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Identification of the 2-tridecanone cis-acting element in the promoter of cytochrome P450 CYP6B7 in Helicoverpa armigera

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Insect Science 2018年第6期25卷 959-968页

作者： Li Xu Dong-Zhi Li Yuan-Yuan Luo Jian-Ying Qin Li-Hong Qiu College of Science China Agricultural UniversityBeijingChina

The expression level of cytochrome P450 genes in insects can be induced by plant allelochemicals,which is important for insects to adapt to host plants.Cytochrome P450CYP6B 7has been reported to be involved in pyethroid insecticide resistance in Heli- coverpa armigera,and its transcription level was induced by some inducers.Currently,the regulatory mechanism of the induced expression of CYP6B7remains unknown,although it is very important for understanding the detoxification mechanism to allelochemicals in host plants.The objective of the present study was to investigate the eis-acting ele- ment in the promoter of CYP6B7 mediating the inducible up-regulation of CYP6B7in H.armigera by 2-tridecanone.The promoter region of CYP6B7was cloned by genome walking technique and analyzed by transient transfeetion assay.Progressive 5'deletion of the promoter region of CYP6B7revealed that the relative luciferase activity of construct -320/+232could be significantly induced by 2-trideeanone.Further stepwise deletion between -320 and -238 bp found that construct -292/+232 could also be significantly induced by 2-tridecanone,but the adjacent construct -256/+232could not,suggesting the essential role of the sequence between -292 and --257 bp for 2-tridecanone induction. Nucleotide mutations between -292 and -281 bp had no influence on the induction ef- fect by 2-tridecanone,but nucleotide mutations between -280 and -257 bp significantly decreased the induction effect.These results demonstrated that the cis-acting element for 2-trideeanone induction was between -280 and -257 bp in the promoter of CYP6B7.

关键词： cis-acting element cytochrome P450 CYP6B7 Helicoverpa armigera induction 2-tridecanone

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Functional analysis of cis-acting sequences regulating root-specific expression in transgenic tobacco

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Chinese Science Bulletin 2002年第17期47卷 1441-1445页

作者： Lan Nan Huixin Lin Yucheng Guan Fan Chen Institute of Developmental Biology Chinese Academy of Sciences Beijing China College of Life Sciences Hubei University Wuhan China

Two different length fragments, RSF1 and RSF2 which contained the cis-acting sequences of root-specific gene TobRB7, were isolated from tobacco genome. The abilities of these fragments to direct root-specific expression were studied by fusing them to the β-glucuronidase (GUS) report gene with different directions. After the recombined vectors were transformed into tobacco, the expression pattern was performed by histochemical staining and the quantitative analysis of GUS activity. The data suggested that the cis-acting element of TobRB7 gene direct GUS expression not only as root-specific but also as bidirectional. In our studies, the short fragment, RSF2, performed stronger activity than RSF1 with any direction. The stronger activity of GUS expression was determined by reverse inserting of RSF1 or RSF2 than positive inserting.

关键词： root tissue-specific expression bidirectional promoter cis-acting element transgenic plant.

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Binding of HMG Proteins to the 5'_Flanking Sequence of Human β-Globin Gene

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Science China Chemistry 1993年第1期36卷 81-88页

作者：钱若兰陈雅娣宋秋宝胡玉龙 Shanghai Institute of Cell Biology Academia Sinica Shanghai 200031 PRC

Our previous studies have identified that there are at least three regulatory regions (two negative regions and one positive region) in the 5’-flanking sequence of human β-globin gene (-610 to +1 bp). The binding of HMG proteins to both negative regulatory regions was examined by the gel mobility shift and DNase I protection assays.In gel mobility shift assay,we observed that HMG proteins 1 and 2 could bind to both negative regulatory regions (NCR1 and NCR2).Using the gel shift competition assay,we identified that the binding proteins between the two regions are different from each other.DNase I protection analysis shows that HMG proteins 1 and 2 only bind to one site (between-560 and-533 bp) in NCR1.However,two protected regions can be detected in NCR2, one between-272 and-252 bp relative to the cap site, the other between-306 and-329 bp.We also observed that HMG proteins 14 and 17 could not bind to both negative regions, so it seems that HMG proteins 1 and 2 may play an important role in the

关键词： cis-acting regulatory sequences HMG proteins β-globin gene.

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Regulatory role of DREB transcription factors in plant drought, salt and cold tolerance

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Chinese Science Bulletin 2000年第11期45卷 970-975页

作者： K.Yamaguch-Shinozaki K.Shinozaki Japan International Research Center for Agricultural Sciences Tsukuba 305JapanLaboratory of Plant Molecular BiologyInstitute of Physical and Chemical Research305Japan

rd29A gene of Arabidopsis encodes a LEA-like hydrophilic protein, its expression is induced by drought, high-salt and cold stress. In the promoter region of rd29A gene, there are 2 ORE cis-acting elements involved in responses to these environmental stresses. 5 cDNAs (DREB1A-C and DREB2A-B) encoding DREB transcription factors, which specifically bind to ORE element and control the expression of reporter gene under drought, high-salt and stress, have been isolated by One-Hybrid screening method and with ORE element of rd29A promoter. DREB transcription factors and ORE element function in signal transduction of drought, high-salt and cold stress. One DREB transcription factor can control the expression of several target functional genes involved in plant tolerance to drought, high-salt and cold stress. Thus, it may be an effective strategy to achieve ideal, multiple and fundamental effect for improving plant stress-resistance by DREB gene transfer.

关键词： promoter cis-acting element transcription factor signal transduction gene expression.

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Structure and regulatory function of plant transcription factors

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Chinese Science Bulletin 2001年第4期46卷 271-278页

作者： LIU Qiang, ZHANG Guiyou & CHEN ShouyiDepartment of Biological Science and Biotechnology, Tsinghua Univorsity, Beijing 100084, China Department of Biological Science and Biotechnology, State Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China Department of Biological Science and Biotechnology Tsinghua University Beijing China Department of Biological Science and Biotechnology State Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China Institute of Genetics Chinese Academy of Sciences Beijing China

The expression of inducible genes in plants is regulated by specific transcription factors at the transcrip-tional level. A typical transcription factor usually contains a DNA-binding domain, a transcription regulation domain, a dimerization site and a nuclear localization domain. These functional domains define the characteristic, localization and regulatory role of a transcription factor. Transcription factors recognize and bind to specific cis-acting elements or interact with other proteins, and then activate or repress the transcription of target genes by their functional domains. In recent years, elucidation on the structure and function of transcription factors has become an important subject in plant molecular biology.

关键词： transcription factor DNA-binding domain cis-acting element.

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Establishment and application of both FLP and Cre site-specific recombination systems at the same position in the genome

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Chinese Science Bulletin 2000年第5期45卷 456-460页

作者： Jilong Chen Jie Liu Yulian Zhang Zhiwei Chen Zhenhua Jin Chinese Acad Sci Inst Dev Biol Beijing 100080 Peoples R China

Both FRT-FRT and LoxP-LoxP sites that are the target sepuences of site-specific recombinases have been constructed in a vector, called C4LFY, using the recombinant DNA technigue. C4LFY also contains P elements, 2 exons and 1 intron of Drosophila yellow gene, yellow promoter and enhancers, and flanking DNA. Since C4LFY made use of two pairs of FRT and LoxP sites, this vector included two site-specific recombination systems. C4LFY was then integrated into Drosophila genome by P-element-mediated germ line transformation. in the presence of the FLP or Cre recombinase, either FLP/FRT or Cre/LoxP recombination reaction was successfully created at the same position in the genome. Using this system, the molecular basis of yellow gene expression and regulation during development have been investigated. Results indicate that the tissue-specific expression of yellow gene is directly regulated by transcriptional enhancers. in addition, the 5’ and 3’ genomic sequences flanking the yellow gene have been

关键词： site-specific recombination transgene gene expression and regulation cis-acting elements.

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Developmental stage-specific factors in the mouse haematopoietic tissues binding to the 5'-flanking as-acting elements of humanε-globin gene

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Chinese Science Bulletin 1995年第9期40卷 778-783页

作者：严志江陈雅娣钱若兰 Shanghai Institute of Cell Biology Chinese Academy of Sciences Shanghai 200031 China

The human ε-globin gene is expressed in a tissue-specific and developmental stage-specific manner. During the earliest stage of gestation, this gene is expressed in the yolksac, but is silenced completely at the 6th-8th weeks of gestation. Recently, several studieson the transgenic mice have shown that the 5′-flanking DNA sequences of human

关键词： human ε-globin gene cis-acting element trans-acting factor.

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The single-stranded DNA binding protein WHIRLY1 represses WRKY53 expression and delays leaf senescence in a developmental stage-dependent manner in Arabidopsis thaliana

The single-stranded DNA binding protein WHIRLY1 represses WR...

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2013全国植物生物学大会

作者： REN Yujun JIANG Jingjing ZHAO Ziwei HUANG Dongmei MIAO Ying Center for Molecular Cell and Systems Biology College of Life SciencesFujian Agriculture and Forestry University Center of Plant Molecular Biology Department of General GeneticsUniversity of Tuebingen Institute of Botany Christian-Albrechts-University of Kiel

Leaf senescence in plants involves both positive and negative transcriptional regulations.Here we show evidence for the single-stranded DNA(ssDNA) binding protein WHIRLY1(WHY1) that functions as an up

关键词： WHIRLY1 WRKY53 promoter cis-acting element leaf senescence

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