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High production of fatty alcohols in Yarrowia lipolytica by coordination with glycolysis

Science China Chemistry 2019年第8期62卷 1007-1016页

作者： Jin-Lai Zhang Ying-Xiu Cao Yang-Zi Peng Cong-Cong Jin Qiu-Yan Bai Ruo-Si Zhang Duo Liu Ying-Jin Yuan Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education) Tianjin University Tianjin 300072China Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) School of Chemical Engineering and Technology Tianjin UniversityTianjin 300072China

Fatty alcohol biosynthesis by oleaginous microbes was a promising alternative to the petroleum or other non-renewable resources-based process. However, low titer and yield hamper the further industrial and commercial applications. Here, we developed an efficient strategy to coordinate fatty alcohol with glycolysis which achieved a ‘pull-and-push’ effect to improve fatty alcohol production. Transcript profiling indicated that genes in carbohydrate metabolism were up-regulated significantly in response to high fatty alcohol production. Based on it, 11 glycolysis promoters were screening to express fatty acyl-CoA reductase(FAR) to relate the fatty alcohol production with the up-regulated carbohydrate metabolism, and the fatty alcohol production reached 557 mg/L when FAR was expressed by the promoter of PFBAin. RNA-seq and qRT-PCR analysis demonstrated that a ‘pull-and-push’ effect caused by the coordination system dynamically enhanced the product synthesis flux from top to bottom, which was also testified and intensified by doubled glucose concentration. After manipulating structural and regulatory genes of lipid metabolism, the final strain achieved up to 5.75 g/L fatty alcohol production from modified YPD medium(containing 91 g/L glucose) in shake flasks, which represented the highest titer reported to date. This work offered a feasible and effective reference for dynamic manipulation of fatty acid-derived chemicals synthesis.

关键词： fatty alcohol biosynthesis coordinative expression Yarrowia lipolytica fatty acyl-CoA reductase(FAR) metabolic engineering

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Growth Units and Formation Mechanisms of the Crystals Under Hydrothermal Conditions

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Science China Chemistry 1994年第11期37卷 1288-1297页

作者：仲维卓刘光照施尔畏华素坤唐鼎元赵庆兰 Shanghai Institute of Ceramics Academia SinicaShanghai 200050PRC Fujian Institute of Research of the Structure of Matter Academia SinicaFuzhou 350002PRC Fujian Institute of Research on the Structure of Matter Academia SinicaFuzhou 350002PRC

The growth units and formation mechanisms of quartz(SiO_2)and barium titanate (BaTiO_3)crystals under hydrothermal conditions have been investigated by means of cataphoretic experi- *** is proposed that the growth units of the crystals in hydrothermal solutions have the structures of coordinative polyhedra of anions,which correspond to those of coordinative polyhedra in the crystals. The relationship between crystal morphology and physico-chemical conditions has been explained accord- ing to the modes of incorporation of the coordinative polyhedra of anions on the various faces of crystals. The dimensions of the growth units vary with the change of the growth *** incorporation rates on the various faces of the growth units with varied dimensions must be different,which leads to a satis- factory explanation for the variety of the crystal morphology.

关键词： growth unit coordinative polyhedra of anion crystallization habit

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Recovery of heavy metals from electroplating sludge and stainless steel pickle waste liquid by ammonia leaching method

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Journal of Environmental Sciences 1999年第3期11卷 381-384页

作者： Zhang Yi, Wang Zhi\|kuan, Xu Xia, Chen Yong\|qi, Qi Tao Institute of Chemical Metallurgy, Chinese Academy of Sciences,P.O. Box 353, Beijing 100080, China 中科院化工冶金所北京 100080 中科院化工冶金所北京

A coordinative disposal process for treatment of electroplating sludge and stainless steel pickle waste liquid containing Cu, Ni, Zn, Cr and Fe etc., has been developed to recover valuable metals and to eliminate pollution. The recovery of Cu, Ni, Zn and Cr is 94%, 91%, 90% and 95%, respectively. The ammonia was recycled by the simplified process of CaO caustic distillation. The precipitated product of Cu, Ni and Zn obtained from caustic distillation of ammonia was separated by extraction or high\|pressure hydrogen reduction in an autoclave. The qualified metal salt products were obtained through extraction. The rich chromium residue from coordinative disposal was subjected to recover Cr by hydrothermal oxidation in NaOH medium and Fe\-3O\-4 was synthesized by wet methods from the residue produced by extracting Cr. Cr was a stable chemical fixed in Fe\-3O\-4 and harmless. The recovery process has been used in a pilot plant with sludge production capacity of 2000 t/a.

关键词： coordinative disposal recovery valuable metals electroplating sludge stainless steel pickle waste liquid.

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