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Improvement of Two-Dimensional Gel Electrophoresis for Study of corm Formation Related Proteins in vitro from Taro (Colocasia esculenta)

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Journal of Shanghai Jiaotong university(Science) 2005年第S1期10卷 14-18页

作者：杜红梅唐东梅黄丹枫 School of Agriculture and Biology Shanghai Jiaotong Univ. Shanghai 201101 China

Efficient and reproducible sample preparation prior to 2D-PAGE (two-dimensional polyacrylamide gel electrophoresis) is a critical step in achieving accurate and reliable data. In this paper, we described a method to prepare protein samples of taro that was compatible with subsequent analysis using 2D-PAGE. We compared proteins from shoot basal region from 0 d and 2 d after the beginning of tuberization. By this method we got about (2 000) spots and high reproducibility. Additionally some changes of protein expression were found.

关键词： Colocasia esculenta corm two-dimensional gel electrophoresis sample preparation tuberization in vitro

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Biomass Allocation of Scirpus mariqueter Along an Elevational Gradient in a Salt Marsh of the Yangtse River Estuary

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Acta Botanica Sinica 2001年第2期43卷 178-185页

作者：孙书存蔡永立刘红华东师范大学河口海岸国家重点实验室上海200062 华东师范大学环境科学系上海200062 曲阜师范大学地理系山东曲阜250041

Scirpus mariqueter Tang et Zhang is a typical pioneer plant colonizing the bare beaches of the Yangtse River estuary. To explore the life history strategy of the species with reference to environmental physical stress, the biomass allocations to different plant components and some related morphological parameters were examined along an elevational gradient within a salt marsh. Authors found that S. mariqueter performed best at medium elevation within the marsh, with relatively high density of shoot and individual ramet dry mass. Biomass allocation to corm was the highest at low elevations, and the least at high elevations, suggesting that a conservative strategy was adopted by the species to cope with the harsh physical conditions at the low elevation. The investment in rhizome decreased from low to high elevations, while the proportion of inflorescence mass increased, indicating that during the life history, the species shifts from predominant asexual reproduction to predominant sexual reproduction. This may be favourable for the species to colonize larger area, and to spread and persist at a meta_population level. Correlation analyses showed that sexual reproduction was inversely related to growth and asexual reproduction. However, it is difficult to determine the relationship between asexual reproduction and growth possibly because of the varied function of the corms of the species in different life history stages.

关键词： asexual reproduction corm salt marsh sexual reproduction life history strategy trade_off

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In Vitro Regeneration of Endangered Medicinal Hypoxis Species

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American Journal of Plant Sciences 2015年第16期6卷 2585-2595页

作者： Busie E. B. Nsibande Xueyuan Li Annelie Ahlman Li-Hua Zhu Department of Plant Breeding Swedish Agricultural University Alnarp Sweden National Plant Genetic Resources Centre and Mushroom Development Unit Malkerns Swaziland

The genus Hypoxis contains some of southern Africa’s most important wild medicinal species, a situation that has placed most of them amongst endangered species facing extinction. In this study, four Hypoxis species collected from Swaziland: H. argentea, H. filiformis, H. acuminata and H. hemerocallidea, were included to assess their potential for in vitro propagation in order to efficiently conserve these species in the future. Among all types of explants tested only corm explants and seeds gave rise to shoots under the in vitro conditions. For the corm establishment, H. filiformis was the most in vitro responsive species, which had 100% shoot regeneration with high shoot number when cultured on the MS medium supplemented with 3 mg·l-1 kinetin with a piece of shoot attached, while the highest mean shoot number (17) was obtained on the same medium, but with no shoot attachment to the corm explants. H. argentea was the next most responsive species in vitro with up to 70% establishment, while the other two species had only up to 20% of in vitro establishment. For the seed explants, up to 29% seed germination was obtained for H. argentea when the seed coats were crushed before the in vitro culture. The regenerated shoots were rooted in vitro and acclimatized in the greenhouse

关键词： corm Seed Micropropagation Plant Growth Regulators

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Influence of storage life and variety on the micro-nutrient compositions of stored cocoyam-based products

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Food Therapy and Health Care 2022年第2期4卷 10-21页

作者： NP David-Chukwu AG Amadi IC Onuabuchi MN Chukwu Department of Hospitality Management and Tourism Abia State PolytechnicAbaNigeria Department of Food Technology Abia State PolytechnicAbaAbia StateNigeria.

The micronutrient compositions of stored cocoyam-based products were determined using gas chromatography and atomic absorption *** dried cormels and dried cocoyam leaves were pulverized with a locally fabricated machine and stored in various plastic containers for 0,1,2,3 *** data obtained were analyzed statistically using SPSS version *** were separated at P≤0.05 using Fisher’s Least Significant *** vitamin contents(mg/100g)of achicha were as follows:cocoindia had total carotene(1.84),B1(0.071),B2(0.044),B3(0.41),C(4.14)while edeofe had vitamin E(0.84)and total carotene(1.24),B1(0.021),B2(0.013),B3(0.18),C(2.47)and E(0.57)after *** vitamin contents(mg/100g)of mpoto were as follows:cocoindia had B1(0.53),B2(0.31),B3(1.75),C(18.46)and E(5.28)while edeofe had total carotene(3.44μg/100g)and B1(0.26),B2(0.14),B3(1.19),C(8.61)and E(2.46),and anampu had total carotene(2.56μg/100g)after *** mineral composition(mg/100g)of achicha are as follows:anampu had Ca(48.78),Na(18.52),Mg(10.18),Mn(0.17)and P(19.61),Fe(2.47),Zn(2.60),I2(51.71);edeofe had P(26.27),Fe(3.66),Zn(2.91),and Ca(40.53),Na(10.67),Mg(6.29),K(285.77),Mn(0.075);and cocoindia had K(405.87)and I2(69.34)after *** mineral compositions of mpoto(mg/100g)are as follows:anampu had Ca(157.57),Mg(95.84),K(485.52),P(45.52),I2(90.33)and Zn(2.61);edeofe had Fe(4.87),Zn(4.52),and Ca(152.29),Na(176.54),Mg(84.77),K(325.87),P(35.53),Mn(0.25),I2(65.29)and cocoindia had Mg(95.84),Mn(0.63)and Fe(3.61)after *** of the products reduced significantly(P≤0.05)during *** Colocasia products with micronutrients will meet the required recommended dietary intake.

关键词： achicha mpoto corm cormel edeofe cocoindia minerals vitamins

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Exogenous carbon monoxide produces rapid antidepressant-and anxiolytic-like effects

Exogenous carbon monoxide produces rapid antidepressant-and ...

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2019中国生理学会学术年会暨张锡钧基金第十五届全国青年优秀生理学学术论文交流会及第十三届全国青年生理学工作者学术会议

作者： Rafi Ullah Xidi Ma Yipu Zhang Yuan Gao Li Song 史海水 Neuroscience Research Center Hebei Medical University Department of Biochemistry and Molecular Biology College of Basic MedicineHebei Medical University

Carbon monoxide(CO), one of the main products of heme oxygenase-1(HO-1), has been reported to exert significantly protective effects in various neuropathological processes by attenuating inflammatory response and/or promoting ***, the precise roles of CO in depression are still *** hydrogen peroxide(H2 O2)-induced PC12 cell injury model and LPS-induced depression mice model, combined with adeno-associated virus(AAV)-mediated overexpression of HO-1, systemic CO-releasing molecule(corm), CO-rich saline or CO gas treatment procedures, the present study aimed to investigate the potential antidepressant-and anxiolytic-like effects of endogenous and exogenous CO administration in vivo and in *** result showed that AAV-mediated HO-1 overexpression in dorsal hippocampus produced significant antidepressant-and anxiolytic-like activities in mice under normal *** vitro experimental results showed that both corm-3 and corm-A1 pretreatment could block H2 O2-induced cellular injuries, reflected by increased cellular survival, decreased cellular apoptosis and *** to overexpression of HO-1, systemic pretreatment with corm-3, CO gas or CO-rich saline could also produce significant antidepressant-and anxiolytic-like activities in ***, systemic COgas pretreatment could significantly block lipopolysaccharide(LPS)-induced depression-and anxiety-like behaviors in ***, our present findings provide direct evidence, for the first time, that activation of HO-1 and/or exogenous CO administration could produce protective effects and exert significant antidepressant-and anxiolytic-lieke effects, highlighting that the HO-1/CO system, especially CO application could be a promising therapeutic target for treatment of depression and anxiety.

关键词： Carbon monoxide Heme oxygenase-1 Depression Anxiety corm

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新型含双苯基环丙烯酮和查尔酮聚合物设计合成及其力致响应性

新型含双苯基环丙烯酮和查尔酮聚合物设计合成及其力致响应性

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作者：邵琛琛浙江大学

学位级别：硕士

高分子力化学作为一个历久弥新的研究领域,在力的作用下,分子链经历从“破坏”到“构建”的转变,而力敏基团是转变中的关键因素。许多力敏基团被设计出来并呈现不同的应用.。本文分别设计了链中心埋置双苯基环丙烯酮和查尔酮二聚体的聚... 详细信息

高分子力化学作为一个历久弥新的研究领域,在力的作用下,分子链经历从“破坏”到“构建”的转变,而力敏基团是转变中的关键因素。许多力敏基团被设计出来并呈现不同的应用.。本文分别设计了链中心埋置双苯基环丙烯酮和查尔酮二聚体的聚合物,探索研究了这些聚合物的力化学反应行为。一、链中心含双苯基环丙烯酮基的聚合物的合成和双重响应。将含酯键化的双苯基环丙烯酮引入线型聚合物链中心位置,在稀溶液状态下探究了其超声响应和光响应的行为。通过动力学和和核磁结果分析发现,该聚合物在超声作用下链中央的酯键发生断裂,双苯基环丙烯酮基团并未发生化学变化;发现了溶剂中的微量水对酯键断裂起到促进作用并有上限,提出了力致芳香酯键水解的反应类型。同时,该聚合物在紫外光辐照下,可以“开关”方式释放一氧化碳(CO)并形成双苯基乙炔结构。由此提供了一种不含金属的可降解释放一氧化碳大分子(corm)模型。同时研究了将双苯基环丙烯酮引入交联聚氨酯中的本体双重响应性。发现在紫外光照下该聚氨酯可以释放CO,提供了可本体释放CO的新体系。二、链中心为查尔酮基的聚合物的合成及力响应行为。首先将查尔酮与丙烯酸酯类共聚,制备了含查尔酮二聚体的聚合物,制成聚合物凝胶,探究了其在超声下的稳定性。经过核磁和凝胶渗透色谱(GPC)结果对比发现,交联的聚合物凝胶在超声下发生解聚,产生含查尔酮侧基的线型链。初步证明了四元环结构的查尔酮二聚体是力敏基团。同时合成了三种不同结构的查尔酮二聚体,设计了三种查尔酮二聚体的力响应体系以用于探究力致区域选择性开环和电子效应对其力响应的影响。综上所述,本文设计了新的含双苯基环丙烯酮和查尔酮二聚体的聚合物,探究了它们的力致响应性,获得了新的力敏基团,研究了这些聚合物的响应行为,深入研究了部分体系的响应机理。

关键词：力敏基团双苯基环丙烯酮 corm 查尔酮

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