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Effect of microwave radiation combined with cellulase treatment of soybean residue on the culture of Aspergillus oryzae

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Food Bioscience 2022年第6期50卷 84-91页

作者： Huaixiang Tian Yao Liu Li Li Chen Chen Haiyan Yu Xinxin Ma Juan Huang Xinman Lou Haibin Yuan Department of Food Science and Technology Shanghai Institute of TechnologyHaiquan Road 100Shanghai201418China Shanghai Tramy Green Food Co. LtdNo.201Xuanchun RoadSanzao Industrial ParkXuanqiao TownPudong New AreaShanghai201314China

Soybean residue (SR) is a by-product of bean product processing with potential usefulness as a fermentation medium. However, the fermentation efficiency of SR is limited due to its low reducing sugar content. In this study, a method of microwave radiation combined with cellulase hydrolysis (MCH) was developed to increase the reducing sugar content of SR, and the effect of treated SR on fermentation by Aspergillus oryzae was evaluated. First, the process parameters, including the reaction time, pH and temperature, were optimized. Second, the effect of treated SR on fermentation by A. oryzae was evaluated, including changes in cell proliferation and protease viability. The results showed that the MCH method capitalized on the advantages of microwave radiation and cellulase and promoted the enzymatic hydrolysis of fibrous polysaccharides in the crystallization zone of SR. The reducing sugar content of SR significantly increased from 0.42 to 0.98 mg/mL after MCH treatment. In addition, treated SR effectively promoted the proliferation of A. oryzae , and the protease activity reached 111 U/mL after 3 days of fermentation. The results of this study suggested that the MCH method can effectively increase the reducing sugar content of SR to expand its use as a fermentation medium.

关键词： Soybean residue Microwave cellulase hydrolysis Aspergillus oryzae

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Wet Oxidation Pretreatment of Poplar Waste for Enhancing Enzymatic hydrolysis Efficiency

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Paper And Biomaterials 2017年第2期2卷 8-17页

作者： GuiGan Fang ShanShan Liu KuiZhong Shen Yan Lin Institute of Chemical Industry of Forestry Products CAFNational Engineering Lab for Biomass Chemical UtilizationKey Lab of Forest Chemical EngineeringSFAKey Lab of Biomass Energyand Material of JiangsuNanjingJiangsu Province210042China Institute of New Technology of Forestry CAFBeijing100091China Collaboration Innovation Center for High Efficient Processing and Utilization of Forestry Resources Nanjing Forestry UniversityNanjingJiangsu Province210037China Qilu University of Technology Ji'nanShandong Province250353China

In this paper, we described the optimization of the wet oxidation pretreatment conditions to enhance enzymatic hydrolysis efficiency, using poplar waste from the stock section of a paper mill as the raw material. We showed that the optimal conditions of the pretreatment for poplar waste were an initial p H value of 10, a temperature of 195℃, a holding time of 15 min, and an oxygen pressure of 1.2 MPa. In this case, the yield of the obtained solid material produced by the process was 51.7% and the reducing sugar yield was 46.8%. The solid part obtained from the pretreatment process was hydrolyzed by cellulase L-10. The optimal enzymatic conditions were a temperature of 49℃, a duration time of 56 h, an enzyme dosage of 38 FPU/g at a p H value of 4.8, and a solid-to-liquor ratio of 1∶50. The resulting cellulose conversion rate reached 96.4% in terms of the pretreated substances. In addition, a chemical composition analysis of the poplar waste and pretreated material indicated that about 92% of the hemicelluloses and 43% of the lignin in the raw material were degraded and dissolved. In addition, the crystallization decreased from 57.5% to 54.8%. An obvious fibrillation of the fiber pretreated by the wet oxidization process was observed by SEM. Moreover, high-performance liquid chromatography(HPLC) results showed a high xylose content and monosaccharide degradation products in the pretreatment solution. In conclusion, the wet oxidation pretreatment process could efficiently degrade or remove the lignin and hemicellulose, as well as reduce the crystallinity of the lignocellulosic material, which resulted in animprovement of the enzymatic ability and an increase in the cellulose conversion rate.

关键词： poplar waste wet oxidation pretreatment enzymatic hydrolysis efficiency cellulase hydrolysis

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