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Interleukin-1βinduces human cementoblasts to support osteoclastogenesis

International Journal of Oral Science 2017年第4期9卷 216-223页

作者： nam c-n huynh vincent everts prasit pavasant ruchanee s ampornaramveth research unit on oral microbiology and immunology microbiology departmentfaculty of dentistrychulalongkorn universityBangkokThailand mineralized tissue research unit faculty of dentistrychulalongkorn universityBangkokThailand department of dental basic sciences faculty of odonto-stomatologyuniversity of medicine and pharmacy at ho chi minh cityHO Chi Minh CityVietnam department of oral cell biology academic centre for dentistry amsterdam(acta)university of amsterdam and vu university amsterdamThe Netherlands department of anatomy faculty of dentistrychulalongkorn universityBangkokThailand

Injury of the periodontium followed by inflammatory response often leads to mot resorption. Resorption is accomplished by osteoclasts and their generation may depend on an interaction with the cells in direct contact with the root, the cementoblasts. Our study aimed to investigate the role of human cementoblasts in the formation of osteoclasts and the effect of interleukin （IL）- 1β hereupon. Extracted teeth from healthy volunteers were subjected to sequential digestion by type I collagenase and trypsin. The effect of enzymatic digestion on the presence of cells on the root surface was analyzed by histology. Gene expression of primary human cementoblasts （pHCB） was compared with a human cementoblast cell line （HCEM）. The pHCBs were analyzed for their expression of IL-1 receptors as well as of receptor activator of nuclear factor kappa-B ligand （RANKL） and osteoprotegerin （OPG）. In a co-culture system consisting of osteoclast precursors （blood monocytes） and pHCBs, the formation of osteoclasts and their resorptive activity was assessed by osteo-assay and ivory slices. The cells obtained after a 120 min enzyme digestion expressed the highest level of bone sialoprotein, similar to that of HCEM. This fraction of isolated cells also shared a similar expression pattern of IL-1 receptors （ILl-R1 and ILl-R2）. Treatment with IL-11~ potently upregulated RANKL expression but not of OPG. pHCBs were shown to induce the formation of functional osteoclasts. This capacity was significantly stimulated by pretreating the pHCBs with IL-1β prior to their co-culture with human blood monocytes. Our study demonstrated that cementoblasts have the capacity to induce osteoclastogenesis, a capacity strongly promoted by IL-1β. These results may explain why osteoclasts can be formed next to the root of teeth.

关键词： cementoblast interleukin-ll3 osteoclast receptor activator of nuclear factor kappa-B ligand tooth resorption

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