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Neuronal-like cell differentiation of non-adherent bone marrow cell-derived mesenchymal stem cells

Neural Regeneration Research 2013年第22期8卷 2078-2085页

作者： Yuxin Wu Jinghan Zhang Xiaoming Ben Department of Neurosurgery Nanjing Children's Hospital of Nanjing Medical University Department of Newborn Medicine Nanjing Children's Hospital of Nanjing Medical University Department of Newborn Medicine Shanghai First Maternity and Infant HospitalTongji University School of Medicine

Non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL/6J mice were sepa- rated and cultured using the ＂pour-off＂ method. Non-adherent bone marrow cell-derived mesen- chymal stem ceils developed colony-forming unit-fibroblasts, and could be expanded by supple- mentation with epidermal growth factor. Immunocytochemistry showed that the non-adherent bone marrow cell-derived mesenchymal stem cells exposed to basic fibroblast growth factor/epidermal growth factor/nerve growth factor expressed the neuron specific markers, neurofilament-200 and NeuN, in vitro. Non-adherent bone marrow cell-derived mesenchymal stem cells from 13-galactosidase transgenic mice were also transplanted into focal ischemic brain （right corpus striatum） of C57BL/6J mice. At 8 weeks, cells positive for LacZ and 13-galactosidase staining were observed in the ischemic tissues, and cells co-labeled with both 13-galactosidase and NeuN were seen by double immunohistochemical staining. These findings suggest that the non-adherent bone marrow cell-derived mesenchymal stem cells could differentiate into neuronal-like cells in vitro and in vivo.

关键词： neural regeneration stem cells non-adherent bone marrow cell-derived mesenchymal stem cells neuronal-like cells colony-forming unit-fibroblasts proliferation differentiation beta-galactosidasetransgenic mouse cell transplantation cerebral ischemia bone marrow cells-derived mesenchymalstem cells grants-supported paper neuroregeneration

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