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Site-specific quantification of 5-carboxylcytosine in DNA by chemical conversion coupled with ligation-based PCR

Chinese chemical Letters 2021年第11期32卷 3426-3430页

作者： Qian Wang Jiang-Hui Ding Jun Xiong Yang Feng Bi-Feng Yuan Yu-Qi Feng Sauvage Center for Molecular Sciences Department of ChemistryWuhan UniversityWuhan 430072China School of Health Sciences Wuhan UniversityWuhan 430071China

5-Methylcytosine(5mC)is the most important epigenetic modification in *** active DNA demethylation could be achieved through the ten-eleven translocation(TET)protein-mediated oxidization of 5mC with the generation of 5-hydroxymethylcytosine(5hmC),5-formylcytosine(5fC)and 5-carboxylcytosine(5caC).It has been known that 5mC,5hmC and 5fC play critical roles in modulating gene ***,unlike the 5mC,5hmC,and 5fC,the functions of 5caC are still *** of the functions of 5caC relies on the accurate quantification and localization analysis of 5caC in *** the current study,we developed a method by chemical conversion in conjugation with ligation-based real-time quantitative PCR(qPCR)for the site-specific quantification of 5caC in *** method depends on the selective conversion of 5caC to form dihydrouracil(DHU)by pyridine borane *** behaves like thymine and pairs with adenine(DHU-A).Thus,the chemical conversion by pyridine borane leads to the transformation of base paring from 5caC-G to DHU-A,which is utilized to achieve the site-specific detection and quantification of 5caC in *** a proof-of-concept,the developed method was successfully applied in the site-specific quantification of 5caC in synthesized DNA spiked in complex biological *** method is rapid,straightforward and cost-effective,and shows promising in promoting the investigation of the functional roles of 5caC in future study.

关键词： 5-Methylcytosine 5-Carboxylcytidine Dihydrouracil Mass spectrometry PCR chemical conversion

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A New Porous MOF with Highly Efficient Selectivity and chemical conversion for CO2

A New Porous MOF with Highly Efficient Selectivity and Chemi...

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中国化学会第八届全国配位化学会议

作者： Hai-Hua Wang Lei Hou Yao-Yu Wang College of Chemistry & Materials Science Northwest University

Porous MOFs due to endowed designable structures and facile pore functionalization can be designed to efficiently convert CO into valuable chemicals.[1,2]The combining advantages of adsorbent and heterogeneous catalysis ensure MOFs to be excellent candidates to meet the dual requires of capture and conversion of CO.A new Co(Ⅱ)-based MOF,{[Co(tzpa)(OH)(H O)]·DMF}(1)(H tzpa =5-(4-(tetrazol-5-yl)phenyl)isophthalic acid) was constructed by employing a tetrazolylcarboxyl ligand H tzpa.1 possesses 1 D tubular channels that are decorated by μ-OH groups,uncoordinated carboxylate O atoms and open metal centers generated by the removal of coordinated water molecules,leading to high CO adsorption capacity in the temperature range of298-333 ***,1 shows the moderately chemical stability in acidic and basic aqueous *** addition,the activated 1 as the heterogeneous Lewis&Bronsted acid bifunctional catalyst facilitates the chemical fixation of CO coupling with epoxides into cyclic carbonates under the ambient condition.

关键词： Metal-organic framework CO2 adsorption selectivity chemical conversion

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Structures,chemical conversions,and Cytotoxicity of Tricholopardins C and D,Two Tricholoma Triterpenoids from the Wild Mushroom Tricholoma pardinum

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Natural Products and Bioprospecting 2021年第2期11卷 235-241页

作者： Chen Shi Yue‑Ling Peng Juan He Zheng‑Hui Li Ji‑Kai Liu Tao Feng School of Pharmaceutical Sciences South-Central University for NationalitiesWuhan 430074People’s Republic of China The Third People’s Hospital of Datong Datong 037000People’s Republic of China

Two undescribed Tricholoma triterpenoids,namely tricholopardins C(1)and D(2),were isolated from the wild mushroom Tricholoma *** structures with absolute configurations were elucidated by spectroscopic methods,as well as the single crystal X-ray *** 1 and 2 were further obtained by chemical conversions from the known *** 1 showed significant cytotoxicity to MCF-7 and Hela cell lines with IC_(50)values of 4.7μM and 9.7μM,*** mechanism of inducing MCF-7 cell apoptosis was studied briefly.

关键词： Tricholoma pardinum Tricholoma triterpenoids chemical conversion Cytotoxicity

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ZnO/ZnS core-shell composites for low-temperature-processed perovskite solar cells

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Journal of Energy Chemistry 2018年第5期27卷 1461-1467页

作者： Enqiang Zheng Yaqin Wang Jiaxing song Xiao-Feng Wang Wenjing Tian Gang Chen Tsutomu Miyasaka Key Laboratory of Physics and Technology for Advanced Batteries (Ministry of Education) College of Physics Jilin University Changchun 130012 Jilin China State Key Laboratory of Supramolecular Structure and Materials. Jilin University Changchun 130012 Jilin China Graduate School of Engineering Toin University of Yokohama 1614 Kurogane-cho Aoba Yokohama Kanagawa 225-8503 Japan

Electron transport layers （ETLs） in perovskite solar cells （PSCs） are a key factor to determine the photo- voltaic performance. Herein, we demonstrate preparation of ZnO/ZnS core-shell composites through di- rectly synthesizing ZnS on the ZnO nanoparticles in solution. We confirmed the formation of ZnO/ZnS core-shell composites by the uses of X-ray diffraction patterns and the Fourier transform infrared spec- troscopy. ZnO/ZnS composites exhibit much homogeneous surface morphology as compared with the bare ZnO as revealed in the scanning electronic microscopy. Moreover, the upper shift of conduction band level upon composition of the ZnO/ZnS film results in a better alignment of energy level, which facilitates cas- cade charge extraction and thus improves the current density of perovskite solar cell. The shift of conduc- tion band also improves the voltage of the PSCs. The photoluminescence （PL） spectroscopies measured in both steady and transient states were carried out to characterize the charge extraction at the interface between CH_3NH_3Pbl_3 and the electron transport layers of either ZnO or ZnO/ZnS composite. The ZnO/ZnS composite can more efficiently quench the PL signal of perovskite absorber than bare ZnO resulting in enhanced photocurrent generation in PSCs.

关键词： ZnO/ZnS Electron transport layer Low-temperature-processed chemical conversion Perovskite solar cells

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Rare Earth conversion Coating of Magnesium Alloy AZ91D

Rare Earth Conversion Coating of Magnesium Alloy AZ91D

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Second International Conference on Magnesium(ICM 2006)

作者： Liqun BAI, Di LI, Min GUO and Jing XIN School of Materials Science and Engineering, Beijing University of Aeronautics and Astronautics, Beijing 100083, China Chinese people armed police academy, Langfang 102849, Hebei, China

Rare earth chemical conversion coating of Mg alloys was studied. Corrosion and electrochemical behavior in chloride environment were investigated with tests of evolution of hydrogen and electrochemical measurements. The surface morphologies and composition of rare earth conversion coating were studied through SEM, EDAX and XPS. The results showed that rare earth conversion coatings could improve corrosion resistance and their corrosion resistance was comparable with that of chromate coatings (HB/Z5078278). This result was further proved by Polarization and EIS.

关键词： AZ91D alloy rare earth chemical conversion corrosion resistance

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