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Translational Neuroscience and Clinics 2015年第2期1卷 125-127页

作者： Dario Siniscalco Nataliia Sych Department of Experimental Medicine Second University of Naples Clinical Department Cell Therapy Center Em Cell

Several debates have been raised over the use of stem cells in the field of regenerative medicine. Indeed, stem cell transplantation must follow strict rules. However, to date,many differences exist among different Western and Eastern European countries. This brief review summarizes the state‐of‐the‐art stem cell regulation in Italy, European Union, and Ukraine.

关键词： stem cells regulation cell transplantation clinical trial

Olfactory ensheathing cell transplantation for treatment of spinal cord injury An 18-year bibliometric analysis

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Olfactory ensheathing cell transplantation for treatment of ...

Safety evaluation in a study of human mesenchymal stem cell transplantation in the non-human primates

2012第二届国际神经再生高峰论坛

作者： Zikuan Leng Kai Cao Dong Wang Haopeng Li Xijing He Second Department of Orthopedics Second Affiliated Hospital Medical School of Xi’an Jiaotong University

Currently, curing spinal cord injury （SCI） is one of the most difficult problems worldwide. Olfactory ensheathing cell transplantation （OECT） is a putative new treatment. This bibliometric analysis was based on the Science Citation Index and conducted to demonstrate global research on OECT for SCI. Searches of the Institute of Scientific Information Web of Science used the Key words "olfactory ensheathing cells", "OECs", "olfactory ensheathing glia", "OEG", "olfactory ensheathing glia cells" or "OEGs" and "spinal cord injury", "SCI", "spinal injury" or "spinal transaction" limiting publication from 1985 to May 2012. Analyses were performed using analysis functions from the Web of Science data and statistics in Microsoft Excel. All selected literature was evaluated in the following aspects: Publication Year, Document Type, Language, Authors, Institutions, Times Cited, Web of Science Category, Source Title, Country/Territory and Funding Agencies. The earliest literature record was April 1995, and 414 articles addressing OECT for SCI were added to the data library in the past 18 years with an annually increasing trend. Of 415 records, 405 were in English. There were 259 articles ranked by document type, among which 10 articles are regarded as classic references. Ten core source titles published 143 articles （34.5% of 415）. The journal Experimental Neurology published the most literature （32 records） followed by Glia. The United States published the most articles, followed by China. In addition, Yale University was the most productive institution in the OECT for SCI field. Experimental Neurology published the most OECT literature in America, while Neural Regeneration Research published the most in China. This analysis demonstrates the current situation and trends of OECT for SCI research. Furthermore, the analysis could partly offer direction for international cooperation and teamwork on OECT for SCI, to obtain a more effective treatment for SCI.

关键词： olfactory ensheathing cells spinal cord injury cell transplantation spinal transection Science Citation Index bibliometric analysis

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Intraspinal transplantation of motoneuron-like cell combined with delivery of polymer-based glial cell line-derived neurotrophic factor for repair of spinal cord contusion injury

2011中华医学会神经外科学学术会议

作者： Ming Feng1 Xinjie Bao1 Yan Li1 Junji Wei1 Guilin Li1 Wenbin Ma1 Renzhi Wang1 1.Peking Union Medical College Hospital 北京协和医院

Objective The efficacy of stem cell transplantation in promoting the recovery of patients with neurological diseases, such as stroke, has been reported by several researchers. However, the safety of intracerebral tran... 详细信息

关键词： bone marrow mesenchymal stem cell cell transplantation safety pathology Macaca fascicularis

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Neural Regeneration Research 2014年第10期9卷 1003-1013页

作者： Alireza Abdanipour Taki Tiraihi Taher Taheri Shefa Neuroscience Research Center at Khatam Al-Anbia Hospital

To evaluate the effects of glial cell line-derived neurotrophic factor transplantation combined with adipose-derived stem cells-transdifferentiated motoneuron delivery on spinal cord con-tusion injury, we developed rat models of spinal cord contusion injury, 7 days later, injected adipose-derived stem cells-transdifferentiated motoneurons into the epicenter, rostral and caudal regions of the impact site and simultaneously transplanted glial cell line-derived neuro-trophic factor-gelfoam complex into the myelin sheath. Motoneuron-like cell transplantation combined with glial cell line-derived neurotrophic factor delivery reduced cavity formations and increased cell density in the transplantation site. The combined therapy exhibited superior promoting effects on recovery of motor function to transplantation of glial cell line-derived neurotrophic factor, adipose-derived stem cells or motoneurons alone. These ifndings suggest that motoneuron-like cell transplantation combined with glial cell line-derived neurotrophic factor delivery holds a great promise for repair of spinal cord injury.

关键词： nerve regeneration spinal cord injury adipose-derived stem cells glial cell line-derived neurotrophic factor motoneurons cell transplantation neurotrophic factor spinal cord contusion injury neural regeneration

Regenerative medicine strategies for chronic complete spinal cord injury

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Neural Regeneration Research 2024年第4期19卷 818-824页

作者： Shogo Hashimoto Narihito Nagoshi Masaya Nakamura Hideyuki Okano Department of Orthopedic Surgery Keio University School of Medicine Department of Physiology Keio University School of Medicine

Spinal cord injury is a condition in which the parenchyma of the spinal cord is damaged by trauma or various *** rapid progress has been made in regenerative medicine for spinal cord injury that was previously untreatable,most research in this field has focused on the early phase of incomplete ***,the majority of patients have chronic severe injuries;therefore,treatments for these situations are of fundamental *** reason why the treatment of complete spinal cord injury has not been studied is that,unlike in the early stage of incomplete spinal cord injury,there are various inhibitors of neural ***,we assumed that it is difficult to address all conditions with a single treatment in chronic complete spinal cord injury and that a combination of several treatments is essential to target severe ***,we established a combination therapy of cell transplantation and drug-releasing scaffolds,which contributes to functional recovery after chronic complete transection spinal cord injury,but we found that functional recovery was limited and still needs further ***,for the further development of the treatment of chronic complete spinal cord injury,we review the necessary approaches to the different pathologies based on our findings and the many studies that have been accumulated to date and discuss,with reference to the literature,which combination of treatments is most effective in achieving functional recovery.

关键词： cell transplantation chronic phase complete transection regenerative medicine spinal cord injury

cell replacement with stem cell-derived retinal ganglion cells from different protocols

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Neural Regeneration Research 2024年第4期19卷 807-810页

作者： Ziming Luo Kun-Che Chang Spencer Center for Vision Research Byers Eye InstituteStanford University School of Medicine Department of Ophthalmology Louis J.Fox Center for Vision RestorationUniversity of Pittsburgh School of Medicine Department of Neurobiology Center of NeuroscienceUniversity of Pittsburgh School of Medicine

Glaucoma,characterized by a degenerative loss of retinal ganglion cells,is the second leading cause of blindness *** is currently no cure for vision loss in glaucoma because retinal ganglion cells do not regenerate and are not replaced after *** stem cell-derived retinal ganglion cell transplant is a potential therapeutic strategy for retinal ganglion cell degenerative *** this review,we first discuss a 2D protocol for retinal ganglion cell differentiation from human stem cell culture,including a rapid protocol that can generate retinal ganglion cells in less than two weeks and focus on their transplantation ***,we discuss using 3D retinal organoids for retinal ganglion cell transplantation,comparing cell suspensions and *** review provides insight into current knowledge on human stem cell-derived retinal ganglion cell differentiation and transplantation,with an impact on the field of regenerative medicine and especially retinal ganglion cell degenerative diseases such as glaucoma and other optic neuropathies.

关键词： cell clumps cell suspension cell transplantation differentiation direct-induced protocol glaucoma optic neuropathy regenerative medicine retinal ganglion cell retinal organoids stem cells

transplantation of human induced pluripotent stem cell derived keratinocytes accelerates deep second-degree burn wound healing

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World Journal of Stem cells 2023年第7期15卷 713-733页

作者： Li-Jun Wu Wei Lin Jian-Jiang Liu Wei-Xin Chen Wen-Jun He Yuan Shi Xiao Liu Ke Li Department of Plastic and Aesthetic Surgery The Second Affiliated Hospital of Soochow UniversitySuzhou 215004Jiangsu ProvinceChina Department of Burn and Plastic Surgery The First Affiliated Hospital of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina

BACKGROUND Current evidence shows that human induced pluripotent stem cells(hiPSCs)can effectively differentiate into keratinocytes(KCs),but its effect on skin burn healing has not been *** To observe the effects of hiPSCs-derived KCs transplantation on skin burn healing in mice and to preliminarily reveal the underlying *** An analysis of differentially expressed genes in burn wounds based on GEO datasets GSE140926,and GSE27186 was established.A differentiation medium containing retinoic acid and bone morphogenetic protein 4 was applied to induce hiPSCs to differentiate into *** expression of KCs marker proteins was detected using immunofluorescence staining.A model of a C57BL/6 mouse with deep cutaneous second-degree burn was created,and then phosphate buffered saline(PBS),hiPSCs-KCs,or hiPSCs-KCs with knockdown of COL7A1 were injected around the wound *** wound healing,re-epithelialization,engraftment of hiPSCs-KCs into wounds,proinflammatory factor level,and the NF-κB pathway proteins were assessed by hematoxylin-eosin staining,carboxifluorescein diacetate succinimidyl ester(CFSE)fluorescence staining,enzyme linked immunosorbent assay,and Western blotting on days 3,7,and 14 after the injection,***,the effects of COL7A1 knockdown on the proliferation and migration of hiPSCs-KCs were confirmed by immunohistochemistry,EdU,Transwell,and damage repair *** HiPSCs-KCs could express the hallmark proteins of ***7A1 was down-regulated in burn wound tissues and highly expressed in *** of hiPSCs-KCs into mice with burn wounds resulted in a significant decrease in wound area,an increase in wound re-epithelialization,a decrease in proinflammatory factors content,and an inhibition of NF-κB pathway activation compared to the PBS *** in vitro assay showed that COL7A1 knockdown could rescue the inhibition of hiPSCs-KCs proliferation and migration,providing further evidence that COL7A

关键词： Induced pluripotent stem cell Keratinocytes cell transplantation Burn wound healing COL7A1

Are human dental papilla-derived stem cell and human brain-derived neural stem cell transplantations suitable for treatment of Parkinson’s disease?

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Neural Regeneration Research 2013年第13期8卷 1190-1200页

作者： Hyung Ho Yoon Joongkee Min Nari Shin Yong Hwan Kim Jin-Mo Kim Yu-Shik Hwang Jun-Kyo Francis Suh Onyou Hwang Sang Ryong Jeon Department of Neurological Surgery Asan Medical Center University of Ulsan College of Medicine Department of Biochemistry and Molecular Biology University of Ulsan College of Medicine Department of Computer Science and Engineering University of Notre Dame Notre Dame Center for Bionics of Korea Institute of Science and Technology Department of Maxillofacial Biomedical Engineering Institute of Oral Biology School of Dentistry Kyung Hee University

transplantation of neural stem cells has been reported as a possible approach for replacing impaired dopaminergic neurons. In this study, we tested the efficacy of early-stage human dental papilla-derived stem cells and human brain-derived neural stem cells in rat models of 6-hydroxydopamine-induced Parkinson＇s disease. Rats received a unilateral injection of 6-hydroxydopamine into right medial forebrain bundle, followed 3 weeks later by injections of PBS, early-stage human dental papilla-derived stem cells, or human brain-derived neural stem cells into the ipsilateral striatum. All of the rats in the human dental papilla-derived stem cell group died from tumor formation at around 2 weeks following cell transplantation. Postmortem examinations revealed homogeneous malignant tumors in the striatum of the human dental papilla-derived stem cell group. Stepping tests revealed that human brain-derived neural stem cell transplantation did not improve motor dysfunction. In apomorphine-induced rotation tests, neither the human brain-derived neural stem cell group nor the control groups （PBS injection） demonstrated significant changes. Glucose metabolism in the lesioned side of striatum was reduced by human brain-derived neural stem cell transplantation. [18F]-FP-CIT PET scans in the striatum did not demonstrate a significant increase in the human brain-derived neural stem cell group. Tyrosine hydroxylase （dopaminergic neuronal marker） staining and G protein-activated inward rectifier potassium channel 2 （A9 dopaminergic neuronal marker） were positive in the lesioned side of striatum in the human brain-derived neural stem cell group. The use of early-stage human dental papilla-derived stern cells confirmed its tendency to form tumors. Human brain-derived neural stem cells could be partially differentiated into dopaminergic neurons, but they did not secrete dopamine.

关键词： neural regeneration stern cells cell transplantation glucose metabolism human brain-derivedneural stem cells human dental papilla-derived stem cells Parkinson's disease positron emissiontomography grants-supported paper neuroregeneration

Survival and differentiation of transplanted neural stem cells in mice brain with MPTP-induced Parkinson disease

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Acta Pharmacologica Sinica 2003年第12期24卷 18-24页

作者： LI Xue-Kun, GUO An-Chen, ZUO Ping-Ping Department of Pharmacology, School of Basic Medicine, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100005, China Department of Pharmacology School of Basic Medicine Peking Union Medical College & Chinese Academy of Medical Sciences 北京 100005

AIM: To determine survival and differentiation of cultured neural stem cells (NSCs) into viable and functional neurons upon transplantation into mice brain of MPTP-induced Parkinson disease (PD). METHODS: Mouse model of PD was established with two subcutaneous (sc) injections of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 40 mg/kg) twice, 16h apart. NSCs isolated from rat embryo midbrain were cultured in clonal density. After labeled with 5-bromo-2’-deoxyuridine (BrdU), the NSCs were transplanted into the uni-or bi-lateral striatum of PD mouse. Tyrosine hydroxylase (TH) immunofluorescence was used to evaluate the toxicity of MPTP on the neural cells in the substantia nigra. Immunohistology and laser confocal microscope were used to detect the survival and differentiation of transplanted NSCs. RESULTS: The cultured NSCs generated neurospheres and differentiated into neuron and astrocyte. It indicated that the cultured NSCs were multipotent and self-renewal in vitro. TH-positive neural cells were

关键词： neural stem cells cell transplantation Parkinson disease cell survival cell differentiation

A Preliminary Study of the Therapeutic Role of Human Early Fetal Aorta-derived Endothelial Progenitor cells in Inhibiting Carotid Artery Neointimal Hyperplasia

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Chinese Medical Journal 2015年第24期128卷 3357-3362页

作者： Rong-Wei Xu Wen-Jian Zhang Jian-Bin Zhang Jian-Yan Wen Meng Wang Hong-Lin Liu Lin Pan Chang-An Yu Jin-Ning Lou Peng Liu Department of Cardiovascular Surgery China-Japan Friendship Hospital Beijing 100029 China Graduate School of Peking Union Medical College Chinese Academy of Medical Sciences Beijing 100073 China Institute of Clinical Medical Sciences China-Japan Friendship Hospital Beijing 100029 China

Background： Endothelial cell damage is an important pathophysiological step of restenosis after angioplasty and stenting. cell transplantation has great therapeutic potential for endothelial recovery. We investigated the effect of transplanting endothelial progenitor cells （EPCs） derived from human early fetal aortas in rat injured arteries. Methods： The carotid arterial endothelium of Sprague-Dawley rats was damaged by dilatation with a 1.5 F balloon catheter, and then EPCs derived from human early fetal aortas （〈14 weeks） were injected into the lumen of the injured artery in transplanted rats, with an equal volume of normal saline injected into control rats. Rats were sacrificed at 2 and 4 weeks after treatment and transplanted cells were identified by immunohistochemical staining with anti-human CD31 and anti-human mitochondria antibodies. Arterial cross-sections were analyzed by pathology, immunohistochemistry, and morphometry. Results： Green fluorescence-labeled EPCs could be seen in the endovascular surface of balloon-injured vessels after transplantation. The intimal area and intimal/medial area ratio were significantly smaller in the transplanted group than in the control （P 〈 0.05） and the residual lumen area was larger （P 〈 0.05）. After EPC transplantation, a complete vascular endothelial layer was formed, which was positive for human yon Willebrand factor after immunohistochemical staining, and immunohistochemical staining revealed many CD31- and mitochondria-positive cells in the re-endothelialized endothelium with EPC transplantation but not control treatment. Conclusion： EPCs derived from human early fetal aorta were successfully transplanted into injured vessels and might inhibit neointimal hyperplasia after vascular injury.

关键词： Carotid Artery Injury cell transplantation Endothelial Progenitor cell Human Fetal Aorta Neointima