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Advances in Microbiology 2014年第12期4卷 754-760页

作者： Valérie Lalande Simon Barnabé Jean-Charles Coté Département de Biologie Médicale Université du Québec à Trois-RivièresTrois-RivièresCanada Agriculture and Agri-Food Canada Research and Development CentreSaint-Jean-sur-RichelieuCanada Département de Chimie Biochimie et PhysiqueUniversité du Québec à Trois-RivièresTrois-RivièresCanada

Biofouling, the accumulation of microorganisms, is a major problem in paper mills processing paper and cardboard. This leads to the production of lower quality recycled products. Several studies have focused on the microbial content in the paper mill and the final products. Our aim was to determine the microbial biota in a bale of collected cardboard prior to entering the paper mill. Total genomic DNA was isolated and analyzed using two different methods for comparison purposes: 454 pyrosequencing and clone library. A total of 3268 V6-V8 454 pyrosequencing reads and 322 cloned V6-V8 16S rRNA nucleotide sequences were obtained. Both methods showed the presence of three major bacterial genera: Bacillus, Solibacillus and Paenibacillus, all members of the spore-forming phylum Firmicutes. Pyrosequencing, however, revealed a richer and more diverse bacterial community than clone library. It showed the presence of additional minor Firmicute genera and of a small number of Proteobacteria. The sorting at the recycling plant, the storing, and the processing at the paper mill, the end uses, will all contribute to the bacterial microbiota present in a bale of collected cardboard as revealed here.

关键词： 454 Pyrosequencing Bacterial Microbiota Cardboard clone library Paper Mill

Characterization of the airborne bacteria community at different distances from the rotating brushes in a wastewater treatment plant by 16S rRNA gene clone libraries

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Journal of Environmental Sciences 2013年第1期25卷 5-15页

作者： Yunping Han Lin Li Junxin Liu Department of Water Pollution Control Technology Research Center for Eco-Environmental SciencesChinese Academy of Sciences

Biological risks of bioaerosols emitted from wastewater treatment processes have attracted wide attention in the recent years. However, the culture-based analysis method has been mostly adopted for detecting the bacterial community in bioaerosols, which may result in the underestimation of total microorganism concentration as not all microorganisms are cultivable. In this study, oligonucleotide fingerprinting of 16S rRNA genes was applied to reveal the composition and structure of the bacterial community in bioaerosols from an Orbal oxidation ditch in a Beijing wastewater treatment plant （WWTP）. Bioaerosols were collected at different distances from the aerosol source, rotating brushes, and the sampling height was 1.5 m which is the common respiratory height of a human being. The bacterial communities of bioaerosols were diverse, and the lowest bacterial diversity was found at the sampling site just after the rotating brush rotating brush. A large proportion of bacteria in bioaerosols were affiliated with Proteobacteria and Bacteroidetes. Numerous bacteria present in the bioaerosols also emerged in water, indicating that the bacterial community in the bioaerosols was related to that of the aerosols＇ sources. The forced aeration of rotating brushes brought about observably distinct bacterial communities between sampling sites situated before and after the rotating brush. Isolation sources of closest relatives in bioaerosols clone libraries were associated with the aqueous environment in the WWTP. Common potential pathogens in bioaerosols as well as those not reported in previous research were also analyzed in this study. Measures should be adopted to reduce the emission of bioaerosols and prevent their exposure to workers.

关键词： airborne bacteria genetic structure clone library wastewater treatment plant rotating brushes

Reduction and characterization of bioaerosols in a wastewater treatment station via ventilation

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Journal of Environmental Sciences 2014年第8期26卷 1575-1583页

作者： Xuesong Guo Pianpian Wu Wenjie Ding Weiyi Zhang Lin Li Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Regional Water Quality Control Board Los AngelesCA 90013USA Beijing University of Civil Engineering and Architecture

Bioaerosols from wastewater treatment processes are a significant subgroup of atmospheric aerosols. In the present study,airborne microorganisms generated from a wastewater treatment station（WWTS） that uses an oxidation ditch process were diminished by *** sampling and detection methods combined with cloning/sequencing techniques were applied to determine the groups,concentrations,size distributions,and species diversity of airborne microorganisms before and after ventilation. There were 3021 ± 537 CFU/m3 of airborne bacteria and 926 ± 132 CFU/m3 of airborne fungi present in the WWTS *** showed that the ventilation reduced airborne microorganisms significantly compared to the air in the WWTS. Over 60% of airborne bacteria and airborne fungi could be reduced after4 hr of air exchange. The highest removal（92.1% for airborne bacteria and 89.1% for fungi） was achieved for 0.65–1.1 μm sized particles. The bioaerosol particles over 4.7 μm were also reduced effectively. Large particles tended to be lost by gravitational settling and small particles were generally carried away,which led to the relatively easy reduction of bioaerosol particles0.65–1.1 μm and over 4.7 μm in size. An obvious variation occurred in the structure of the bacterial communities when ventilation was applied to control the airborne microorganisms in enclosed spaces.

关键词： Wastewater treatment station Vertical oxidation ditch Bioaerosols Genetic clone library

Abundance and diversity of ammonia-oxidizing archaea in response to various habitats in Pearl River Delta of China, a subtropical maritime zone

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Journal of Environmental Sciences 2013年第6期25卷 1195-1205页

作者： Zhixin Li Wenbiao Jin Zhaoyun Liang Yangyang Yue Junhong Lv Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control Harbin Institute of Technology Shenzhen Graduate School Shenzhen Institute of Advanced Technology Chinese Academy of Sciences

Ammonia-oxidizing archaea （AOA） are widely considered key to ammonia oxidation in various environments. However, little work has been conducted to simultaneously investigate the abundance and diversity of AOA as well as correlations between archaeal amoA genotypes and environmental parameters of different ecosystems at one district. To understand the abundance, diversity, and distribution of AOA in Pearl River Delta of China in response to various habitats, the archaeal amoA genes in soil, marine, river, lake, hot spring and wastewater treatment plant （WWTP） samples were investigated using real-time fluorescent quantitative PCR and clone libraries. Our analyses indicated that the diversity of AOA in various habitats was different and could be clustered into five major clades, i.e., estuary sediment, marine water/sediment, soil, hot spring and Cluster 1. Phylogenetic analyses revealed that the structure of AOA communities in similar ecological habitats exhibited strong relation. The canonical correspondence method indicated that the AOA community structure was strongly correlated to temperature, pH, total organic carbon, total nitrogen and dissolved oxygen variables. Assessing AOA amoA gene copy numbers, ranging from 6.84× 10^6 to 9.45 × 10^7 copies/g in dry soil/sediment, and 6.06× 10^6 to 2.41 ×10^7 copies/L in water samples, were higher than ammonia-oxidizing bacteria （AOB） by 1-2 orders of magnitude. However, AOA amoA copy numbers were much lower than AOB in WWTP activated sludge samples. Overall, these studies suggested that AOA may be a major contributor to ammonia oxidation in natural habitats but play a minor role in highly aerated activated sludge. The result also showed the ratio of AOA to AOB amoA gene abundance was positively correlated with temperature and less correlated with other environmental parameters. New data from our study provide increasing evidence for the relative abundance and diversity of ammonia-oxidizing archaea in the global nitrogen cyc

关键词： ammonia-oxidizing archaea abundance community structure real-time quantitative PCR clone library Pearl River Delta

Comparison of Bacterioplankton Communities in Three Heavily Polluted Streams in China

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Biomedical and Environmental Sciences 2011年第2期24卷 140-145页

作者： HUANG Yi ZOU Li ZHANG ShuYing XIE ShuGuang College of Environmental Sciences and Engineering Peking University Beijing 100871 China

Objective To compare the bacterioplankton communities in streams exposed to pollution of different types. Methods The bacterioplankton communities in three selected heavily polluted streams were investigated by using terminal‐restriction fragment length polymorphism （T‐RFLP） analysis in combination with 16S rRNA gene clone library analysis. Results Both T‐RFLP and 16S rRNA gene clone library revealed a great difference in bacterioplankton community composition in the different streams. Conclusion This work might provide some new insights into bioremediation of heavily polluted streams.

关键词： Bacterioplankton community Terminal‐restriction fragment length polymorphism (T‐RFLP) Streams clone library

Abundance and distribution of ammonia-oxidizing archaea in Tibetan and Yunnan plateau agricultural soils of China

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Frontiers of Environmental Science & Engineering 2014年第5期8卷 693-702页

作者： Kun DING Xianghua WEN Liang CHEN Daishi HUANG Fan FEI Yuyang LI Environmental Simulation and Pollution Control State Key Joint Laboratory School of Environment Tsinghua University Beijing 100084 China

As low oxygen and high ultraviolet （UV） exposure might significantly affect the microbial existence in plateau, it could lead to a specialized microbial community. To determine the abundance and distribution of ammonia-oxidizing archaea （AOA） in agricultural soil of plateau, seven soil samples were collected respectively from farmlands in Tibet and Yunnan cultivating the wheat, highland-barley, and colza, which are located at altitudes of 3200-3800 m above sea level. Quantitative PCR （q-PCR） and clone library targeting on amoA gene were used to quantify the abundances of AOA and ammonia-oxidizing bacteria （AOB）, and characterize the community structures of AOA in the samples. The number of AOA cells （9.34 × 10^7-2.32× 10^8 g^-1 soil） was 3.86-21.84 times greater than that of AOB cells （6.91 × 10^6-1.24 × 10^8 g^-1 soil） in most of the samples, except a soil sample cultivating highland- barley with an AOA/AOB ratio of 0.90. Based Kendall＇s correlation coefficient, no remarkable correlation between AOA abundance and the environmental factor was observed. Additionally, the diversities of AOA community were affected by total nitrogen and organic matter concentration in soils, suggesting that AOA was probably sensitive to several environmental factors, and could adjust its community structure to adapt to the environmental variation while maintaining its abundance.

关键词： ammonia-oxidizing archaea ammonia-oxidizing bacteria quantitative PCR clone library plateau

Phylogenetic diversity of ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit genes of bacterioplankton in the East China Sea

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Acta Oceanologica Sinica 2004年第4期23卷 673-685页

作者： ZENGYonghui JIAONianzhi CAIHaiyuan CHENXihan WEIChaoling KeyLaboratoryforMarineEnvironmentalScienceoftheMinistryofEducation EnvironmentalScienceResearchCenterXiamenUniversityXiamen361005China

Phylogenetic diversity of Form I and Form II ribulose1, 5-bisphosphate carboxylase/oxygenase (RubisCO) largesubunit (rbcL) genes in the inshore and offshore areas of the East China Sea were investigated. Two new primer setswere designed for amplifying partial sequences of rbcL genes from Proteobacteria. Four rbcL gene clone libraries wereconstructed by amplification and cloning of approximately 640~800 bp sequences of bacterioplankton *** method of screening library by denaturing gradient gel electrophoresis (DGGE) was introduced. The resultsshow that the diversity of Form I is higher in offshore waters with higher salinity and lower productivity, while thatof Form II is higher at the inshore station where salinity is lower and productivity is higher. Several clusters ofsequences obtained are deeply rooted and show low similarity (60%~78%) to the known rbcL in existing *** degree of diversity of rbcL genes is directly related to environmental variables, including temperature, salinity,pH, dissolved oxygen, etc. These results indicate that rbcL gene can be used as an effective indicator for geneticdiversity and population variability of bacterioplankton with the ability of carbon dioxide fixation in the sea.

关键词： RubisCO rbcL gene phylogenetic analysis clone library DGGE East China Sea

Molecular Analysis of Dominant Microbial Populations in Heavily and Slightly Polluted Aquifers by a Seaside Landfill

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Tsinghua Science and Technology 2005年第4期10卷 517-522页

作者：田扬捷杨虹李道棠吴秀娟 School of Life Science and Biotechnology Shanghai Jiao Tong University Shanghai 200240 China

The microbial populations were investigated in two groundwater samples, GW-H and GW-S, which represented heavily and slightly polluted aquifers by a seaside landfill. The concentrations of dissolved redox-relevant species suggested that iron-reduction/sulfate-reduction and denitrification were major redox processes for GW-H and GW-S. The dominant microbial populations were determined using restriction fragment length polymorphism analyses of 16S rRNA gene clone libraries. These microbes were then further studied by sequencing and phylogenetic analyses. The results indicate an obvious variation of the dominant populations between the two samples. The coexistence of sequences related to denitrifiers, sulfur-reducers, and methanotrophic bacteria was found in the GW-S sample, and a sequence associated with a sulfate-reducer was also found in the GW-H sample using molecular analyses. These results suggest that the molecular approach may be an important supplement to other approaches in characterizing the redox processes in polluted aquifers.

关键词： saline groundwater landfill 16S rRNA clone library microbial diversity

Recent investigation of indigenous microbial molecular research in YouShaShan oil field

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Recent investigation of indigenous microbial molecular resea...