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Heterologous expression of the ThIPK2 gene enhances drought resistance of common wheat

Journal of Integrative Agriculture 2020年第4期19卷 941-952页

作者： ZHANG Shu-juan LI Yu-lian SONG Guo-qi GAO Jie ZHANG Rong-zhi LI Wei CHEN Ming-li LI Gen-ying Crop Research Institute Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River ValleyMinistry of Agriculture/National Engineering Laboratory for Wheat and MaizeJinan 250100P.R.China

Th IPK2 is an inositol polyphosphate kinase gene cloned from Thellungiella halophila that participates in diverse cellular processes. Drought is a major limiting factor in wheat(Triticum aestivum L.) production. The present study investigated whether the application of the Th IPK2 gene could increase the drought resistance of transgenic wheat. The codon-optimized Th IPK2 gene was transferred into common wheat through Agrobacterium-mediated transformation driven by either a constitutive maize ubiquitin promoter or a stress-inducible rd29 A promoter from Arabidopsis. Molecular characterization confirmed the presence of the foreign gene in the transformed plants. The transgenic expression of Th IPK2 in wheat led to significantly improve drought tolerance compared to that observed in control plants. Compared to the wild type(WT) plants, the transgenic plants showed higher seed germination rates, better developed root systems, a higher relative water content(RWC) and total soluble sugar content, and less cell membrane damage under drought stress conditions. The expression profiles showed different expression patterns with the use of different promoters. The codon-optimized Th IPK2 gene is a candidate gene to enhance wheat drought stress tolerance by genetic engineering.

关键词： codon-optimized drought rd29A ThIPK2 Triticum aestivum L.

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Heterologous expression of the ThIPK2 gene enhances drought resistance of common wheat

Heterologous expression of the ThIPK2 gene enhances drought ...

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中国生物工程学会第十三届学术年会暨2019年全国生物技术大会

作者： ZHANG Shu-juan LI Yu-lian SONG Guo-qi GAO Jie ZHANG Rong-zhi LI Wei CHEN Ming-li LI Gen-ying Crop Research Institute Shandong Academy of Agricultural Science/Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley Ministry of Agriculture/National Engineering Laboratory for Wheat and Maize

ThIPK2 is an inositol polyphosphate kinase gene cloned from Thellungiella halophila that participates in diverse cellular processes. Drought is a major limiting factor in wheat(Triticum aestivum L.) production. The present study investigated whether the application of the Th IPK2 gene could increase the drought resistance of transgenic wheat. The codon-optimized Th IPK2 gene was transferred into common wheat through Agrobacterium-mediated transformation driven by either a constitutive maize ubiquitin promoter or a stress-inducible rd29 A promoter from Arabidopsis. Molecular characterization confirmed the presence of the foreign gene in the transformed plants. The transgenic expression of Th IPK2 in wheat led to significantly improved drought tolerance compared to that observed in control plants. Compared to the WT plants, the transgenic plants showed higher seed germination rates, better developed root systems, a higher relative water content(RWC) and total soluble sugar content, and less cell membrane damage under drought stress conditions. The expression profiles showed different expression patterns with the use of different promoters. The codon-optimized Th IPK2 gene is a candidate gene to enhance wheat drought stress tolerance by genetic engineering.

关键词： codon-optimized drought rd29A ThIPK2 Triticum aestivum L

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