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Tumor radioimmunoimaging of chimeric antibody in nude mice with hepatoma xenograft

World Journal of Gastroenterology 1998年第1期4卷 12-14页

作者： GONG Yi LIU Kang-Da ZHOU Ge XUE Qiong CHEN Shao-Liang TANG Zhao-You Liver Cancer Institute Zhongshan Hospital Shanghai Medical University Shanghai 200032 China Shanghai Medical University China Institute of Nuclear Medicine Zhongshan Hospital Shanghai Medical University Shanghai 200032 China

IM To study the radioimmunoimaging (RAII) using the human/mouse chimeric Ab to evaluate its targeting activity in animal models.METHODS To chimeric Ab was labeled with 131I. RAII was performed at different intervals after injection of radiolabeled Abs in nude mice with human hepatoma xenograft, and tissue distribution of radioactivity was measured. Comparison was made in the chimeric Ab between the single segment Ab and previous murine mAb against HBxAg.RESULTS The experimental objects developed tumorpositive image after 2 days of radiolabeled Abs injection, and the peak accumulation of radioactivity fell on the 7th day. The tumor/liver ratioactivity of the chimeric Ab, single segment Ab, antiHBx mAb, and the control group was 281±021, 244±016, 460±019, and 096±014, respectively.CONCLUSION The genetic engineering Abs have a considerable targeting activity which can be used as a novel humanized vector in the targeting treatment of liver cancer..

关键词： liver neoplasms, experimental carcinoma, hepatocellular chimeric antibody mice, nude hepatitis B virus disease models, animal radioimmunodetection radioimmunotherapy

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CONSTRUCTION AND EXPRESSION OF A HUMAN-MOUSE chimeric antibody AGAINST HUMAN BLADDER CANCER

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Chinese Journal of Cancer Research 2001年第4期13卷 271-275页

作者：白银王琰周丽君俞莉章 Central Laboratory Naval General Hospital，Beijing 100037Institute of Surgical UrologyThe First Hospital Peking University Beijing 100034 Central Laboratory Naval General Hospital，Beijing 100037 Institute of Surgical Urology The First Hospital Peking University Beijing 100034

Objective: To construct and express a human-mouse chimeric antibody against human bladder cancer. Method: The variable region genes of anti-human bladder cancer monoclonal antibody BDI-1 were cloned by RT-PCR. A human-mouse chimeric antibody expression vector was constructed and transfected into CHO cells. The chimeric antibody against bladder cancer was expressed and characterized. Result: Eukaryotic expression vector of the chimeric antibody against human bladder carcinoma was successfully constructed, and was expressed in eukaryotic cells; the expressed chimeric antibody ch-BDI showed same specificity as its parent McAb against human bladder cancer cells. Conclusion: The constructed chimeric antibody was expressed successfully in eukaryotic cells, and the chimeric antibody had desired affinity against human bladder cancer cells.

关键词： chimeric antibody Bladder cancer Eukaryotic expression

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Anti-Proliferative Effects Induced by Anti-CD4 Human/Murine chimeric antibody and Murine Anti-CD4 Monoclonal antibody

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Journal of Huazhong University of Science and Technology(Medical Sciences) 1999年第1期19卷 7-10页

作者：沈关心朱慧芬王晓林张悦朱志刚王硕 Department of Immunology Tongji Medical University Wuhan 430030

Summary: The effects of chimeric anti CD4 human/murine chimeric antibody and murine anti CD4 monoclonal antibody (McAb) on the proliferation induced by anti CD3 McAb, phytohemagglutinin (PHA), IL 2, and allogeneic cells were studied. The results showed that chimeric anti CD4 antibody and murine anti CD4 McAb could inhibit the proliferation induced by the above inducers and the inhibitory effects were related to the dosage of the antibodies.

关键词： CD4 molecule chimeric antibody monoclonal antibody inhibition of proliferation

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Preparation of Human-Mouse chimeric antibody against CD80 Molecule and Analysis of Its Inhibitory Effects on Human B Lymphoma Cell lines Raji and Daudi

Preparation of Human-Mouse Chimeric Antibody against CD80 Mo...

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中国病理生理学会受体、肿瘤和免疫专业委员会联合学术会议

作者： Yan-ru Wang,Yao-yu Xu,Yong-jing Chen,Hua-ting Zhu,Yu-hua Liu,Jie Sun,Yu-hua Qiu~* Department of Immunology,School of Medicine,Soochow University

AIM:To Prepare human-mouse chimeric antibody against CD80 molecule（named ch-4E5） and analyse inhibitory effects of ch-4E5 on the growth of B lymphoma cell lines Daudi and Raji. METHODS:pIRES/ch4E5 with chimeric heavy and light gene was *** expression of ch4E5 antibody in 293T cells was detected by flow cytometry（FCM）.CHO cells were transfected by pIRES/ch4E5 to construct stable cell line secreting chimeric *** ch4E5 antibody was purified from culture supernatant without fetal calf serum（FCS） by Protein G affinity chromatography,and then recognization of ch4E5 antibody to membrane CD80 molecules on Raji and Daudi cells were detected by immunofluorescence and *** the co-culture of ch-4E5 with Raji and Daudi,respectively,the expression of Fas and FasL was observated to be at the 0h,4h,10h,16h,24h and 48h by direct immunofluorescence *** inhibitory effect of ch-4E5 on Raji and Daudi celsl growth was determined at 72 h by MTT *** ADCC effect of ch-4E5 with PBMC as effector cells and Raji and Daudi as target cells was determined at 72 *** efficiency target ratio was 20:*** model was established using 4-6 weeks female BALB/c nude *** were parted to four groups randomly with 10 every *** 1:inject Raji cells only;group 2:Raji+Hu-IgG1;group 3:Raji+ch-4E5 antibody;group 4: Raji+4E5 antibody（mouse antibody）.Raji cells were inoculated into BABL/c nude mouse,at 5×10～6 per *** then on,we observed closely and recorded survival condition of nude mouse and calculate the gross tumor ***:Transient expression of ch4E5 antibody,whose combination rate with cell membrane CD80 molecules on Raji and Daudi was 96.0%by FCM,peaked expression at 48 hours in 293T *** ch4E5 antibody was stably expressed in CHO cells,whose combination rate was 95.7%.About 5-7 milligram ch4E5 antibodies were purified from each one liter cculture supernatant without *** combination rate between ch-4E5 and Raji and Daudi was

关键词： CD80 chimeric antibody inhibitory effect Fas FasL ADCC

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