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An analysis of silver candlesticks from a casting point of view:originals,copies,forgeries

China Foundry 2010年第1期7卷 68-75页

作者： Jaromir Audy Emil Evin Faculty of Regional Professional Studies School of Enterprise and TechnologyEdith Cowan UniversitySouth West Campus BunburyAustralia 6230 Department Technologies and Materials Faculty of Mechanical EngineeringTechnical University KosiceM-siarska 74Ko-iceSlovakia 04001

Collecting silver artefacts has traditionally been a very popular *** is addictive,therefore the number of potential collectors and investors appears to grow each ***,increases in the interest and buying potentials resulted in a number of forgeries manufactured and introduced to the open antique *** items such as early silver candlesticks dictate a very high price,for many high quality fakes show very good appearances and matching similarities with *** copies are traditionally manufactured by casting using the original items as *** details and variances in design features,position and shape of hallmarks,including the final surface quality are usual features to distinguish the fakes from the *** paper presents results of a study conducted on several silver candlesticks,including two artefacts bearing features of those produced in the mid 18th century,one original Italian candelabrum from Fascist era,and small candlesticks made in the early 20th ***,the paper presents some interesting contemporary coins-replicas of many those produced in different *** coins were offered for sale by unscrupulous dealers via auctions and *** the main results and findings from this study are discussed from a manufacturing point of view,such as fabrication technology,surface quality and hallmarks,which will help the collectors,dealers and investors to detect and avoid forgeries.

关键词： candlesticks candelabra originals copies forgeries silver manufacturing technology hallmarks

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Detection of Exogenous Gene copies in Transgenic Soybean by Taqman Quantitative PCR Technique

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Agricultural Science & Technology 2011年第4期12卷 497-499,553页

作者：仇有文张明辉高学军曲波敖金霞袁肖寒刘营霍楠东北农业大学生命科学与生物技术研究中心农业部转基因生物产品成分监督检测测试中心黑龙江哈尔滨150030 东北农业大学生命科学与生物技术研究中心农农业部转基因生物产品成分监督检测测试中心黑龙江哈尔滨150030

[Objective] Taqman Quantitative PCR technique was adopted to detect the copies of exogenous nos terminator in transgenic hybrid soybean.[Method] With soybean Lectin as the endogenous reference gene,and gene complex DNA in non-GMO soybeans as the endogenous reference standard,the method of gradient dilution was used for separately calculate Ct value of endogenous reference gene and plasmid DNA and correlation standard curve equation of logarithm of copies,and then to calculate the copies of samples through substituting thus-obtained Ct into the standard curve equation.[Result] The standard curve equation of endogenous reference gene is y=-3.422x＋35.201,R2=0.998;and the standard curve equation of exogenous gene is y=-3.348x＋34.890,R2=*** terminator and its lower boundary sequences in transgenic soybean is of single copy.[Conclusion] The study has provided a theoretical basis for determining exogenous gene copies in transgenic soybean.

关键词： Real-time PCR Transgenic hybrid soybean copies Lectin Boundary sequence of nos terminator gene

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Human embryonic stem cell lines with ccr5-del32 allele conferring resistance to HIV

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Stem Cell Discovery 2011年第3期1卷 67-70页

作者： Ekaterina Pomerantseva Valeri Kukharenko Adam Goodman Oleg Verlinsky Svetlana Rechitsky Anver Kuliev 不详

A 32bp deletion in the chemokine receptor 5 (CCR5) gene (CMKBR5) was shown to be linked to HIV resistance. Bone marrow transplantation from the homozygous CCR5-del32 donor to a CDC Stage 2 HIV-positive recipient was demonstrated to confer a HIV resistance, resulting in discontinuation of antiretroviral therapy. In search for an unlimited source of CCR5-del32 cells for transplantation purposes, we tested 137 human embryonic stem cell (hESC) lines from the Reproductive Genetics Institute’s hESC lines collection, and report here the finding of 12 hESC lines with the CCR5-del32 allele, one of which represents a unique partenogenetic ESC line containing two copies of this deletion and may be studied for utility in stem cell transplantation treatment of HIV.

关键词： Human embryonic stem cell lines Resistance to HIV CCR5-del32 allele Parthenogenetic stem cell line with two copies of CCR5-del32 allele Stem cells transplantation

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