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A Global Multiregional Proteomic Map of the Human cerebral cortex

Genomics, Proteomics & Bioinformatics 2022年第4期20卷 614-632页

作者： Zhengguang Guo Chen Shao Yang Zhang Wenying Qiu Wenting Li Weimin Zhu Qian Yang Yin Huang Lili Pan Yuepan Dong Haidan Sun Xiaoping Xiao Wei Sun Chao Ma Liwei Zhang Core Facility of Instrument Institute of Basic Medical SciencesChinese Academy of Medical SciencesSchool of Basic MedicinePeking Union Medical CollegeBeijing 100005China State Key Laboratory of Proteomics Beijing Proteome Research CenterNational Center for Protein Sciences(Beijing)Beijing Institute of LifeomicsBeijing 102206China Department of Neurosurgery China National Clinical Research Center for Neurological DiseasesBeijing Tiantan HospitalCapital Medical UniversityBeijing 100050China Department of Human Anatomy Histologyand EmbryologyInstitute of Basic Medical SciencesNeuroscience CenterChinese Academy of Medical SciencesSchool of Basic MedicinePeking Union Medical CollegeBeijing 100005China

The Brodmann area(BA)-based map is one of the most widely used cortical maps for studies of human brain functions and in clinical practice;however,the molecular architecture of BAs remains *** present study provided a global multiregional proteomic map of the human cerebral cortex by analyzing 29 *** 29 BAs were grouped into 6 clusters based on similarities in proteomic patterns:the motor and sensory cluster,vision cluster,auditory and Broca’s area cluster,Wernicke’s area cluster,cingulate cortex cluster,and heterogeneous function *** identified 474 cluster-specific and 134 BA-specific signature proteins whose functions are closely associated with specialized functions and disease vulnerability of the corresponding cluster or *** findings of the present study could provide explanations for the functional connections between the anterior cingulate cortex and sensorimotor cortex and for anxiety-related function in the sensorimotor *** brain transcriptome and proteome comparison indicates that they both could reflect the function of cerebral cortex,but show different *** proteomic data are publicly available at the Human Brain Proteome Atlas(***).Our results may enhance our understanding of the molecular basis of brain functions and provide an important resource to support human brain research.

关键词： Brodmann area Proteomics cerebral cortex Cingulate cortex Sensorimotor cortex

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MicroRNA and mRNA profiling of cerebral cortex in a transgenic mouse model of Alzheimer’s disease by RNA sequencing

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Neural Regeneration Research 2021年第10期16卷 2099-2108页

作者： Li Zeng Hai-Lun Jiang Ghulam Md Ashraf Zhuo-Rong Li Rui Liu Organic Chemistry and Function Laboratory Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina King Fahd Medical Research Center King Abdulaziz UniversityJeddahSaudi Arabia Department of Medical Laboratory Technology Faculty of Applied Medical SciencesKing Abdulaziz UniversityJeddahSaudi Arabia

In a previous study,we found that long non-coding genes in Alzheimer’s disease(AD)are a result of endogenous gene disorders caused by the recruitment of microRNA(miRNA)and mRNA,and that miR-200a-3p and other representative miRNAs can mediate cognitive impairment and thus serve as new biomarkers for *** this study,we investigated the abnormal expression of miRNA and mRNA and the pathogenesis of AD at the epigenetic *** this aim,we performed RNA sequencing and an integrative analysis of the cerebral cortex of the widely used amyloid precursor protein and presenilin-1 double transgenic mouse model of ***,129 mRNAs and 68 miRNAs were aberrantly *** these,eight down-regulated miRNAs and seven up-regulated miRNAs appeared as promising noninvasive biomarkers and therapeutic *** main enriched signaling pathways involved mitogen-activated kinase protein,phosphatidylinositol 3-kinase-protein kinase B,mechanistic target of rapamycin kinase,forkhead box O,and *** miRNA-mRNA network between dysregulated miRNAs and corresponding target genes connected with AD progression was also *** miRNAs and mRNAs are potential biomarkers and therapeutic targets for new treatment strategies,early diagnosis,and prevention of *** present results provide a novel perspective on the role of miRNAs and mRNAs in *** study was approved by the Experimental Animal Care and Use Committee of Institute of Medicinal Biotechnology of Beijing,China(approval ***-201909-D6)on September 6,2019.

关键词： 3ʹ-untranslated region Alzheimer’s disease biomarker cerebral cortex Gene Ontology high-throughput sequencing intracellular neurofibrillary tangles microtubule-associated protein-τ miRNA-mRNA network presenilin 1

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Expression of estrogen receptor （ER） -α and -βtranscripts in the neonatal and adult rat cerebral cortex, cerebellum, and olfactory bulb

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Cell Research 2001年第4期11卷 321-324页

作者： GUO XING ZHONG, JUN DA SU, QING WEN SUN, BING HUA JIAO Department of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai 200433 School of Life Sciences and Medical Engineering, Tongji University, Shanghai 200092, China Shangha 1Department of Biochemistry and Molecular Biology Second Military Medical University Shanghai 200433 3Shanghai Institute of Physiology Chinese Academy of Sciences Shanghai 200031 China 1Department of Biochemistry and Molecular Biology Second Military Medical University Shanghai 200433 1Department of Biochemistry and Molecular Biology Second Military Medical University Shanghai 200433

In the present study expression of estrogen receptor subtype -α(ERα) and -β (ERβ) in the cerebral cortex, cerebellum, and olfactory bulb was investigated and compared between neonatal (1～3-days-old) and adult (250～350g) rats, using reverse transcription-polymerase chain reaction (RT- PCR). No ERα transcripts were detectable in the adult cerebellum and olfactory bulb, whereas very weak expression of ERα was present in the adult cerebral cortex. No significant difference in ERβ transcripts was detectable between the neonatal and adult rats. While transcripts for both ER subtypes were co-expressed in these brain areas of neonatal rats, although ERα expression was significantly weaker than ERβ. Even in the cerebral cortex known to contain both ER subtypes in adult rats, ERα transcripts in neonatal rats were much higher than in adult. These observations provide evidence for the existence of different expression patterns of ERcα/ERβ transcripts in these three brain areas between the neonatal and adult rats, suggesting that each ER subtype may play a distinct role in the regulation of differentiation, development, and functions of the brain by estrogen.

关键词： Cerebellum cerebral cortex estrogen receptor (ER) olfactory bulb RT-PCR.

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Causal interactions between the cerebral cortex and the autonomic nervous system

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Science China(Life Sciences) 2014年第5期57卷 532-538页

作者： YU XiaoLin ZHANG Chong ZHANG JianBao Department of Information Engineering Officers College of Chinese People's Armed Police Force Key Laboratory of Biomedical Information Engineering of Ministry of Education Xi'an Jiaotong University

Mental states such as stress and anxiety can cause heart *** the other hand,meditation can improve cardiac *** this study,the heart rate variability,directed transfer function and corrected conditional entropy were used to investigate the effects of mental tasks on cardiac performance,and the functional coupling between the cerebral cortex and the *** subjects tried to decrease their heart rate by volition,the sympathetic nervous system was inhibited and the heart rate *** subjects tried to increase their heart rate by volition,the parasympathetic nervous system was inhibited and the sympathetic nervous system was stimulated,and the heart rate *** autonomic nervous system activity was regulated by mental tasks,the information flow from the post-central areas to the pre-central areas of the cerebral cortex increased,and there was greater coupling between the brain and the *** of directed transfer function and corrected conditional entropy techniques enabled analysis of electroencephalographic recordings,and of the information flow causing functional coupling between the brain and the heart.

关键词： mental task autonomic nervous system cerebral cortex heart rate variability information flow

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Expression of bone morphogenetic protein 7 in the cerebral cortex of rats after ischemic-hypoxic injury

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Neural Regeneration Research 2007年第11期2卷 641-644页

作者： Liju Luan Xiaomei Yang Lihua Qin Ke Wang Chunhua Chen Weiguang Zhang Lu Wan Dan Li Department of Anatomy and Histoembryology Peking University Health Science Center Beijing 100083 China

BACKGROUND： Some researches demonstrate that exogenous bone morphogenetic protein 7 （BMP-7） can protect ischemic cerebral nerve tissue and promote recovery of motor energy function; however, there is lack of direct evidences of endogenous BMP-7 effect. OBJECTIVE： To observe the expression of endogenous BMP-7 in nerve tissue with ischemic-hypoxic injury and investigate the possible effects on damaged nerve tissue. DESIGN： Observational contrast animal study. SETTING： Department of Anatomy and Histoembryology, Peking University Health Science Center. MATERIALS： The experiment was carried out in the Nerve Researching Laboratory of Anatomy Department, Peking University Health Science Center from October 2006 to March 2007. A total of 25 adult male SD rats weighing 250 - 300 g and several newborn SD rats were selected from Experimental Animal Center, Peking University Health Science Center. Rabbit-anti-BMP-7 polyclonal antibody was provided by Wuhan Boster Company. METHODS： ① Adult rats were randomly divided into ischemia group （n =10）, sham operation group （n = 10） and normal group （n =5）. Right external-internal carotid artery occlusion was used to infarct middle cerebral artery of adult rats in the ischemia group so as to copy focal cerebral infarction models. Line cork was inserted in crotch of internal and external carotid artery of adult rats in the sham operation group, while adult rats in the normal group were not given any treatments. ② cerebral cortex of newborn rats was separated to obtain cell suspension. Cells which were cultured for 10 days were divided into control group and hypoxia/reoxygenation group. And then, cells in the hypoxia/reoxygenation group were cultured in hypoxic incubator for 4 hours and given reoxygenation for 24 hours. MAIN OUTCOME MEASURES： Immunohistochemical method was used to measure expression of BMP-7 in cerebral cortex at 24 hours after ischemia/reperfusion culture and in primary hypoxic culture. RESULTS： ① At 24 hours

关键词： cerebral ischemia hypoxia bone morphogenetic protein 7 cerebral cortex rats

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Agmatine reduces nitric oxide synthase expression and peroxynitrite formation in the cerebral cortex in a rat model of transient global cerebral ischemia

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Neural Regeneration Research 2010年第23期5卷 1773-1781页

作者： Chin Hee Mun Won Taek Lee Kyung Ah Park Jong Eun Lee Department of Anatomy Yonsei University College of Medicine Brain Korea 21 Project for Medical Science Yonsei University College of Medicine

Agmatine, an analog of L-arginine, is an endogenous substance synthesized by arginine decarboxylase, which has been shown to possess neuroprotective effects following brain ischemia. Nitric oxide is generated by sequential oxidation of the guanidinium group in L-arginine, and agmatine might protect the brain from ischemic injury by interfering with nitric oxide signaling. This study investigated the effects of agmatine on cerebral cortex neuronal injury following transient global cerebral ischemia and also detected nitric oxide synthase expression and peroxynitrite formation. Results demonstrated that intraperitoneal injection of agmatine in global cerebral ischemia/reperfusion alleviated ischemia/reperfusion-induced cerebral cortical cortex neuronal injury and cellular apoptosis, decreased neuronal and inducible nitric oxide synthase expression at 24, 48, and 72 hours following global cerebral ischemia and reperfusion, and greatly inhibited nitrotyrosine levels, which reflect the amount of peroxynitrite formed. These findings indicated that agmatine alleviates cerebral cortex neuronal injury following global cerebral ischemia and decreases nitric oxide synthase expression and peroxynitrite formation following ischemia/repeffusion.

关键词： agmatine global cerebral ischemia cerebral cortex nitric oxide nitric oxide synthase neuroprotection

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Electroacupuncture stimulation of the brachial plexus trunk on the healthy side promotes brain-derived neurotrophic factor mRNA expression in the ischemic cerebral cortex of a rat model of cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2012年第21期7卷 1618-1623页

作者： Zongjun Guo Lumin Wang Department of Special Health Care Affiliated Hospital of Qingdao University Medical College Qingdao 266003 Shandong Province China Department of Emergency Treatment Affiliated Hospital of Qingdao University Medical College Qingdao 266003 Shandong ProvinceChina

A rat model of cerebral ischemia/reperfusion was established by suture occlusion of the left middle cerebral artery. In situ hybridization results showed that the number of brain-derived neurotrophic factor mRNA-positive cells in the ischemic rat cerebral cortex increased after cerebral ischemia/ reperfusion injury. Low frequency continuous wave electroacupuncture （frequency 2-6 Hz, current intensity 2 mA） stimulation of the brachial plexus trunk on the healthy （right） side increased the number of brain-derived neurotrophic factor mRNA-positive cells in the ischemic cerebral cortex 14 days after cerebral ischemia/reperfusion injury. At the same time, electroacupuncture stimulation of the healthy brachial plexus truck significantly decreased neurological function scores and alleviated neurological function deficits. These findings suggest that electroacupuncture stimulation of the brachial plexus trunk on the healthy （right） side can greatly increase brain-derived neurotrophic factor mRNA expression and improve neurological function.

关键词： ischemia/reperfusion brain-derived neurotrophic factor electroacupuncture brachial plexus trunk cerebral cortex in situ hybridization neural regeneration

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Optimal concentration and time window for proliferation and differentiation of neural stem cells from embryonic cerebral cortex: 5% oxygen preconditioning for 72 hours

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Neural Regeneration Research 2015年第9期10卷 1516-1522页

作者： Li-li Yuan Ying-jun Guan Deng-dian Ma Hong-mei Du Department of Histology and Embryology Academy of Basic Medicine Jining Medical University Department of Histology and Embryology Academy of Basic Medicine Weifang Medical University Department of Otorhinolaryngology Affiliated Hospital of Jining Medical University

Hypoxia promotes proliferation and differentiation of neural stem cells from embryonic day 12 rat brain tissue, but the concentration and time of hypoxic preconditioning are controversial. To address this, we cultured neural stem cells isolated from embryonic day 14 rat cerebral cortex in 5% and 10% oxygen in vitro. MTT assay, neurosphere number, and immunofluorescent staining found that 5% or 10% oxygen preconditioning for 72 hours improved neural stem cell viability and proliferation. With prolonged hypoxic duration （120 hours）, the proportion of apoptotic cells increased. Thus, 5% oxygen preconditioning for 72 hours promotes neural stem cell prolif- eration and neuronal differentiation. Our findings indicate that the optimal concentration and duration of hypoxic preconditioning for promoting proliferation and differentiation of neural stem cells from the cerebral cortex are 5% oxygen for 72 hours.

关键词： nerve regeneration brain injury neural stem cells low oxygen cerebral cortex apoptosis differentiation microtubule-associated protein 2 glial fibrillary acidic protein caspase-3 neuralregeneration

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Pine pollen inhibits cell apoptosis-related protein expression in the cerebral cortex of mice with arsenic poisoning

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Neural Regeneration Research 2012年第12期7卷 896-899页

作者： Yanhong Luo Yaodong Wei Taizhong Wang Dongzhu Chen Tiansheng Lu Ruibo Wu Keke Si Department of Clinical Biochemistry and Molecular Biology Youjiang Medical College for Nationafities Baise 533000 Guangxi Zhuang Autonomous Region China Department of Biochemistry and Molecular Biology Youjiang Medical College for Nationalities Baise 533000 Guangxi Zhuang Autonomous Region China Grade 2008 Department of Medical Laboratory Science Youjiang Medical College for Nationalities Baise 533000 Guangxi Zhuang Autonomous Region China

Previous studies have demonstrated that pine pollen can inhibit cerebral cortical cell apoptosis in mice with arsenic poisoning. The present study sought to detect the influence of pine pollen on apoptosis-related proteins. Immunohistochemistry, western blotting and enzyme-linked immunosorbent assays were used to measure the levels of apoptosis-related proteins in the cerebral cortex of mice with arsenic poisoning. Results indicated that pine pollen suppressed cell apoptosis in the cerebral cortex of arsenic-poisoned mice by reducing Bax, Bcl-2 protein expression and increasing p53 protein expression.

关键词： pine pollen arsenic poisoning apoptosis cerebral cortex neural regeneration

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Effects of sericin on heme oxygenase-1 expression in the hippocampus and cerebral cortex of type 2 diabetes mellitus rats

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Neural Regeneration Research 2011年第6期6卷 423-427页

作者： Zhihong Chen Yaqiang He Wenliang Fu Jingfeng Xue Department of Human Anatomy Chengde Medical University Chengde 067000 Hebei Province China

Previous studies have demonstrated that sericin effectively reduces blood glucose, and protects islet cells, as well as the gonads and kidneys. However, whether sericin improves diabetes mellitus-induced structural and functional problems in the central nervous system remains poorly understood. Rat models of type 2 diabetes mellitus were established by intraperitoneal injection of streptozotocin. The present study observed histological changes in the hippocampus and cerebral cortex, as well as heme oxygenase-1 expression, and explored sericin effects on the central nervous system in diabetic rats. Pathological damage to neural cells in the rat hippocampus and cerebral cortex was relieved following intragastric administration of sericin at a dose of 2.4 g/kg for 35 consecutive days. Heme oxygenase-1 protein and mRNA expressions were decreased in the hippocampus and cerebral cortex of diabetes mellitus rats after sericin treatment. The results suggest that sericin plays a protective effect on the nervous system by decreasing the high expression of heme oxygenase-1 following diabetes mellitus.

关键词： sericin diabetes mellitus hippocampus cerebral cortex heme oxygenase-1

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