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Transcriptome analysis to identify candidate genes related to chlorogenic acid biosynthesis during development of Korla fragrant pear in Xinjiang

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Food Science and Human Wellness 2022年第4期11卷 854-864页

作者： Hao Wen Wenqiang Wang Xi Jiang Minyu Wu Hongjin Bai Cuiyun Wu Lirong Shen Department of Food Science and Nutrition Zhejiang Key Laboratory for Agro-Food ProcessingZhejiang UniversityHangzhou 310058China College of Horticulture Forestry Tarim University of Land-ReclamationAral 843300China

Korla fragrant pear(KFP)with special fragrance is a unique cultivar in Xinjiang,*** order to explore the biosynthesis molecular mechanism of chlorogenic acid(CGA)in KFP,the samples at different development periods were collected for transcriptome *** performance liquid chromatography analysis showed that CGA contents of KFP at 88,118 and 163 days after full bloom were(20.96±1.84),(12.01±0.91)and(7.16±0.41)mg/100 g,respectively,and decreased with the fruit *** from these typical 3 periods were selected for de novo transcriptome assemble and 68059 unigenes were assembled from 444037960 clean ***‘phenylpropanoid biosynthesis’pathway including 57 unigenes,11 PALs,1 PTAL,64CLs,9 C4Hs,25 HCTs and 5 C3’Hs related to CGA biosynthesis was *** was found that the expression levels of 11 differentially expressed genes including 1 PAL,2 C4Hs,34CLs and 5 HCTs were consistent with the change of CGA *** polymerase chain reaction analysis further showed that 8 unigenes involved in CGA biosynthesis were consistent with the RNA-seq *** findings will provide a comprehensive understanding and valuable information on the genetic engineering and molecular breeding in KFP.

关键词： Korla fragrant pear Development period chlorogenic acid content De novo transcriptome assembly Phenylpropanoid biosynthesis pathway

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Effects of different drying methods on drying kinetics and physicochemical properties of Chrysanthemum morifolium Ramat

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International Journal of Agricultural and Biological Engineering 2019年第3期12卷 187-193页

作者： Borui Li Jueyi Lin Zhian Zheng Hao Duan Dong Li Min Wu College of Engineering China Agricultural UniversityBeijing 100083China

The effects of infrared-assisted hot-air drying(IR-HAD),temperature,humidity controlled hot-air drying(THC-HAD),and hot-air drying(HAD)on the drying kinetics,physicochemical properties,chlorogenic acid content and microstructure of chrysanthemum were experimentally *** results showed that the drying time reduced with increasing air drying temperature,with IR-HAD needing the shortest drying time,followed by THC-HAD and *** effective moisture diffusivities(Deff)of chrysanthemum under IR-HAD,THC-HAD,and HAD at 60℃ were 3.22×10^(-9) m^(2)/s,2.19×10^(-9) m^(2)/s,and 2.89×10^(-9) m^(2)/s,***-HAD preserved chrysanthemum surface color better than THC-HAD and HAD,whereas the THC-HAD samples obtained higher water holding capacity(WHC),water binding capacity(WBC),and chlorogenic acid ***,peroxidase(POD)residual activity of the samples decreased with increasing blanching *** current work provides a theoretical basis for the drying of chrysanthemum.

关键词： chrysanthemum drying kinetics Weibull model physicochemical properties peroxidase(POD)residual activity chlorogenic acid content

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