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Molecular Epidemiology of Klebsiella pneumoniae from clinical Bovine mastitis in Northern Area of China,2018–2019

Engineering 2022年第3期8卷 146-154页

作者： Shikai Song Wenjuan He Dawei Yang Manar Benmouffok Yao Wang Jiyun Li Chengtao Sun Xiangbin Song Shizhen Ma Chang Cai Shuangyang Ding Congming Wu Zhangqi Shen Yang Wang Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China China Institute of Veterinary Drug Control Beijing 100081China College of Veterinary Medicine Hunan Agricultural UniversityChangsha 410125China Research and Innovation Office Murdoch UniversityMurdoch 6150Australia China Australia Joint Laboratory for Animal Health Big Data Analytics College of Animal Science and TechnologyZhejiang Agricultural and Forestry UniversityHangzhou 311300China

Klebsiella pneumoniae(***,KpⅠ)is a predominate inducement of bovine mastitis,which is associated with high mortality and milk yield ***,data is lacking on the molecular characteristics of bovine ***,limiting the risk assessment of its transmission through the food ***,we investigated the prevalence of *** in 6301 clinical mastitis(CM)milk samples from dairy cattle in northern area of *** total,183 *** isolates were recovered,with detection rates of 3.0% and 2.8% in 2018 and 2019,*** human clinical ***,all CM *** isolates belonged to one of three phylogroups:KpⅠ(n=143),*** ***(KpⅡ-B)(n=37),and Klebsiella variicola(KpⅢ)(n=3).We detected the extendedspectrum β-lactamase-encoding genes bla_(SHV-2a),blac_(CTX-M-14),and bla_(CTX-M-15),as well as clpC,lpfA,lacI,lacZ,lacY,and the fecABDEIR operon in the KpⅠ isolates,which may contribute to their pathogenicity and host adaptability in *** high prevalence of KpⅠ in dairy farms may be problematic,as it showed relatively higher rates of antibiotic resistance and virulence gene carriage than the KpⅡ-B and KpⅢ ***,we observed distinct differences in population structure between CM-and human infection-associated KpⅠ isolates,with the genes associated with invasive infection in humans rarely being observed in bovine isolates,indicating that few CM-associated *** isolates pose a threat to human ***,bovine KpⅡ-B isolates shared a high level of nucleotide sequence identity with isolates from human infections and frequently carried the nitrogen-fixation gene nif,suggesting an association between KpⅡ-B isolates from cattle and humans,and plant-derived bacteria.

关键词： clinical mastitis Klebsiella pneumonia Molecular characteristics Population structure Antimicrobial resistance

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Comparative Proteomic Analysis of Plasma from clinical Healthy Cows and Mastitic Cows

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Agricultural Sciences in China 2009年第10期8卷 1263-1269页

作者： YANGYong-xin ZHAO Xing-xu ZHANG Yong College of Veterinary Medicine Gansu Agricultural University Lanzhou 730070 P.R.China Institute of Animal Husbandry and Veterinary Medicine Anhui Academy of Agricultural Sciences Hefei 230031 P.R. China

The current research presents the protein changes in plasma from healthy dairy cows and clinical mastitic cows using two- dimensional gel electrophoresis （2-DE）. After staining with silver nitrate and Coomassie Blue, differential expression proteins were detected by PDQuest 7.4 software, and then subjected to ion trap mass spectrometer equipped with a Surveyor HPLC System, differential spots of protein were identified. Three protein spots that originated from preparation gels were identified to be two proteins. Overall, haptoglobin precursor was up-regulated in cows infected with clinical mastitis and could be a mastitis-associated diagnostic marker, whereas SCGB 2A1 （secretoglobin, family 2A, member 1） was down-regulated protein. Plasma protein expression patterns were changed when cows were infected with mammary gland inflammation; it suggests that analysis of differential expression protein might be useful to clarify the mechanisms involved in the pathophysiology, and find new diagnostic markers of mastitis and potential protein targets for treatment.

关键词： dairy cows clinical mastitis plasma proteome

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