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conjugated polyelectrolyte-Ag＋ Fluorescent Switch for Biothiol Sensing

Chemical Research in Chinese Universities 2013年第5期29卷 845-849页

作者： XIAO Yi LI Sha HUANG Hong-mei ZHANG Ye XUE Zun-zun TANG Xue-yan HE Xiao-xiao WANG Ke-min Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministry of Education College of Chemistry and Chemical Engineering Hunan Normal University Changsha 410081 P. R. China State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering College of Biology Hunan University Changsha 410082 P. R. China

A new ＂off-on＂ switch for sensitive and selective fluorescence detection of biothiols[glutathione（GSH）, cysteine（Cys） and homocysteine（Hcy）] was developed based on an anionic conjugated polyelectrolyte（CPE）, pyridyl-functionalized poly（phenylene ethynylene）（Pl）. The fluorescence of P1 can be significantly quenched by Ag＋ due to complexation-mediated interpolymer aggregation. Furthermore, biothiols can efficiently recover the fluorescence intensity of P1 as a result of the stronger binding between thiol group and Ag＋, which dissociates PI from the P1/Ag＋ complex and disrupts interpolymer aggregation. Under optimum conditions, a good linear relation- ship in a range of 100--4200 nmol/L is obtained for GSH with a detection limit of 80 nmol/L（S/N=3）. As a result of specific interaction between the thiol group and Ag＋, the proposed method shows a high selectivity for biothiols. In addition, the CPE-based fluorescence ＂off-on＂ switch has been used to quantitatively detect total biothiols in cell lysates.

关键词： Fluorescent switch Biothiol Silver(I) conjugated polyelectrolyte

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White Light-induced Cell Apoptosis of a conjugated polyelectrolyte with Singlet Oxygen Generation

White Light-induced Cell Apoptosis of a Conjugated Polyelect...

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第十届全国化学生物学学术会议

作者： Jiamei Liang Pan Wu Chunyan Tan Department of Chemistry Tsinghua University Shenzhen Key Laboratory of Chemical Biology the Graduate School at ShenzhenTsinghua University

Photodynamic therapy(PDT) sensitizer, which generates cytotoxic singlet oxygen(O) under irradiation, has attracted considerable attention for its significant applications in cancer therapy as well as the participation of O in the regulation of cell death pathways[1]. conjugated polyelectrolytes(CPEs) consisting delocalized electronic backbone with ionic side chains exhibit good cell compatibility, strong light-harvesting ability and excellent photostability. Some CPEs have become a promising sensitizers in cancer cell ablation because of their efficient photosensitization property[2]. In our previous work[3],[4], we synthesized cationic poly(p-phenylene ethynylene terthiophene)(PPET3-N+) which generated reactive oxygen species(ROS) under white light. In this work, we investigated the efficacy of PPET3-N+ in white light-induce PDT treatment of Hela cells and the mechanisms of cell death. PPET3-N+ showed efficient singlet oxygen generation and exhibited low cytotoxicity in dark as well as an increase in photocytotoxicity with increasing PDT doses(sensitizer dose and light dose). The mechanisms of cell apoptosis induced by PPET3-N+ PDT have been evaluated through various methods: flow cytometric analysis of apoptosis cells, mitochondrial membrane potential(MMP) measurement and western blot. Based on the results, we concluded that PPET3-N+ induce mitochondrial apoptosis under white light irradiation.

关键词： conjugated polyelectrolyte Singlet Oxygen Cell Apoptosis

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conjugated polyelectrolytes for label-free visual detection of heparin

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Science China Chemistry 2011年第4期54卷 567-574页

作者： PU KanYi ZHAN RuoYu LIANG Jing LIU Bin Department of Chemical and Biomolecular Engineering National University of SingaporeSingapore 117576Singapore

conjugated polyelectrolytes (CPEs),with electron-delocalized backbones and charged ionic side chains,are unique sensory materials for the construction of optical *** this feature article,CPE-based visual detection of heparin is *** CPE-based heparin probes,including ratiometric,light-up and colormetric probes,are discussed in terms of molecular design,detection selectivity and quantification *** work summarized here provides fundamental guidelines for CPE-based label-free visual sensing.

关键词： conjugated polyelectrolyte heparin fluorescence resonance energy transfer sensor

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