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Acta Oceanologica Sinica 2000年第2期19卷 97-110页

作者： Sun Jun1 Liu Dengyan1 Qian Shuben1 (Environmental Ecology Department, Marine Life Sciences College of Ocean University of Qingdao, Qingdao 266003, China. ) Environmental Ecology Department Marine Life Sciences College Ocean University of Qingdao Qingdao 266003 China

Based on the data of the Jiaozhou Bay Ecosystem Dynamic Research, cell volume and sur face area of 87 common phytoplankton species in China sea waters were calculated with assignment of the similar geometric form. The cell plasma volume, live weight, Carbon content and nitrogen content were also calculated with the methods of Mullin et al. (1966), Strathmann (1967), Eppley et al. (1970), and Taguchi (1976). After comparing these methods, we chase the method of Eppley et al. (1970) as the hot method for calculating phytoplankton carbon content in China sea waters.

关键词： Phytoplankton cell volume plasma volume biomass carbon content nitrogen content

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How plants sense and respond to osmotic stress

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Journal of Integrative Plant Biology 2024年第3期66卷 394-423页

作者： Bo Yu Dai-Yin Chao Yang Zhao Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesThe Chinese Academy of SciencesShanghai 200032China Key Laboratory of Plant Carbon Capture The Chinese Academy of SciencesShanghai 200032China National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyThe Chinese Academy of SciencesShanghai 200032China University of Chinese Academy of Sciences Beijing 100049China

Drought is one of the most serious abiotic stresses to land *** sense and respond to drought stress to survive under water *** have studied how plants sense drought stress,or osmotic stress caused by drought,ever since Charles Darwin,and gradually obtained clues about osmotic stress sensing and signaling in *** stress is a physical stimulus that triggers many physiological changes at the cellular level,including changes in turgor,cell wall stiffness and integrity,membrane tension,and cell fluid volume,and plants may sense some of these stimuli and trigger downstream *** this review,we emphasized water potential and movements in organisms,compared putative signal inputs in cell wall-containing and cell wall-free organisms,prospected how plants sense changes in turgor,membrane tension,and cell fluid volume under osmotic stress according to advances in plants,animals,yeasts,and bacteria,summarized multilevel biochemical and physiological signal outputs,such as plasma membrane nanodomain formation,membrane water permeability,root hydrotropism,root halotropism,Casparian strip and suberin lamellae,and finally proposed a hypothesis that osmotic stress responses are likely to be a cocktail of signaling mediated by multiple *** also discussed the core scientific questions,provided perspective about the future directions in this field,and highlighted the importance of robust and smart root systems and efficient source-sink allocations for generating future high-yield stress-resistant crops and plants.

关键词： cell volume drought membrane tension osmotic stress turgor

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Measuring the size and growth of single cells

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Biophysics Reports 2022年第3期8卷 150-157页

作者： An Gong Mingwei Min Guangzhou Laboratory Guangzhou Institutes of Biomedicine and Health Chinese Academy of Sciences

The size and growth of a cell can be described by three related physical parameters: volume, density and mass. All the three are coupled to numerous biochemical reactions and biophysical properties of a cell. It is therefore not surprising that cell size and growth pattern are tightly regulated across all kingdoms of life. Indeed, deregulation of cell size and growth has been found to be associated with ***, how cells regulate their size and how cell size connects to cell function remain poorly understood,partly due to the difficulties to precisely measure the size and growth of single cells. In this review, we summarize methods of measuring cell volume, density, and mass, and discuss how the new technologies may advance our understanding of cell size control.

关键词： cell volume cell mass cell size cell density

Microstructural characterization and hydrogenation performance of ZrxV5Fe(x=3-9) alloys

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Progress in Natural Science:Materials International 2020年第2期30卷 229-238页

作者： Haiyan Leng Pan Yan Xingbo Han Wei Liu Qihang Liu Qian Li State Key Laboratory of Advanced Special Steels & Shanghai Key Laboratory of Advanced Ferrometallurgy & School of Materials Science and Engineering Shanghai University Shanghai Institute of Applied Physics Chinese Academy of Sciences

ZrxV5Fe(x=3,5,7,8,9) alloys were designed to investigate the influence of Zr addition on hydrogenation *** alloys were prepared by arc melting and then annealed at 1273 K for 168 *** results showed that the alloys were composed of α-Zr and C15-ZrV2 *** cell volume of C15-ZrV2phase firstly increased and then decreased as the content of Zr increased,while the reversed trend was found for the cell volume of α-Zr phase,which was related to the stoichiometric ratio of elements.α-Zr phase distributed in C15-ZrV2 phase matrix in ZrxV5Fe(x=5,7,8,9) alloys,among which Zr7 V5 Fe alloy showed the best *** PCT curves of the alloys under 623 K,673 K and 723 K showed that the hydrogen absorption plateau pressure of the phases in different alloys decreased gradually with the increasing content of *** C15-ZrV2 phase in Zr7 V5 Fe alloy had the lowest hydrogen absorption plateau pressure at room temperature,which was consistent with the change tendency of the corresponding cell ***,the kinetic curves of hydrogen absorption at 623 K revealed that Zr7 V5 Fe alloy with the smallest average particle size and the largest phase boundary area showed the fastest hydrogen absorption *** with other four alloys(including St707 alloy),Zr7V5Fe alloy is more suitable for the use of getter.

关键词： Non-evaporative getter Hydriding and dehydriding properties cell volume Hydrogen absorption dynamics Plateau pressure

Involvement of regulatory volume decrease in the migration of nasopharyn-geal carcinoma cells

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cell Research 2005年第5期15卷 371-378页

作者： Tim JACOB Cardiff School of Biosciences Cardiff University Biomedical Sciences Building Museum Avenue Cardiff CF10 3US UK

The transwell chamber migration assay and CCD digital camera imaging techniques were used to investigate the relationship between regulatory volume decrease (RVD) and cell migration in nasopharyngeal carcinoma cells (CNE-2Z cells). Both migrated and non-migrated CNE-2Z cells, when swollen by 47% hypotonic solution, exhibited RVD which was inhibited by extracellular application of chloride channel blockers adenosine 5’-triphosphate (ATP), 5-nitro-2-(3- phenylpropylamino) benzoic acid (NPPB) and tamoxifen. However, RVD rate in migrated CNE-2Z cells was bigger than that of non-migrated cells and the sensitivity of migrated cells to NPPB and tamoxifen was higher than that of non- migrated cells. ATP, NPPB and tamoxifen also inhibited migration of CNE-2Z cells. The inhibition of migration was positively correlated to the blockage of RVD, with a correlation coefficient (r) = 0.99, suggesting a functional relation- ship between RVD and cell migration. We conclude that RVD is involved in cell migration and RVD may play an important role in migratory process in CNE-2Z cells.

关键词： tumor cells chloride channel blockers cell volume cell migration transwell chamber.