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cloning and sequence analysis of chitin synthase gene fragments of Demodex mites

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第10期13卷 763-768页

作者： Ya-e ZHAO Zheng-hang WANG Yang XU Ji-ru XU Wen-yan LIU Meng WEI Chu-ying WANG Department of Immunology and Pathogen Biology Xi'an Jiaotong University College of MedicineXi'an 710061China

To our knowledge,few reports on Demodex studied at the molecular level are available at *** this study our group,for the first time,cloned,sequenced and analyzed the chitin synthase(CHS) gene fragments of Demodex folliculorum,Demodex brevis,and Demodex canis(three isolates from each species) from Xi'an China,by designing specific primers based on the only partial sequence of the CHS gene of *** from Japan,retrieved from *** show that amplification was successful only in three *** isolates and one *** isolate out of the nine Demodex *** obtained fragments were sequenced to be 339 bp for *** and 338 bp for *** CHS gene sequence similarities between the three Xi'an *** isolates and one Japanese *** isolate ranged from 99.7% to 100.0%,and those between four *** isolates and one *** isolate were 99.1%-99.4%.Phylogenetic trees based on maximum parsimony(MP) and maximum likelihood(ML) methods shared the same clusters,according with the traditional *** open reading frames(ORFs) were identified in each CHS gene sequenced,and their corresponding amino acid sequences were located at the catalytic *** relatively conserved sequences could be deduced to be a CHS class A gene,which is associated with chitin synthesis in the integument of Demodex mites.

关键词： Demodex Chitin synthase genes cloning and sequencing Phylogenetic study Function analysis

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Isolation and sequence analysis of a Dof protein gene(PtDof1) in Populus

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Forestry Studies in China 2009年第2期11卷 93-98页

作者： XIN Bei CHEN Xiao-yang OUYANG Kun-xi PIAN Rui-qi LI Wei Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of Education Beijing Forestry University Beijing 100083 P. R. China College of Forestry South China Agricultural University Guangzhou 200064 P. R. China

Both cDNA and DNA clones of PtDof1 （GenBank Accession No. FJ402844 and FJ402845） were isolated from plants grown in tissue culture ofPopulus tornentosa. The DNA sequence is 1597 bp including two exons and one intron. The cDNA is 969 bp in length with a 765 bp open reading frame which is capable of encoding 255 amino acids. The deduced amino acids sequence of the PtDofl protein shares 65%, 56% and 55% identity with Vitis vinifera （CAO48618）, Nicotiana tabacum （CAA08755） and Glycine max （ABI 16022） Dof protein by blast analysis in GenBank. Phylogenic analysis suggests PtDof1 gene could belong to the Dofgene family. PtDofl protein contains an unusual conserved single zinc finger with the pattern of C-X2-C-X21-C-X2-C, which may play a functional role in tissue-specific expression and possibly the auxin response of endogenous plant genes.

关键词： Populus tomentosa Dof gene family PtDof1 cloning and sequencing

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