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The relationship between the c-jun mRNA expression and apoptosis of neurons in rat brain following perinatal ischemic-hypoxia

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Chinese Medical Journal 1999年第1期112卷 41-44页

作者：母得志梁卫兰张国荣吴希如 China 100034

Objective To investigate the relationship between c jun mRNA and apoptosis of neurons following perinatal ischemic hypoxia. Methods We set up a fetal rat model of perinatal ischemic hypoxia by ligating unilateral uterine horn vessel of pregnant Wistar rats (21 day gestation). The contralateral horn vessel was not ligated and the fetuses in these uteri served as controls. Rat pups were delivered by cesarean section at the end of ischemic hypoxic insult and then the rats' brain tissues were collected in different points of time. In situ hybridization and TUNEL methods were used to detect the c jun mRNA expression and neural cells apoptosis separately. Results The expression of c jun mRNA in brain tissues began at 15 minutes, reached the first peak at 1-2 hours after ischemic hypoxic insult and reduced gradually after 4 hours and the second peak at 24th hour after insult, gradually disappeared till 72nd hour. There were very low c jun mRNA expression of hippocampus in the control group 24 h after birth. Meanwhile, we observed that the apoptosis of neuronal cells in cerebral cortex was much more than that in the control group. Conclusion The increases of immediate early gene c jun expression and the cell apoptosis could be induced by perinatal ischemic hypoxia. The higher expression of c jun mRNA might induce the transcription of its target gene, especially, so called “death related genes”, which would be a promoter for cell apoptosis.

关键词： cerebral ischemia cerebral hypoxia perinatology gene apoptosis

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Early application of nerve growth factor affects serum inflammatory cytokine levels in neonatal hypoxic ischemic encephalopathy

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Neural Regeneration Research 2007年第7期2卷 437-439页

作者： Jingguo Chen Huijuan Huang Kaijun Zheng Ronghua Xia Jian Chen Xiufang Yang Chunhua Ye Qiang Lin Department of Neonatology Sun Yat-sen Hospital of Sun Yat-sen University Zhongshan 528403 Guangdong Province China

BACKGROUND： It has been demonstrated that there are changes of various cytokines, chemokines and adhesion factors in neonatal hypoxic ischemic encephalopathy （HIE）. What are the changes of interleukin-6 and interleukin-18 in serum of HIE neonates.OBJECTIVE： To observe the dynamic changes of interleukin-6 and interleukin-18 in peripheral serum at different time after HIE in neonates, and analyze the possible therapeutic efficacy of early application of NGF.DESIGN： A non-randomized controlled observation synchronically.SETTING： Department of Neonatology, Sun Yat-sen Hospital affiliated to Sun Yat-sen University.PARTICIPANTS： Sixty neonates with HIE were selected from the Department of Neonatology, Sun Yat-sen Hospital affiliated to Sun Yat-sen University from January 2004 to October 2006, including 32 boys and 28 girls, who were all accorded with the diagnostic standards for moderate to severe HIE. The neonates were divided into two groups NGF-treated group （n =30）, HIE group （n =30）. The HIE neonates in the NGF-treated group were given routine treatment and intramuscular injection of NGF within 24 hours after birth. Those in the HIE group were given routine treatments. Meanwhile, 30 apneic normal neonates （17 boys and 13 girls） at the same period were selected as the control group. The gestational age was 37 - 42 weeks in all the three groups, the body mass at birth was 2 500 - 4 000 g. Informed contents were obtained from the relatives of all the enrolled neonates.METHODS： The HIE neonates in the NGF-treated group were given routine treatment and intramuscular injection of NGF （2 000 U） within 24 hours after birth, once a day, 10 days as a course. Those in the HIE group were given routine treatments. Blood samples （3 mL） were drawn from femoral vein in all the neonates 1, 3 and 7 days after birth. The levels of interleukin-6 and interleukin-18 in serum were detected with enzyme-linked immunoabsorbent assay （ELISA）.MAIN OUTCOME MEASURES： Serum levels of i

关键词： cerebral hypoxia ischemia brain nerve growth factor interleukin-6 interleukin-18

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