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Meta-analysis of the cell cycle related C12orf48

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BIOCELL 2013年第1期37卷 11-16页

作者： LOKMAN VARISLI Harran University Art and Science FacultyDepartment of BiologyOsmanbey CampusSanliurfaTurkey Harran UniversityArt and Science FacultyDepartment of BiologyOsmanbey CampusSanliurfaTurkey

The cell cycle is a conserved process from yeast to mammals and focuses on mechanisms thatregulate the timing and frequency of DNA replication and cell division.The temporal and spatial expressionof the genes is tightly regulated to ensure accurate replication and transmission of DNA to daughter cellsduring the cycle.Although the genes involved in interphase are well studied,most of the genes which areinvolved in mitotic events still remain unidentified.Since,the discovery of mitosis related genes is stillincomplete,we performed a co-expression and gene ontology analysis for revealing novel mitosis regulatedgenes.In this study,we showed that C12orf48 is co-expressed with well-known mitotic genes.Moreover,it isalso co-expressed with the genes that have roles in interphase such as DNA replication.Furthermore,ourresults showed that C12orf48 is also differentially expressed in various cancers.Therefore,the results pre-sented in this study suggest that C12orf48 may be an important molecule for both interphase and mitosis.Since,the molecules involved in these mechanisms are crucial for proliferation as well as in carcinogenesis,C12orf48 should be considered as a novel cell cycle and carcinogenesis related gene.

关键词： C12orf48 cell cycle co-expression gene ontology oncomine

Unravelling lncRNA mediated gene expression as potential mechanism for regulating secondary metabolism in Citrus limon

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Food Bioscience 2022年第2期46卷 703-718页

作者： Kuntala Sarma Bordoloi Pooja Moni Baruah Mami Das Niraj Agarwala Department of Botany Gauhati UniversityGopinath Bordoloi NagarGuwahatiAssam781014India

Plant long non-coding RNAs(lncRNAs)are emerging as important regulators of gene expression through various mechanisms viz.binding to double-stranded DNA(dsDNA),physically targeting mRNAs and also act as microRNA(miRNA)sponges.Characteristic secondary metabolites and their content determine the market value of Citrus plant products for culinary and industrial uses.Though lncRNAs are emerging as important molecular players that regulate secondary metabolites in plants but the roles of lncRNAs are yet unknown for the regulation of secondary metabolites in Citrus limon.In this study,a total of 11814 lncRNAs were identified from four RNA-seq datasets comprising of data from four different tissues of C.limon viz.leaf,bud,fruit and peel.co-expression analysis of whole set of genes with identified lncRNAs revealed a total of 632 lncRNAs correlating with 5810 genes.Functional annotation of genes correlating with lncRNAs unveiled the association of 379 genes with the biosynthesis of secondary metabolite pathways and among them,36 and 12 genes are from terpenoids and flavonoids metabolism/biosynthesis pathways respectively.expression correlation analysis unfolded the possible role of 113 lncRNAs in terpenoids metabolism and 29 lncRNAs in the flavonoids metabolic pathway.Seventy nine lncRNAs were predicted to take part in miRNA-mediated gene regulation,out of which 10 lncRNAs were predicted to act as endogenous target mimics(eTMs)for miRNAs and 74 lncRNAs were found to act as miRNA targets.expression of six predicted lncRNAs and seven correlated genes involved in terpenoids and flavonoids metabolism were validated using semi-quantitative RT-PCR.

关键词： Citrus limon Long non-coding RNA Terpenoids Flavonoids co-expression

co-expression of ScBADH and haFGF in transgenic hybrids produces recombinant protein and improves stress tolerance in Safflower(Carthamus tinctorius L.)

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Co-expression of ScBADH and haFGF in transgenic hybrids prod...

2011全国植物生物学研讨会

作者： Yanxi Yuan~1,Weiwei Huo~1,Jing Chen~1,Qiongqiong Li~1,Xiaomei Zhang~1,Nan Wang~2, Xiuming Liu~2,Yanfang Wang~2,Jing Yang~2,Fawei Wang~2,Jian Wei~2,Haiyan Li~(1,2*) 1 college of Life Science,Jilin Agricultural University,Changchun,Jilin 130118,China 2 Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University,Changchun,Jilin 130118,China

Currently,the plant bioreactor has been investigated as a promising production platform of recombinant proteins(particularly human therapeutics) due to their intrinsic safety(they do not propagate mammalian viruses and pathogens), economic-valuable bioprocessing that leads to lower production and downstream costs,and the capability for post-translation modifications.However,with the rapid increasing of world population and the global area of salinized land has expanded each year,which seriously affect the cultivation of food crops production.In this study,in order to produce the human therapeutics recombinant protein-acid fibroblast growth factor(haFGF) in safflower bioreactor by growing in saline-alkali land of Northeast China,safflower were transgenic for both ScBADH(encoding betaineal dehydedehy drogenase from the halophyte Suaeda corniculata) and haFGF(encoding

关键词： co-expression haFGF Safflower ScBADH

co-expression of △5-Elongase and △4-Desaturase in Saccharomyces cerevisiae

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Co-expression of △5-Elongase and △4-Desaturase in Saccharo...

第八届中国酶工程学术研讨会

作者： Jianzhong Huang Xianzhang Jiang Songgang Wu Engineering Research Center of Industrial Microbiology Ministry of EducationFujian Normal University

＜正＞Docosahexaenoic acid（DHA,22:6△4,7,10,13,16,19,n-3）is an essential long chain polyunsaturated fatty acid that plays many beneficial roles in human health[1-3].Cloning the genes encoding for enzymes which involve in the biosynthesis of DHA has recently drawn much scientific attention.DHA-producing strain Thraustochytrium sp.FJN-10 was isolated by using pine pollen as a bait in our laboratory,in its

关键词： Docosahexaenoic acid △5-Elongase △4-Desaturase co-expression

Global identification of genes associated with xylan biosynthesis in cotton fiber

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Journal of cotton Research 2020年第3期3卷 184-198页

作者： CHEN Feng GUO Yanjun CHEN Li GAN Xinli LIU Min LI Juan XU Wenliang Hubei Key Laboratory of Genetic Regulation and Integrative Biology School of Life SciencesCentral China Normal UniversityWuhan 430079China Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou 450001China

Background:Mature cotton fiber secondary cell wall comprises largely of cellulose(>90%)and small amounts of xylan and lignin.Little is known about the cotton fiber xylan biosynthesis by far.Results:To comprehensively survey xylan biosynthetic genes in cotton fiber,we identified five IRX9,five IRX10,one IRX14,six IRX15,two FRA8,one PARVUS,eight GUX,four GXM,two RWA,two AXY9,13 TBL genes by using phylogenetic analysis coupled with expression profile analysis and co-expression analyses.In addition,we also identified two GT61 members,two GT47 members,and two DUF579 family members whose homologs in Arabidopsis were not functionally characterized.These 55 genes were regarded as the most probable genes to be involved in fiber xylan biosynthesis.Further complementation analysis indicated that one IRX10 like and two FRA8 related genes were able to partially recover the irregular xylem phenotype conferred by the xylan deficiency in their respective Arabidopsis mutant.We conclude that these genes are functional orthologs of respective genes that are implicated in GX biosynthesis.conclusion:The list of 55 cotton genes presented here provides not only a solid basis to uncover the biosynthesis of xylan in cotton fiber,but also a genetic resource potentially useful for future studies aiming at fiber improvement via biotechnological approaches.

关键词： cotton fiber Secondary cell wall Xylan biosynthesis expression profile co-expression

co-expression of TRAIL and Smac delivered by an E1B-55K deleted oncolytic adenovirus containing a caspase-8 cleavage site (IETD) elicits complete elimination of hepatocellular carcinoma xenograft

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Co-expression of TRAIL and Smac delivered by an E1B-55K dele...

2012年浙江省血液病学年会

作者： Shibing Wang Yuan Tan Wen Lei Yigang Wang Xiumei Zhou Kangjian Zhang Liang Chu Xinyuan Liu Wenbin Qian Xinyuan Institute of Medicine and Biotechnology School of Life Science Zhejiang Sci-Tech University Institute of Hematology The First Affiliated Hospital College of Medicine Zhejiang University Laboratory of Molecular Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences

combination target therapy is necessary for cancer treatment because tumors are genetically diverse. In our current study, we generated a novel E1B-55K deleted oncolytic adenovirus that harbors both TRAIL and Smac genes (ZD55-TRAIL-IETD-Smac). In this vector, a caspase-8 cleavage site (IETD) was introduced between the genes, which allows the production of TRAIL and Smac, respectively, within virusinfected cells after activation of caspase-8. In our system, treatment with ZD55-TRAIL-IETD-Smac produced equal expression of TRAIL and Smac, and resulted in significant cytotoxicity against various cancer cells with stronger efficacy than that of ZD55-TRAIL or ZD55-Smac alone. The expression of these two therapeutic genes in the infected cells was downregulated by a caspase-8 inhibitor (Z-IETD-FMK) and upregulated by 5-FU (a activator of caspase-8), suggesting that cleavage of TRAIL-IETD-Smac fusion protein by activation of caspase-8. In a s.c. xenograft hepatocellular carcinoma (HCC) model, the antitumor effect of ZD55-TRAIL-IETD-Smac was significantly stronger than that of ZD55-TRAIL and ZD55-Smac, respectively. Importantly, intratumoral injection of ZD55-TRAIL-IETD-Smac eliminated all xenograft tumors established from a human HCC cell line Bel-7404, resulting in long-term, tumor-free survival. In conclusion, our data suggest that oncolytic adenovirus incorporating two different therapeutic genes by introduction of the IETD sequence may be attractive candidates for clinical application in treatment of cancer.

关键词： TRAIL Smac co-expression IETD virotherapy genetherapy

Multi-gene co-expression can improve comprehensive resistance to multiple abiotic stresses in Brassica napus L.

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Multi-gene co-expression can improve comprehensive resistanc...

中国作物学会油料作物专业委员会第八次会员代表大会暨学术...

作者： WANG Zai-qing YANG Cui-ling CHEN Hao WANG Pei WANG Peng-tao SONG Chun-peng ZHANG Xiao WANG Dao-jie Key Laboratory of Plant Stress Biology State Key Laboratory of Cotton BiologySchool of Life SciencesSchool of Mathematics and StatisticsHenan University

Rapeseed(Brassica napus L.) is an important oil crop worldwide.For current B.napus production,it is urgent to develop new varieties with higher seed productivity and increased stress tolerance for better adaptation to abiotic stresses as a result of global climate change.Genetic engineering,to some extent,can overcome limitations of genetic exchange in conventional breeding.consequendy,it considered as an effective method for improving modern crop breeding for B.napus.Since crop stress resistance is a complex polygenic trait,only multi-gene synergistic effects can effectively achieve the comprehensive stress resistance.Hence,in present study,5 stress resistance genes,NCED3,ABAR,CBF3,L0 S5 and ICE1 were transferred into B.napus.compared with wild type(WT) plants,multi-gene transformants K15 exhibited pronounced growth advantage under bodi normal growdi and stress conditions.Additionally,K15 plants also showed significantly higher resistance response to multiple stresses at seed germination and seedling stages than WT plants.Furthermore,K15 plants had significantly higher leaf temperature lower stomatal aperture and water loss rate than WT plants,which indicated that water-holding capacity of K15 plants was significantly superior to that of WT plants after stress treatment.In addition,K15 plants had significantly higher abscisic acid(ABA) content and remarkably lower malondialdehyde(MDA) content than WT plants.In conclusion,the above results suggested that multi-gene co-expression could rapidly trigger plant stress resistance,reduce the stress injury and synergistically improve comprehensive resistance of B.napus.

关键词： Brassica napus L. abiotic stress co-expression comprehensive stress resistance multi-genes transformation

construction and secretory co-expression of Amylase and Cellulase in Lactobacillus Casei

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Construction and secretory Co-expression of Amylase and Cell...

中国畜牧兽医学会动物微生态学分会第十一次全国学术研讨会...

作者： DING Ke YU Zu-hua Li Wang Li Yuan-xiao CHENG Xiang-chao ZHANG Chun-jie Jia Yan-yan He Lei Hongxiang Biological Feed Laboratory of Henan University of Science and Technology Animal Disease and Public Security Academician Workstation of Henan Province Military Veterinary Institute.Academy of Military Medical Sciences

This study is to construct a recombinant vector co-expressing amylase and cellulase,and examine the enzyme activities in Lactobacillus.The signal peptide gene SP,amylase and cellulase genes were cloned by PCR method,respectively,and inserted into plasmid pIlac according to special order to obtain the recombinant expressive vector pIlac-SP-AMY-CEL.Then this recombinant plasmid was transformed into Lactobacillus casei CECT5276 by electroporation methods.The gene sequences,enzyme activities,and hereditary stability were studied.The signal peptide gene,amylase gene and cellulase gene were 100%,98.9%and 99.6% similar to the GenBank corresponding sequences.SDS-PAGE indicated there was a special band at 114 kDa in both supemant and cell samples of recombinant Lactobacillus casei CECT5276/ pllac-SP- AMY-CEL.The total enzymes activity of amylase and cellulase were 35.65U/mL and 0.91U/mL in positive transformants,and its secretion rate was 70%.The recombinant Lactobacillus appeared more hereditary stability when they were blind cultured 50 generations under erythromycin selective pressure.The results revealed that the secretive fusion expression system of amylase and cellulase was constructed successfully,the amylase and cellulase could be produced in supernatant of the recombinant Lactobacilus,This recombinant Lactobacillus has a number promising properties that make it candidate for application as a probiotic additive in farm industry.

关键词： co-expression amylase cellulase Lactobacillus casei

Phenylalanine biosynthesis in Brevibacterium lactofermentum using Escherichia coli genes pheA , aroG and tyrB

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Progress in Natural Science:Materials International 2001年第10期 68-73页

作者：范长胜江培翃曾小冰吴永庆陈永青黄伟达 China Fudan University School of Life Sciences Shanghai 200433

Genetic engineering technology to increase the production of L-phenylalanine was used in the study. Three genes encoding the key enzymes involved in the biosynthesis of L-phenylalanine were utilized, in which the gene aroG encodes 3-deoxy-D-arabino-heptulosonate-7-phosphate synthetase (DS); the gene pheA encodes bifunctional enzyme of chorisate mutase (CM) and prephenate dehydratase (PD); and the gene tyrb encodes aminotransferase (AT). The three genes were amplified by polymerase chain reaction (PCR) from the genome of the E. coli mutant strains resistant to fluro-DL-phenylalanine and inserted into the cloning vectors. Then, they were expressed in E. coli and Bre-vibacterium lactofermentum in a tandem arrangement. The expressed enzymes had high activities in the host cells.

关键词： aroG pheA and tyrB genes co-expression L-phenylalanlne biosynthesis.