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Tropomyosin is localized in the nuclear matrix and chromosome scaffold of physarum polycephalum

Cell Research 1999年第1期9卷 61-69页

作者： ZENG XIAN LU MING DA JIAO MIAO XING XIAO GUANG WANG SHUI HAO(Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China)(Department of Biology, Changchun Normal College,Changchun 130032, China) Institute of Genetics and Cytology Northeast Normal University Changchun China Department of Biology Changchun Normal College Changchun China

The nuclei and chromosomes were isolated from plasmodia of Physarum polycephalum. The nuclear matrir and chromosome scaffold were obtained after the DNA and most of the proteins were extracted with DNase I and 2 M NaCl. SDS-PAGE analyses revealed that the nuclear matrir and chromosome scaffold contained a 37 kD polypeptide which is equivalent to tropomyosin in molecular weight. Immunofluorescence observations upon slide preparations labeled with anti-tropomyosin antibody showed that the nuclear matrix and chromosome scaffold emanated bright fluorescence, suggesting the presence of the antigen in *** results confirmed the presence of tropomyosin in the nuclear matrix and chromosome scaffold. Immunoelectron microscopic obserwtions further demonstrated that tropomyosin was dispersively distributed in the interphase nuclei and metaphase chromosomes.

关键词： chromosome scaffold nuclear matrix Physarum polycephalum tropomyosin

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VISUALIZATION OF THE chromosome scaffold IN A PRIMITIVE EUKARYOTE DINOFLAGELLATE Crypthecoainium cohnii

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Science China Chemistry 1992年第8期35卷 959-965,1025-1026页

作者：蔡树涛翟中和曾丛梅李靖炎 Department of Biology Peking UniversityBeijing 100871PRC Kunming Institute of Zoology Academia SinicaKunming 650107PRC

The chromosome scaffolds in higher eukaryotic nuclei have been described elsewhere. Butit is unknown when they evolved. The dinoflagellates are the primitive organisms that maybe the intermediate between prokaryotes and eukaryotes. Combining chromosome scaffold prep-aration methods with embedment-free section microscopy,we demonstrate that the dino-flagellate Crypthecodinium cohnii chromosome retains a protein scaffold after the depletionof DNA and soluble proteins. This scaffold preserves the morphology characteristic of thechromosome. Two-dimensional electrophoreses show that the chromosome scaffolds are mainlycomposed of acidic proteins. Our results suggest that a framework similar to the chromosomescaffold in the mammalian cell appeared in the primitive eukaryote. We propose that thechromosome scaffold possibly originated from the early stages of eukaryote evolution.

关键词： chromosome scaffold chromosome dinoflagellate Crypthecodinium cohnii

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Identification of the nuclear matrix and chromosome scaffold in dinoflagellate Crythecodinium cohnii

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Cell Research 1992年第2期2卷 165-181页

作者： CAT SHUTAO , CONGMEI ZENG, JINGYAN LI AND ZHONGHE ZHAI Department of Biology, Beijing University, Beijing 100871, China. Kunming Institute of Zoology, Academia Sinica, Kunming 650107, China. Department of Biology Beijing University Beijing China Kunming Institute of Zoology Academia Sinica Kunming China

Dinoflagellate is one of the primitive eukaryotes,whosenucleus may represent one of the transition stages fromprokaryotic nucleoid to typical eukaryotic *** extraction together with embeddment-free sectionand whole mount electron microscopy,a delicate nuclearmatrix filament network was shown,for the first time,indinoflagellate Crypthecodinium cohnii *** are connected with nuclear matrix filaments to forma complete network spreading over the ***,we demonstrated that the dinoflagellate chromosome retainsa protein scaffold after the depletion of DNA and *** scaffold preserves the characteristic mor-phology of the *** dimensional elec-trophoreses indicated that the nuclear matrix and chromo- some scaffold are mainly composed of acidic *** demonstrated that a framework similar to the nuclearmatrix and chromosome scaffold in mammalian cells appearsin this primitive eukaryote,suggesting that these structuresmay have been originated from the early stages of eukaryoteevolution.

关键词： nuclear matrix chromosome scaffold dinoflagellate Crypthecodinium cohnii.

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