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Light and Heat Induced Denaturation of Photosystem Ⅱ core antenna Complex CP47

Tsinghua Science and Technology 1999年第3期4卷 1485-1488页

作者：王居硕单际修许强公衍道匡廷云赵南明 Department of Biological Sciences and Biotechnology State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing 100084 Photosynthesis Research Center Institute of Botany Chinese Academy of Sciences Beijing 100093

Light and heat induced denaturation of CP47, the core antenna complex of photosystem Ⅱ purified from spinach, were investigated using absorption and circular dichroism spectra. Light caused the destruction of chlorophyll a and excitonic interaction of chlorophyll a in CP47, while the protein secondary structure was not apparently changed. Heat induced the destruction of protein secondary structure and excitonic interaction of chlorophyll a, but the chlorophyll a molecule was not damaged. The results suggest that both the chlorophyll a molecular structure and the protein native conformation are necessary for excitonic interaction of chlorophyll a and the energy transfer function of the chlorophyll a binding protein.

关键词： photosystem Ⅱ core antenna CP47 circular dichroism

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Kinetic Fluorescence Spectral Analysis of core antennas CP43 and CP47 of Photosystem Ⅱ with Ultrafast Time-resolved Technology

引用

Acta Botanica Sinica 2001年第7期43卷 704-708页

作者：贺俊芳王水才张舒贺锋涛单际修匡廷云中国科学院西安光学精密机械研究所瞬态光学技术国家重点实验室西安710068 中国科学院植物研究所光合作用基础研究开放实验室北京100093

Ultrafast time_resolved fluorescence experiments have been performed with core antennas CP43 and CP47 of PS Ⅱ. Their dynamic fluorescence spectra were obtained with excitation wavelength 514.5 nm. For CP43, the emission spectrum was found to be from 640 to 780 nm with a peak at ～680 nm and the lifetime of fluorescence was 3.54 ns. For CP47, the emission spectrum was from 630 to 775 nm with a peak at ～691 nm and the fluorescence lifetime was 3.22 ns. The fluorescence emission efficiencies of Chl a in CP43 and CP47 were calculated to be approximately 38.3% and 40.6%, respectively. The energy transfer from β_Car to Chl a in CP43 and CP47 was discussed. The rates of energy transfer from β_Car to Chl a were measured to be about 9.6×10 11 s -1 and 1.3×10 12 s -1 and energy transfer efficiencies 47.5% and 66.5% respectively. The edge_edge distances between β_Car and Chl a in CP43 and CP47 were estimated to be ～0.110 nm and ～0.085 nm respectively.

关键词： core antenna CP43 CP47 β-Car Chl a energy transfer PS Ⅱ

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