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Curcumin cytotoxicity is enhanced by PTEN disruption in colorectal cancer cells

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World Journal of Gastroenterology 2013年第40期19卷 6814-6824页

作者： Lin Chen Wen-Feng Li Hong-Xiao Wang Hai-Na Zhao Jia-Jia Tang Chang-Jie Wu Li-Ting Lu Wan-Qin Liao Xin-Cheng Lu Institute of Genomic Medicine Wenzhou Medical University Department of Oncology First Affiliated Hospital of Wenzhou Medical University

AIM:To investigate the effects of phosphatase and tensin homolog deleted on chromosome 10(PTEN) deficiency on the cytotoxicity of chemotherapeutic agents toward colorectal cancer ***:PTEN-deficient colorectal cancer(CRC) cells were generated by human somatic cell gene targeting using the adeno-associated virus system. The cytotoxic effects of compounds including curcumin,5-fluorouracil(5-FU),dihydroartemisinin(DHA),irinotecan(CPT-11)and oxaliplatin(OXA) on cancer cells were determined using the MTT assay. Enhanced cytotoxicity of curcumin in PTEN-deficient CRC cells was observed,and this was confirmed using clonogenic assays. Apoptosis and cell cycle progression were analyzed by flow *** of apoptosis and cell cycle-related proteins were examined by Western ***:We developed an isogenic set of CRC cell lines that differed only in their PTEN status. Using this set of cell lines,we found that disruption of the PTEN gene had no effect on the sensitivity of CRC cells to5-FU,CPT-11,DHA,or OXA,whereas PTEN disruption increased the sensitivity of CRC cells to curcumin. Loss of PTEN did not alter the curcumin-induced apoptosis in CRC cells. However,PTEN deficiency led to an altered pattern of curcumin-mediated cell cycle *** HCT116 PTEN+/+cells,curcumin caused a G2/M phase arrest,whereas it caused a G0/G1 phase arrest in HCT116 PTEN-/-cells. Levels of cell cycle-related proteins were consistent with these respective patterns of cell cycle ***:Curcumin shows enhanced cytotoxicity toward PTEN-deficient cancer cells,suggesting that it might be a potential chemotherapeutic agent for cancers harboring PTEN mutations.

关键词： Phosphatase and tensin homolog deleted on chromosome 10 Curcumin chemotherapeutic agents Cell cycle AKT signaling

Long interspersed nuclear element ORF-1 protein promotes proliferation and resistance to chemotherapy in hepatocellular carcinoma

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World Journal of Gastroenterology 2013年第7期19卷 1068-1078页

作者： Fan Feng Yin-Ying Lu Fan Zhang Xu-Dong Gao Chuan-Fu Zhang Alex Meredith Zhong-Xian Xu Yu-Tao Yang Xiu-Juan Chang Hong Wang Jian-Hui Qu Zhen Zeng Jun-Lan Yang Chun-Ping Wang Yun-Feng Zhu Jia-Jun Cui Yong-Ping Yang Clinical Research Center of Hepatocarcinoma 302 Military Hospital Institute of Toxicology and Pharmacology Chinese Academy of Military Medical Sciences Tumor Center PLA General Hospital Institute of Disease Control and Prevention Chinese Academy of Military Medical Sciences Department of Cancer and Cell Biology University of Cincinnati College of MedicineCincinnatiOH 45267United States Beijing Institute for Neuroscience Capital Medical University

AIM:To clarify the specific roles and mechanisms of long interspersed nuclear element-1 ORF-1 protein [human long interspersed nuclear element-1(LINE-1),ORF-1p] in chemotherapeutic drug resistance and cell proliferation regulation in hepatocellular carcinoma(HCC) ***:MTT assays were performed to identify the effect of the chemotherapeutic drug toxicity on HepG2 *** proliferation inhibition and the IC 50 were calculated by the Origin 8.0 *** blotting assays were performed to investigate whether LINE-1 ORF-1p modulates the expression of some important genes,including p53,p27,p15,Bcl-2,mdr,and *** corroborate the proliferation and anchor-independent growth results,the HepG2 cells were analyzed by flow cytometry to investigate the effect of LINE-1 ORF1p on the apoptosis ***:LINE-1 ORF-1p contributed to the resistance to several chemotherapeutic drugs(cisplatin and epirubicin) in HepG2 *** IC 50 of the epirubicin and cisplatin increased from 36.04 nmol/L to 59.11 nmol/L or from 37.94 nmol/L to 119.32 nmol/*** of LINE-1 ORF-1p expression by the siRNA could markedly enhance the response of HepG2 cells to the epirubicin and *** IC 50 correspondingly decreased from 28.06 nmol/L to 3.83 nmol/L or from 32.04 nmol/L to 2.89 nmol/***,down-regulation of LINE-1 ORF-1p level by siRNA could promote the response of HepG2 cells to the *** IC 50 decreased from 35.90 nmol/L to 7.36 nmol/***,overexpression of LINE-1 ORF-1p did not modulate the paclitaxel toxicity in HepG2 *** Western blotting revealed that LINE-1 ORF-1p enhanced mdr and p-gp gene *** a protein arrested in the nucleus,LINE-1 ORF-1p may function through modulating transcriptional activity of some important transcription ***,LINE-1 ORF-1p promoted HepG2 cell proliferation,anchor-independent growth and protected the cells against apoptosis through modulating the expression of p15,p21,p53,and B

关键词： Long interspersed nuclear element-1 ORF-1 protein Hepatocellular carcinoma chemotherapeutic drugs Multi-drug resistance

Isolation and identification of adipose-derived stromal/stem cells from breast cancer patients after exposure neoadjuvant chemotherapy

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World Journal of Experimental Medicine 2020年第3期10卷 26-40页

作者： Ashleigh Rapp Hagaman Ping Zhang Kiavash R Koko Ryan S Nolan Marc W Fromer John Gaughan Martha Matthews Department of Surgery Cooper University HospitalCamdenNJ 08103United States Cooper Medical School of Rowan University CamdenNJ 08103United States

BACKGROUND With recent research advances,adipose-derived stromal/stem cells(ASCs)have been demonstrated to facilitate the survival of fat grafts and thus are increasingly used for reconstructive procedures following surgery for breast ***,in patients,following radiation and chemotherapy for breast cancer suggest that these cancer treatment therapies may limit stem cell cellular functions important for soft tissue wound *** clinical translation to patients that have undergone cancer treatment,it is necessary to understand the effects of these therapies on the ASC's ability to improve fat graft survival in clinical *** To investigate whether the impact on ASCs function capacity and recovery in cancer patients may be due to the *** ASCs were isolated from the cancerous side and noncancerous side of the breast from the same patients with receiving neoadjuvant chemotherapy(NAC)or notreceiving *** were in vitro treated with 5-fluorouracil(5-FU),doxorubicin(DXR),and cyclophosphamide(Cytoxan)at various *** stem cells yield,cell viability,and proliferation rates were measured by growth curves and MTT *** capacity for adipogenesis was determined by qPCR analysis of the specific gene markers and histological *** No significant differences were observed between the yield of ASCs in patients receiving NAC treatment and not-receiving *** yield from the cancerous side of the breast showed lower than the noncancerous side of the breast in both patients receiving NAC and not-receiving *** proliferation rates of ASCs from patients didn’t differ much before and after NAC upon in vitro culture,and these cells appeared to retain the capacity to acquire adipocyte traits simile to the ASCs from patients not-receiving *** cessation and washout of the drugs for another a week of culturing,ASCs showed a slow recovery of cell growth capacity in 5-FU-treated groups but was not

关键词： Human adipose-derived stromal/stem cells Breast cancer Neoadjuvant chemotherapy chemotherapeutic agents Adipogenic differentiation Proliferation

喜辽妥联合硫酸镁湿敷在血液肿瘤患儿化疗性静脉炎中的应用效果探究

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中国合理用药探索 2019年第12期16卷 138-140页

作者：曹文娟贾雯周一鸣张银花李彩霞郑州大学附属儿童医院/郑州儿童医院血液肿瘤科河南郑州450000

目的:探讨喜辽妥联合硫酸镁湿敷治疗血液肿瘤患儿化疗性静脉炎的临床效果。方法:选择2015年1—2016年10月我院儿科收治的90例因血液肿瘤化疗而放置留置针的患儿。按随机数字表法分为观察组和对照组,各45例。在放置留置针的过程中,对照... 详细信息

目的:探讨喜辽妥联合硫酸镁湿敷治疗血液肿瘤患儿化疗性静脉炎的临床效果。方法:选择2015年1—2016年10月我院儿科收治的90例因血液肿瘤化疗而放置留置针的患儿。按随机数字表法分为观察组和对照组,各45例。在放置留置针的过程中,对照组予硫酸镁湿敷,观察组联合应用硫酸镁湿敷和喜辽妥软膏。比较两组静脉炎发生率,发生静脉炎患儿的治愈时间,以及患儿在治疗过程中的舒适度。结果:观察组静脉炎发生率低于对照组,差异有统计学意义(P<0.05);在发生静脉炎后,观察组缓解时间短于对照组,差异有统计学意义(P<0.05);对于整个治疗过程中的舒适程度评价,观察组评分高于对照组,差异有统计学意义(P<0.05)。结论:喜辽妥联合硫酸镁湿敷能够有效预防化疗性静脉炎的发生,提高患儿在放置留置针期间的舒适度,并能在较短时间内治愈静脉炎,具有较高的临床应用价值。

关键词： Hirudoid Magnesium Sulfate Children with Hematological Tumors chemotherapeutic Phlebitis

Side Effects of Chemotherapy in Cancer Patients and Evaluation of Patients Opinion about Starvation Based Differential Chemotherapy

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Journal of Cancer Therapy 2014年第8期5卷 817-822页

作者： Muhammad Shahbaz Aslam Sidra Naveed Aftab Ahmed Zaigham Abbas Iram Gull Muhammad Amin Athar Department of Microbiology and Molecular Genetics University of the Punjab Lahore Pakistan Institute of Biochemistry & Biotechnology University of the Punjab Lahore Pakistan

Side-effects associated with the cancer chemotherapy limit the scope of chemotherapeutic drugs and no data was available about these side effects in Pakistan. Moreover starvation based differential chemotherapy has been proved to greatly reduce the side effects of chemotherapy depending on starvation time. The current study was conducted to survey the common side effects of the chemotherapeutic drugs and the role of starvation to reduce them. The study included total 100 subjects with multiple carcinomas. A comprehensive questionnaire about starvation inquiry, chemotherapy side effects and their basic information was filled by interviewers as told by patients. There were 48% patients with breast cancer and 11% with uterine cancer. Out of these patients 30%, 28%, 9% and 9% patients were agreed to starve for 12, 24, 36 and 48 hours respectively. The survey regarding the side effects of chemotherapy showed that 43% patients were suffering from headache, fatigue 90%, weakness 95%, hair loss 76%, nausea 77%, vomiting 75%, diarrhea 31%, abdominal cramps 40%, mouth sores 47%, dry mouth 74%, memory impairment 14%?and numbness 49%. Breast cancer is the most common cancer in Pakistan. Only 18% of the total patients were agreed to starve for more than one day. Chemotherapy-associated side effects vary greatly and it does not depend upon cancer type. But these side effects depend on multiple factors such as the type and dose of chemotherapeutic drug, patient’s health status and stage of cancer.

关键词： Starvation Differential Chemotherapy Side Effects chemotherapeutic Drugs

Fermented Ethnic Medicine (<i>Pueraria mirifica</i>) Designed by Lactobacillus Regulated Leukocyte Subsets via Activation of Complement Components

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Open Journal of Rheumatology and Autoimmune Diseases 2017年第1期7卷 65-79页

作者： Yoshiichiroh Mastuba Kazuhiro Okamoto Yuma Kato Nobuo Yamaguchi Sumiyo Akazawa-Kudoh Ishikawa Natural Medicinal Products Research Center Ishikawa Japan Department of Rehabilitation Kanazawa Medical University Uchinada-machi Japan Department of General Medicine Kanazawa Medical University Ishikawa Japan

A plant fermentation was carried out by Lactobaccilli against the Rhizome from Pueraria milifica (f-PMF). This material was evidenced by safe in animal toxic study. The main aim of this study was to revise the traditional way of hot water extraction to fermentation so as to use up the original material and finding new activity. We tried to show the new activity through phytoestrogen and immune-competent cells from the host that administrated either of original remedy and the new fermented sample, plus activated water SRE. In mice, compromised host was prepared by cancer chemotherapeutic agent (Mitomycin-C). After administration of f-PMF to immno-suppressed animals, the effects by both samples were augmented by lymphocyte in number and functions, macrophage activities, anti-oxidative activity. However, the intense of effect was much more by fermented one but not by conventional one. The anti-oxidative assay was also carried out ex-vivo system by peritoneal macrophage that we proposed as suitable system for evaluating anti-oxidative assay. In our clinical study by 20 healthy volunteers, granulocyte and lymphocyte ratio was regulated as neutral in peripheral white blood cells, increasing one, two and three weeks after the administration of f-PMF. We have found the significant regulation of blood chemical factors that were important makers for the lifestyle-related diseases. The mechanism of augmentation by probing directory with immuno-electrophoretic method, generating new complement component, especially found by alternative pathway of complement. So we discussed the process concerning designed f-PMF molecule for activation of complement component and bound for the biological activity of each physical component. In a limited condition, fewer numbers of volunteers, the breast size was tending to increase along with the administration time. Including these evidences, we discussed the possibility of this traditional ethnic medicine, originally found and spread in the hi

关键词： Family Pueraria Fermentation Lactobacilli Cancer chemotherapeutic Agent Immune-Compromised Host Lymphocyte Granulocyte Ant-Oxidative Activity Complement Alternative Complement Activation

Fermented Black Turmeric Designed by Lactobacillus Rearranged Leukocyte Subsets and Anti-Oxidative Activity

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Open Journal of Immunology 2015年第3期5卷 199-214页

作者： Yousuke Watanabe Nobuo Yamaguchi Isao Horiuch Tsugia Murayama Ishikawa Natural Medicinal Products Research Center Ishikawa Japan Department of Fundament Research for CAM Kanazawa Medical University Ishikawa Japan Traditional Uighur Medicine Department Xinjiang Medical University Urumchi China Traditional Chinese Medicine Hospital Xinjiang Medical University Urumchi China Faculty of Pharmaceutical Sciences Department of Microbiology and Immunology Hokuriku University Ishikawa Japan

A plant fermentation was carried out by Yeast and Lactobaccilli against fermented black turmeric, Kaempferia parviflora (FBT). These materials were proved by as safe in animal safety experiment. We tried to investigate changes of immune-competent cells that commonly utilized FBT, including after administration of immno-suppressed animals, the effects by FBT on the regulated effect on the cells were evaluated. Our results showed that FBT augmented the level of lymphocytes in number, while FBT regulated the level of granulocytes in both number and function. In our clinical study with 20 healthy volunteers, granulocyte and lymphocyte ratio suggesting their constitution as neutral in peripheral blood were increased significantly 30 days after the administration of FBT in rodents, and compromised host was prepared with cancer chemotherapeutic agent (Mytomycin-C). Our observations showed against intracellular parasite, and that FBT augmented intercellular pathogen through humoral immunity. We discussed the significance and mechanism of FBT on the level of leukocyte subsets in number and function that were considered to be potential indicators for the activation of the compromised host. We also proposed an idea that FBT exhibited tonic effects via activating complement components. The evidences were shown by immune-electrophoretic method. Moreover, we tried to access further to the anti-oxidative activities of this FBT. This modification brought to the significant lift up for antibody producing cells and anti-oxidative activity for phagocytic cells.

关键词： Family Turmeric Lactobacillus Fermentation Lactobacilli Cancer chemotherapeutic Agent Compromised Host Complement Alternative Complement Activation

Studies on the Antibacterial Activity of Ceftiofur Sodium in Vitro and Birds

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Open Journal of Veterinary Medicine 2013年第1期3卷 16-21页

作者： Khalid A. Al-Kheraije Shaqra University Shaqra KSA

This study was carried out to evaluate the antibacterial activity of ceftiofur sodium as anti-infective chemotherapeutic agent belonged to the third generation of cephalosporins against different bacterial pathogens in vitro and birds. The obtained results showed that it was more effective and superior in its action than that the other compared antibacterial agents. The disc diffusion test revealed that most P. multocida isolates were highly sensitive to ceftiofur sodium with minimum inhibitory concentrations (MICs) ranging between 0.625 - 2.5 μg/ml and minimal bactericidal concentration (MBCs) equal or two folds of the MICs.

关键词： Ceftiofur Sodium (Excenel) Microorganisms Minimum Inhibitory Concentration (MICs) Minimum Bactericidal Concentration (MBCs) chemotherapeutic Birds

Toxicological Evaluation of Occidiofungin against Mice and Human Cancer Cell Lines

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Pharmacology & Pharmacy 2014年第11期5卷 1085-1093页

作者： Steven Lai Hing Akshaya Ravichandran Jerome Escano Jim Cooley Frank Austin Shi-En Lu Stephen Pruett Leif Smith Department of Biological Sciences Texas A&M University College Station USA Department of Pathobiology and Population Medicine College of Veterinary Medicine Mississippi State University USA Department of Biochemistry Molecular Biology Entomology and Plant Pathology Mississippi State University USA Department of Basic Sciences College of Veterinary Medicine Mississippi State University USA

Occidiofungin, a glyco-lipopeptide obtained from the liquid culture of Burkholderia contaminans MS14, has been identified as a novel fungicide. The natural product was shown to have a minimal amount of toxicity in a previous mouse toxicity study following intraperitoneal and subcutaneous administration. In this study, the toxicity of occidiofungin was evaluated following a 5 mg/kg intravenous tail vein injection. In addition, the toxicity of occidiofungin was evaluated against human fibroblast and cancer cell lines. Weight loss was the most significant observation following intravenous administration of occidiofungin. Histology, hematology, and blood serum chemistry did not reveal any significant signs of toxicity. The activity observed in the in vitro cytotoxicity assay against the cancer cell lines was all below 75 nM concentration of occidiofungin. To date, the potency of occidiofungin against these cancer cell lines is greater than any activity observed against fungi. The findings in this study support the need to further evaluate occidiofungin’s chemotherapeutic potential.

关键词： Antifungal chemotherapeutic Toxicity Histopathology