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Effects of Acupuncture at Baihui(GV 20) and Zusanli(ST 36) on Peripheral Serum Expression of MicroRNA 124,Laminin and Integrin β1 in Rats with cerebral ischemia reperfusion injury

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Chinese Journal of Integrative Medicine 2016年第1期22卷 49-55页

作者：陈素辉孙华张亚敏徐虹杨杨王富明 Department of Traditional Chinese Medicine Peking Union Medical College Hospital Peking Union Medical CollegeChinese Academy of Medical Sciences

Objective： To explore the effects of acupuncture at Baihui （GV 20） and Zusanli （ST 36） on the peripheral serum expression of microRNA 124 （miRNA 124）, laminin and integrinβ 1 in rats with cerebral ischemia reperfusion injury （CIRI）. Methods： Seventy-two healthy male Sprague-Dawley rats were randomized into a model group, an acupuncture group, and a sham-operated group using a random digits table, with 24 rats per group. Each group was further randomly divided into 1-, 3-, 5-, and 7-day subgroups based on the reperfusion time according to a random digits table, with 6 rats in each subgroup. In the model and acupuncture groups, CIRI was induced using the thread occlusion method. Electroacupuncture stimulation was applied daily to GV 20 and left ST 36 for 20 min at the indicated time points after successful operations. Serum was sampled for detecting laminin and integrin β 1 protein via enzyme-linked immunosorbent assay, and serum miRNA 124 was examined using quantitative polymerase chain reaction. Results： The serum level of miRNA 124 in the cerebral ischemia rats increased significantly, and the peak expression of miRNA 124 in both the model and acupuncture groups occurred at 3 days. The expression of miRNA 124 in the acupuncture group was higher than in the model group at the same time point （5.96±0.01 vs. 3.11 ±0.04, P〈0.05）. Laminin expression in serum from the cerebral ischemia group was higher than that in the sham-operated group. Compared with the model group, the level of laminin in the serum of the acupuncture group was significantly lower at each time point, especially at the 3-day, and 7-day time points （589.12± 3.57 vs. 793.05± 5.28, and 600.53± 3.05 vs. 899.06± 5.74, P〈0.05）. The level of integrin 131 in the serum from the acupuncture group was lower than that in the model group particularly at the 3-day and Z-day time points （208.66± 0.95 vs. 280.83± 1.77, and 212.36±0.95 vs. 316.77±2.42, P〈0.05）. Additionally, the model group and the acupuncture group showed dual peaks of integrin β 1 and laminin exp

关键词： acupuncture cerebral ischemia reperfusion injury microRNA 124 laminin integrin β 1

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Neuroprotective effects of a mitochondrial K^+-ATP channel opener (diazoxide) are mediated by Bcl-2 expression upregulation

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Neural Regeneration Research 2011年第12期6卷 956-960页

作者： Majid Katebi Mansooreh Soleimani Mehdi Mehdizadeh Department of Anatomy School of MedicineHormozgan University of Medical Sciences Cellular and Molecular Research Center Tehran University of Medical Sciences Department of Anatomy School of MedicineTehran University of Medical Sciences Research Institute for Islamic & Complementary Medicine Tehran University of Medical Sciences

Mitochondrial K＋-ATP （mito-KATP） channels play an important role in cellular function and survival following ischemic stress. The present results revealed that intervention with diazoxide, a mito-KATP channel opener, led to an increase in Bcl-2 expression in the cerebral cortex of rats subjected to cerebral ischemia reperfusion injury. In addition, the intervention also led to clear improvements in neuronal mitochondrial morphology and consciousness post-injury. Glibenclamide a mito-KATP channel blocker, exhibited the converse effects. Both diazoxide and glibenclamide exerted dose-dependent effects （in particular, at 18 mg/kg diazoxide and 25 mg/kg glibenclamide）. These findings suggest that diazoxide exerts a neuroprotective effect on cerebral ischemia reperfusion injury by opening mito-KATP channels and upregulating Bcl-2 expression.

关键词： K-ATP channel Bcl-2 cerebral ischemia reperfusion injury diazoxide

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