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Effect of cO2 pneumoperitoneum on the expression of the chemokine receptors cXcR4 and ccR7 in colorectal carcinoma cells in vitro

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chinese Medical Journal 2013年第24期126卷 4747-4751页

作者： YANG chun-kang LI Guo-dong Y1NG Min-gang XU Ke Department of Abdominal Surgery Fujian Provincial Cancer Hospital Fuzhou Fujian 350014 China Department of Oncology Guizhou Cancer Hospital GuiyangGuizhou 550004 China Provincial clinical coliege Fujian Medical University FuzhouFujian 350014 China

Background The ability of pneumoperitoneum in laparoscopic surgery to promote proliferation and metastasis of colorectal cancer has become a focus of research in the field of minimally invasive surgery. The aim of this research was to investigate the effect of cO~ pneumoperitoneum under different pressures and exposed times on the expression of chemokine receptors in colorectal carcinoma cells. Methods We constructed an in vitro pneumoperitoneum model. SW480 colon carcinoma cells were exposed to cO2 pneumoperitoneum under different pressures （6, 9, 12, and 15 mmHg） for 1, 2, and 4 hours. These cells were then cultivated under the same conditions as normal SW480 colon carcinoma cells without cO= pneumoperitoneum （control group）, treated at 37℃, and 5% cO2. The expression of the chemokine receptors cXc receptor 4 （cXcR4） and chemokine c receptor 7 （ccR7） was detected by immunocytochemistry and reverse transcriptase polymerase chain reaction after being cultivated for 0, 24, 48, and 72 hours. Results Immunocytochemistry showed that cXcR4 expression in SW480 cells was significantly decreased in the 6, 9, 12, and 15 mmHg cO2 pneumoperitoneum-treated groups for the same exposure times compared with controls （P 〈0.05）. ccR7 expression in SW480 cells was significantly decreased in the 12 and 15 mmHg cO2 pneumoperitoneum- treated groups compared with controls （P 〈0.05）. cXcR4 and ccR7 expression increased up to the level of the control group after 24 and 48 hours （P 〉0.05）. If the cO2 pneumoperitoneum pressure increased, cXcR4 and ccR7 expression decreased at all exposure times. If the cO2 pneumoperitoneum exposure time prolonged, there were no significant differences in cXcR4 and ccR7 expression under the same pressure. Under all exposure times, cXcR4 and ccR7 mRNA expression was significantly decreased in the 6, 9, 12, and 15 mmHg cO2 pneumoperitoneum-treated groups （P 〈0.05） compared with controls, and it increased up to the level of controls after being cultivated for 48

关键词： colorectal neoplasm, c02 pneumoperitoneum chemokine receptor, cXc receptor 4, chemokine c receptor 7

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