green fluorescent protein(GFP)knock-in,HSV1g].Pre-or post-infection,SAL at various concentrations was added to the culture medium for 24 *** fluorescence in HSV1g or plaque formation by HSV1f were *** effects of heat-treated SAL,precooled acetone-precipitated SAL,and SAL subjected to ultrafiltration(100 kDa)were *** effects of other bacterial components and lysates on HSV1 infection were also tested,including lipoteichoic acid(LTA),peptidoglycan(PGN),staphylococcal protein A(SPA),andα-hemolysin from ***(α-toxin)as well as lysates from a wild-type *** strain,***,and Escherichia coli(W-SAL,SEL,and ECL,respectively).In addition,SAL eye drops were applied topically to BALB/c mice with HSV1 keratitis,followed by in vivo ***:The cytopathic effect,plaque formation(HSV1f),and GFP expression(HSV1g)in infected cells were inhibited by SAL in a dose-dependent *** active component of SAL(≥100 kDa)was heat-sensitive and retained activity after acetone *** HSV1ginfected cells,treatment with LTA-sa,α-toxin,PGN-sa,or SPA did not inhibit GFP ***,W-SAL,and SEL(but not ECL)decreased GFP *** mice with HSV1 keratitis,SAL reduced corneal lesions by 71%.CONCLUSION:The results of this study demonstrate that SAL can be used to inhibit HSV1 infection,particularly *** studies are needed to determine the active components and mechanism underlying the effects of SAL.

关键词： herpes simplex virus type-1 Staphylococcus aureus infection cornea epithelial cells keratitis

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