检索结果-南通市图书馆

AtCLH2, a Typical but Possibly Distinctive chlorophyllase Gene in Arabidopsis

Journal of Integrative Plant Biology 2007年第4期49卷 531-539页

作者： Yang Liao Kun An Xiao Zhou Wen-Jun Chen Ben-Ke Kuai State Key Laboratory of Genetic Engineering Department of Biochemistry School of Life Sciences Fudan University Shanghai 200433 China

chlorophyllase （EC 3.***.1.14） is involved in the first step of chlorophyll degradation. Isolation of chlorophyllase genes greatly facilitates characterization of chlorophyllase properties and elucidation of molecular regulation of their in vivo activities. There are two chlorophyllase genes, AtCLH1 and AtCLH2, in Arabidopsis thaliana. The in vivo roles of AtCLH1 have been reported previously. However, few studies have been carried out on AtCLH2. Here, we show that purified recombinant Chlase2, encoded by AtCLH2, exhibits in vitro chlorophyllase activity. Interestingly, ＂activation＂ of in vitro activity of the recombinant Chlase2 required higher concentrations of a detergent or a polar solvent. To determine its activity in vivo, the expression of AtCLH2 was inhibited by RNA interference. RNAi plants showed decreased contents of chlorophyllide without a substantial change in the total amount of the extractable chlorophyll and consequently presented lower chlorophyllide to chlorophyll ratios in their leaves. In addition, the two AtCLHs exhibited differential expression patterns. Our results suggest that AtCLH2 might play a distinctive role in chlorophyll catabolism in vivo.

关键词： Arabidopsis AtCLH1 AtCLH2 chlorophyllase chlorophyll degradation RNA interference.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Arahidopsis chlorophyllase 1 protects young leaves from long-term photodamage by facilitating FtsH-mediated D1 degradation in photosystemⅡrepair

引用

Molecular Plant 2021年第7期14卷 1149-1167页

作者： Ya-nan Tian Rui-hao Zhong Jun-bin Wei Hong-hui Luo Yoram Eyal Hong-lei Jin La-jie Wu Ke-ying Liang Ying-man Li Shu-zhen Chen Zhao-qi Zhang Xue-qun Pang State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables South China Agricultural UniversityGuangzhou 510642People's Republic of China College of Life Sciences South China Agricultural UniversityGuangzhou 510642People's Republic of China College of Horticulture South China Agricultural UniversityGuangzhou 510642People's Republic of China lnstitute of Plant Sciences The Volcani CenterAgricultural Research OrganizationBet Dagan 50250Israel lnstituteof Medical Plant Physiology and Ecology School of Pharmaceutical SciencesGuangzhou University of Chinese MedicineGuangzhou 510006People's Republic of China

The proteolytic degradation of the photodamaged D1 core subunit during the photosystemⅡ(PSⅡ)repair cycle is well understood,but chlorophyll turnover during D1 degradation remains ***,we report that Arabidopsis thaliana chlorophyllase 1(CLH1)plays important roles in the PSII repair *** abundance of CLH1 and CLH2 peaks in young leaves and is induced by high-light *** of clh1 single and clh1-1/2-2 double mutants display increased photoinhibition after long-term high-light exposure,whereas seedlings overexpressing CLH1 have enhanced light tolerance compared with the wild ***1 is localized in the developing chloroplasts of young leaves and associates with the PSⅡ-dismantling complexes RCC1 and RC47,with a preference for the latter upon exposure to high ***,degradation of damaged D1 protein is retarded in young clh1-1/2-2 leaves after 18-h highlight exposure but is rescued by the addition of recombinant CLH1 in ***,overexpression of CLH1 in a variegated mutant(var2~2)that lacks thylakoid protease FtsH2,with which CLH1 interacts,suppresses the variegation and restores D1 degradation.A var2-2 clh1-1/2-2triple mutant shows more severe variegation and seedling *** together,these results establish CLH1 as a long-sought chlorophyll dephytylation enzyme that is involved in PSⅡrepair and functions in long-term adaptation of young leaves to high-light exposure by facilitating FtsH-mediated D1 degradation.

关键词： chlorophyllase photosystemⅡrepair young leaf D1 degradation photoprotection chlorophyll dephytylation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：