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Detection of Fish Disease Caused by Iridovirus on Grouper （Epinephelus sp.） and Pomfret Star （Trachinotus blochii） with co-agglutination Method in Tanjungpinang, Indonesia

引用

Journal of Agricultural Science and Technology(B) 2016年第2期6卷 121-128页

作者： Surya Amanu Dwi Sulistiyono Inyoman Suardana Division of Microbiology Faculty of Veterinary Medicine Gadjah Mada University Yogyakarta 560862 Indonesia Tanjungpinang Fish Quarantine and Inspection Agency Tanjungpinang 29113 Indonesia

Iridovirus infection often causes death and considerable economic losses in the aquaculture industry. This research applies the co-agglutination method that is fast, cheap and accurate in confirming the diagnosis of the cause of an outbreak of illness caused by iridovirus in the field, so that remedial action can be taken quickly and appropriately to minimize the impact of wider losses. Samples were taken from the grouper and pomfret star farms that are experiencing outbreaks of infectious diseases in the months from May to August 2015, Tanjungpinang, Indonesia. The sick and allegedly attacked by iridovirus samples showed abnormal swimming clinical symptoms, weakness and the swollen spleen. The swollen spleen of sick fish created suspension in phosphate buffered saline （PBS） with pH 7.2, and then centrifuged at 8,000 rpm for I0 rain. The supernatant after centrifuge was used as the test sample. On a clean object glass, 50 μL of the supematant was treated with 50 μL kit co-agglutination pre-prepared. The positive results were shown by the agglutination reaction.after 10-15 rain, while as a negative control, PBS was reacted with co-agglutination kit that looked homogeneous （no agglutination）. It was showed that the grouper （Epinepkelus sp.） on four farms and pomfret star （Thracinotus blochii） on one farm that experienced an outbreak of infectious disease in Tanjungpinang showed positively infected iridovirus. The same positive iridovirus result was also demonstrated by examination using polymerase chain reaction.（PCR） at 570 bp. So, the causative agent of plague on grouper and pomfret star was iridovirus. In addition, the co-agglutination test based on serology is more quick, cheap and accurate.

关键词： Iridovirus grouper pomfret star co-agglutination and polymerase chain reaction.

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