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Trichinella spiralis calreticulin inhibits the complement classical pathway activation by interaction with human complement C1q

Trichinella spiralis calreticulin inhibits the complement cl...

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中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会

作者： Limei Zhao Shuai Shao Yi Chen Jing Yang Xinping Zhu Department of Parasitology School of Basic Medical Sciences Capital Medical University

Objective To explore the role of Trichinella spiralis calreticulin(Ts-CRT) in host immune attack by interference with the complement function, therefore deeply clarifying the mechanisms of *** evading from complement killing Methods Recombinant Ts-CRT(r Ts-CRT) was expressed and its ability to bind calcium was assessed using "Stains-all". The expression pattern and distribution of Ts-CRT were detected by Western blot and confocal immunofluorescence. The binding between r Ts-CRT and C1 q was determined by ELISA, Far Western blot and co-immunoprecipitation. The activation products of C4/C3 deposition were detected with ELISA and the complement-mediated lysis of sheep erythrocytes was investigated with hemolysis assay. The effect of r Ts-CRT on C1 q interacting with its receptor on THP-1-derived macrophages was examined by cell immunofluorescence staining and the C1q-induced chemotaxis of macrophages was determined by Transwell. Results The results of "Stains-all" suggested that r Ts-CRT had the ability to bind Ca2+. ELIAS and expressed all the developmental stages. Far Western blot and co-immunoprecipitation experiments showed that r Ts-CRT was able to bind human C1 q. The interaction of rT s-CRT and C1 q significantly reduced C4 and C3 deposition to the activator Ig M. The C1q-dependent lysis of sensitized sheep erythrocytes was also inhibited by r Ts-CRT. rT s-CRT also inhibited C1 q interacting with its receptor on THP-1-derived M2 phenotype macrophage and suppressed C1q-induced macrophages chemotaxis. Conclusion These findings suggest that Ts-CRT plays an important role in the immune evasion by inhibiting the initial stage of host complement activation.

关键词： Trichinella spiralis calrecitulin complement C1q immune evasion complement attack

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