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The SNW domain of SKIP Is Required for Its Integration into the Spliceosome and Its Interaction with the Pall Complex in Arabidopsis

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Molecular Plant 2016年第7期9卷 1040-1050页

作者： Yan Li Con qconcl Xia Jinlin Feng Dong Yang Fangming Wu Ying Cao Legong Li Ligeng Ma College of Life Sciences Hebei Normal University Shijiazhuang Hebei 050021 China Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement Beijing Municipal GovernmentBeijing 100048 China College of Life Sciences Capital Normal University Beijing 100048 China

SKIP is a conserved protein from yeasts to plants and humans. In plant cells, SKIP is a bifunctional regulator that works in the nucleus as a splicing factor by integrating into the spliceosome and as a transcriptional activator by interacting with the Pall complex. In this study, we identified two nuclear localization signals in SKIP and confirmed that each is sufficient to target SKIP to the nucleus. The SNW domain of SKIP is required for both its function as a splicing factor by promoting integration into the spliceosome in response to stress, and its function as a transcriptional activator by controlling its interaction with the Pall complex to participate in flowering. Truncated proteins that included the SNW domain and the N- or C-terminus of SKIP were still able to carry out the functions of the full-length protein in gene splicing and transcriptional activation in Arabidopsis. In addition, we found that SKIP undergoes 26S proteasome-mediated degrada- tion, and that the C-terminus of SKIP is required to maintain the stability of the protein in plant cells. Together, our findings demonstrate the structural domain organization of SKIP and reveal the core domains and motifs underlying SKIP function in plants.

关键词： SKIP spliceosome Pafl complex core domain and motif nuclear localization signal 26Sproteasome-mediated degradation

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