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Overexpression of cyclooxygenase-2 in human HepG2, Bel-7402 and SMMC-7721 hepatoma cell lines and mechanism of cyclooxygenase-2 selective inhibitor celecoxib-induced cell growth inhibition and apoptosis

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World Journal of Gastroenterology 2005年第40期11卷 6281-6287页

作者： Ning-Bo Liu Tao Peng Chao Pan Yu-Yu Yao Bo Shen Jing Leng Laboratory of Reproductive Medicine Department of Pathology Nanjing MedicalUniversity Nanjing 210029 Jiangsu Province China Atherosclerosis Research Center NanjingMedical U .niversity Nanjing 210029 Jiangsu Province China

AIM： To investigate the cyclooxygenase-2 （COX-2） expression level in human HepG2, Bel-7402 and SMMC-7721 hepatoma cell lines and the molecular mechanism of COX-2 selective inhibitor celecoxib-induced cell growth inhibition and cell apoptosis. METHODS： Hepatoma cells were cultured and treated with celecoxib. Cell in situ hybridization （ISH） and immunocytochemistry were used to detect COX-2 mRNA and protein expression. Proliferating cell nuclear antigen and phosphorylated Akt were also detected by immunocytochemistry assay. Cell growth rates were assessed by 3-（4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium （MTT） bromide colorimetric assay. Celecoxib- induced cell apoptosis was measured by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） and flow cytometry （FCM）. The phosphorylated Akt and activated fragments of caspase-9, caspase-3 were examined by Western blotting analysis. RESULTS： Increased COX-2 mRNA and protein expression were detected in all three hepatoma cell lines. Celecoxib could significantly inhibit cell growth and the inhibitory effect was in a dose- and time-dependent manner evidenced by MTT assays and morphological changes. The apoptotic index measured by TUNEL increased correspondingly with the increased concentration of celecoxib and the reaction time. With 50 μmol/L celecoxib treatment for 24 h, the apoptotic index of HepG2, BEL-7402 and SMMC-7721 cells was 25.01±3.08%, 26.40±3.05%,and 30.60±2.89%, respectively. Western blotting analysis showed remarkable activation of caspase-9, caspase-3 and dephosphorylation of Akt （Thr^308）. Immunocytochemistry also showed the reduction of PCNA expression and phosphorylation Akt （Thr^308） after treatment with celecoxib. CONCLUSION： COX-2 mRNA and protein overexpression in HepG2, Bel-7402 and SMMC-7721 cell lines correlate with the increased cell growth rate. Celecoxib can inhibit proliferation and induce apoptosis of hepatoma cell strains in a dose- and time-dependent manner.

关键词： Apoptosis Akt Celecoxib Caspase cellproliferation COX-2 HCC PCNA

Regulation of the pentose phosphate pathway in cancer

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Protein & Cell 2014年第8期5卷 592-602页

作者： Peng Jiang Wenjing Du Mian Wu School of Life Sciences Tsinghua University Beijing 100084 China Hefei National Laboratory for Physical Sciences at Microscale School of Life Sciences University of Science and Technology of China Hefei Anhui 230027 China Department of Cancer Biology Perelman School of Medicine Abramson Family Cancer Research Institute University of Pennsylvania Philadelphia PA 19104 USA

Energy metabolism is significantly reprogrammed in many human cancers, and these alterations confer many advantages to cancer cells, including the pro- motion of biosynthesis, ATP generation, detoxification and support of rapid proliferation. The pentose phos- phate pathway （PPP） is a major pathway for glucose catabolism. The PPP directs glucose flux to its oxi- dative branch and produces a reduced form of nico- tinamide adenine dinucleotide phosphate （NADPH）, an essential reductant in anabolic processes. It has become clear that the PPP plays a critical role in regulating cancer cell growth by supplying cells with not only ribose-5-phosphate but also NADPH for detoxification of intracellular reactive oxygen species, reductive biosynthesis and ribose biogenesis. Thus, alteration of the PPP contributes directly to cell pro- liferation, survival and senescence. Furthermore, recent studies have shown that the PPP is regulated oncogenically and/or metabolically by numerous fac- tors, including tumor suppressors, oncoproteins and intracellular metabolites. Dysregulation of PPP flux dramatically impacts cancer growth and survival. Therefore, a better understanding of how the PPP is reprogrammed and the mechanism underlying the balance between glycolysis and PPP flux in cancer will be valuable in developing therapeutic strategies targeting this pathway.

关键词： pentose phosphate pathway (PPP)G6PD NADPH glucose metabolism cancer cellproliferation

Glehnia fittoralis Extract Promotes Neurogenesis in the Hippocampal Dentate Gyrus of the Adult Mouse through Increasing Expressions of Brain-Derived Neurotrophic Factor and Tropomyosin-Related Kinase B

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Chinese Medical Journal 2018年第6期131卷 689-695页

作者： Joon Ha Park Bich Na Shin Ji Hyeon Ahn Jeong Hwi Cho Tae-Kyeong Lee Jae-Chul Lee Yong Hwan Jeon II Jun Kang Ki-Yeon Yoo In Koo Hwang Choong Hyun Lee Yoo Hun Noh Sung-Su Kim Moo-Ho Won Jong Dai Kim Department of Biomedical Science and Research Institute for Bioscience and Biotechnology Hallym University Chuncheon 24252 Korea Department of Neurobiology School of Medicine Kangwon National University Chuncheon 24341 Korea Department of Radiology School of Medicine Kangwon National University Chuncheon 24341 Korea Department of Food Science and Nutrition Hallym University Chuncheon 24252 Korea epartment of Oral Anatomy College of Dentistry and Research Institute of Oral Biology Gangneung-Wonju National University Gangneung 25457 Korea Department of Anatomy and Cell Biology College of Veterinary Medicine and Research Institute for Veterinary Science Seoul National University Seou108826 Korea Department of Pharmacy College of Pharmacy Dankook University Cheonan 31116 Korea Famenity Biomedical Research Center Famenity Inc. Gyeonggi 13837 Korea Division of Food Biotechnology School of Biotechnology Kangwon National University Chuncheon 24341 Korea

Background： Glehnia littoralis has been used for traditional Asian medicine, which has diverse therapeutic activities. However, studies regarding neurogenic effects of G. littoralis have not yet been considered. Therefore, in this study, we examined effects of G. littoralis extract on cell proliferation, neuroblast differentiation, and the maturation of newborn neurons in the hippocampus of adult mice. Methods： A total of 39 male ICR mice （12 weeks old） were randomly assigned to vehicle-treated and 100 and 200 mg/kg G. littoralis extract-treated groups （n = 13 in each group）. Vehicle and G. littoralis extract were orally administrated for 28 days. To examine neurogenic effects ofG. litmralis extract, we performed immunohistochemistry tbr 5-bromo-2-deoxyuridine （BrdU, an indicator for cell proliferation） and doublecortin （DCX, an immature neuronal marker） and double immunofluorescence staining for BrdU and neuronal nuclear antigen （NeuN, a mature neuronal marker）. In addition, we examined expressional changes of brain-derived neurotrophic factor （BDNF） and its major receptor tropomyosin-related kinase B （TrkB） using Western blotting analysis. Results： Treatment with 200 mg/kg, not 100 mg/kg, significantly increased number of BrdU-immunoreactive （＋） and DCX＋ cells （48.0 ±3.1and 72.0 ± 3.8 cells/section, respectively） in the subgranular zone （SGZ） of the dentate gyrus （DG） and BrdU＊/NeuN＋ cells （17.0 ±1.5 cells/section） in the granule cell layer as well as in the SGZ. In addition, protein levels of BDNF and YrkB （about 232% and 244% of the vehicle-treated group, respectively） were significantly increased in the DG of the mice treated with 200 mg/kg ofG. littoralis extract. Conclusion： G. littoralis extract promots cell proliferation, neuroblast differentiation, and neuronal maturation in the hippocampal DG, and neurogenic effects might be closely related to increases ofBDN F and TrkB proteins by G. littoralis extract treatment.

关键词： Brain-Derived Neurotrophic Factor cellproliferation Glehnia littoralis Neuroblast Differentiation Tropomyosin-Related Kinase B

Composite bioabsorbable vascular stents via 3D bio-printing and electrospinning for treating stenotic vessels

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Journal of Southeast University(English Edition) 2015年第2期31卷 254-258页

作者：刘媛媛向科李瑜陈海萍胡庆夕上海大学快速制造工程中心上海200444 上海大学上海市智能制造及机器人重点实验室上海200072

A new type of vascular stent is designed for treating stenotic vessels. Aiming at overcoming the shortcomings of existing equipment and technology for preparing a bioabsorbable vascular stent （BVS）, a new method which combines 3D bio-printing and electrospinning to prepare the composite bioabsorbable vascular stent （CBVS） is proposed. The inner layer of the CBVS can be obtained through 3D bio- printing using poly-p-dioxanone （PPDO）. The thin nanofiber film that serves as the outer layer can be built through electrospinning using mixtures of chitosan-PVA （poly （vinyl alcohol））. Tests of mechanical properties show that the stent prepared through 3D bio-printing combined with electrospinning is better than that prepared through 3D bio- printing alone. Cells cultivated on the CBVS adhere and proliferate better due to the natural, biological chitosan in the outer layer. The proposed complex process and method can provide a good basis for preparing a controllable drug-carrying vascular stent. Overall, the CBVS can be a good candidate for treating stenotic vessels.

关键词： 3D ( three-dimensional ) bio-printing bioabsorbable vascular stent (BVS) electrospinning cellproliferation composite forming

Clinical and biological characteristics of colorectal cancer with familial predisposition

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Journal of Medical Colleges of PLA(China) 2001年第2期16卷 90-93页

作者：吴保平张亚历周殿元高春芳赖卓胜 Institute for Digestive Diseases of PLA Nanfang Hospital First Military University Guangzhou 510515 China

Objective:To evaluatethemicrosatelliteinstability(MSI),expressionof mismatchrepair(MMR)gene(hMLH1,hMSH2)andproliferationkineticsincolorectalcancer(CRC)***:Forty-sixcasesof CRC were studiedusingsilverstainingpolymerasechainreaction-singlestrandconformation polymorphism(PCR-SSCP)technique,streptavidin-peroxidase(SP)immunohistochemicalmethodand ***:In CRCpatientswithfamilialpredisposition,theMSI-positiveratewas higherthanin sporadicCRC(P<0.05).FamilialpredispositionandpositiveMSIwerestronglyrelatedto earlyageof canceronset,theproclivity for proximalcoloniccancer,poordifferentiatedandextracolorectalnmalignancies(P<0.01,P<0.05).Theincidence of negativeexpressionof hMLH1intumortissueandhMLH1,hMSH2innormalcolorectaltissueswassignificantly higher(P<0.05).Thenegativeexpressionof hMLH1togetherwithhMSH2was relatedwithpositiveMSI(P<0.05).InMSI-positiveCRCcells,theproliferationcellnucleusantigen(PCNA)expression,proliferationindexandS-phase cellsdecreasedobviously(P<0.01,P<0.05).Conclusion:In CRCwithfamilialpredisposition,MSI mightbe an *** hMLH1andhMSH2mutationincreasedintumorandnormaltissue,andtheproli-ferationactivityof theircancercellwaslower.

关键词： colorectalcancer familialpredisposition microsatelliteinstability mismatchrepairgene genemutation geneexpression cellproliferation

脂肪细胞因子Leptin对体外培养的RF/6A细胞自噬、VEGF表达及细胞增殖、迁移和管腔形成的影响

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医学临床研究 2022年第1期39卷 1-4页

作者：刘哲王旖李蓉巨伟杜斌马利张一凡杜军辉西安市第九医院交叉医学研究室陕西西安710054 西安医学院第一附属医院眼科陕西西安710077 西安市第九医院转化医学中心陕西西安710054 西安市第九医院眼视光中心陕西西安710054

【目的】探讨脂肪细胞因子Leptin对体外培养的RF/6A细胞自噬、血管内皮生长因子(Vascularendothelialgrowthfactor,VEGF)表达及细胞增殖、迁移和管腔形成的影响。【方法】以RF/6A细胞为研究对象,加入不同浓度Leptin培养24h后,WesternBlo... 详细信息

【目的】探讨脂肪细胞因子Leptin对体外培养的RF/6A细胞自噬、血管内皮生长因子(Vascularendothelialgrowthfactor,VEGF)表达及细胞增殖、迁移和管腔形成的影响。【方法】以RF/6A细胞为研究对象,加入不同浓度Leptin培养24h后,WesternBlotting检测蛋白微管相关蛋白1轻链3(lightchain3,LC3)、Beclin-1、p62及VEGF表达水平。Leptin联合自噬抑制剂3-甲基腺嘌呤(3-MA)、氯喹(CQ)作用RF/6A细胞后,用CCK8法检测细胞增殖、Transwell法检测细胞迁移、基质胶(Matrigel)法检测细胞管腔形成。【结果】与Control组比较,不同浓度Leptin均能上调LC3、Beclin-1、VEGF表达,抑制p62表达(P<0.05);与100ng/mLLeptin组比较,加入3-MA、CQ后均能抑制RF/6A细胞增殖,减少迁移细胞数和细胞管腔形成数(P<0.05)。【结论】Leptin能够激活RF/6A细胞自噬,促进VEGF表达、RF/RA细胞增殖、迁移和细胞管腔形成。

关键词：脂肪因子类细胞培养技术/方法自噬血管内皮生长因子A cellproliferation