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Effect of Dietary Supplementation with Hydrolyzed Wheat Gluten on Growth Performance, cell immunity and Serum Biochemical Indices of Weaned Piglets (Sus scrofa)

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Agricultural Sciences in China 2011年第6期10卷 938-945页

作者： WANG Xiu-qi FENG You SHU Gang JIANG Qing-yan YANG Jing-pei ZHANG Zi-feng College of Animal Science South China Agricultural University Guangzhou 510642 P.R. China Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding Guangzhou 510642 P.R.China Zhengzhou Newwill Nutrition Technology Co. Ltd. Zhengzhou 450000 P.R. China

To investigate the effects of dietary supplementation with hydrolyzed wheat gluten （HWG） on growth performance, cell immunity and serum biochemical indices of weaned piglets, 160 crossed （Large White×andrace） and weaned piglets were randomly divided into 4 treatments with 4 replicates of 10 piglets each. The piglets in each treatment were fed an experimental diet containing either 0 g kg-1 HWG （control group）, 30 g kg-1 HWG （3% HWG group）, 50 g kg-1 HWG （5% HWG group）, or 2.5 g kg-1 glycyl-L-glutamine （0.25% Gly-Gln group）. The results showed that the diarrhea rates in 3% HWG and 5% HWG groups were significantly lower than in control group from d 1 to 14 （P〈0.05）, while the average daily gain （ADG） in each of two groups was increased （P〉0.05）. When compared with control group, dietary supplementation with 3% HWG increased the ratio of CD4＋：CD8＋ and the ratio of serum albumin and globulin concentrations （A：G） on d 14 and 28, as well as the proliferation of T- and B-lymphocytes （P〉0.05） on d 28. In addition, on d 14 and 28, the A：G ratio in 5% HWG group was significantly higher than in control group （P〈0.05）, while the ratio of CD4＋：CD8＋ increased slightly （P〉0.05）. Interestingly, 0.25% Gly-Gln group had higher proportion of CD3＋（P〉0.05） and CD4＋（P〈0.05） on d 14 than control group, but growth performances of 0.25% Gly-Gln group were negatively affected at all experiment stages. These results suggested that HWG might improve the growth performance of piglets by strengthening cell immunity and decreasing the occurrence of diarrhea during the prophase after weaning.

关键词： hydrolyzed wheat gluten weaned piglets growth performance cell immunity serum biochemical indices

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Effect of Astragalus Polysaccharide on the cell-mediated immunity of Traumatic Stress Mice

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Journal of Microbiology and Immunology 2004年第2期2卷 126-130页

作者：曾广仙熊金蓉刘俊英廖奕华代丽红李杏娟沈关心 Department of Pathology No.457 Hospital of PLA Wuhan 430012 China Department of Immunology of Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030 China

To investigate the changes of immune functions and the effects of Astragalus polysaccharide (ASP) on the cell-mediated immunity of the traumatic stress model of mouse by amputation, 50 mice were randomly divided into 5 groups for study, in which the group A and B served as the normal control (by injecton of 0.5 ml of saline intra-peritoneally daily), and as the stress control (by intra-peritoneal injecton of 0.5 ml of normal saline into mice after amputation) respectively, to the group C, D and E of mice, 1000 mg/kg (high dose), 300 mg/kg (median dose) and 250 mg/kg (low dose). The CD4 + and CD8 + T cells as well as the expression of the c-fos protein were determined by immunohistochemical techniques, and the expressions of NF-κB mRNA and IL-10 mRNA were assayed by hybridization in situ . The experimental results showed that in comparison with the normal control group of mice (group A), the expression levels of NF-κB mRNA, IL-10 mRNA and the c-fos protein in the tissues of thymus and spleen in the stress controls were significantly elevated and the CD4 + T cells and CD4/CD8 ratio were decreased. However, in comparison with the stress control of mice (group B), the expressions of NF-κB mRNA and IL-10 mRNA were inhibited by ASP, and the CD4 + T cells and CD4/CD8 ratio were increased in groups C, D and E, but the level of c-fos protein was decreased. There was no significant difference in these parameters among group C, D and E. It is concluded that the functions of cell-mediated immunity of mice were disturbed under the stress condition of the traumatic injuries after amputation. And the immune functions can be effectively restored by the use of Astragalus polysaccharide.

关键词： Trauma and injury cell immunity Astragalus polysaccharide IL-10 NF-κB T lymphocyte subsets c-fosImmunohistochemistry Hybridization in situ

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Anti-tumor Immune Response Mediated by Newcastle Disease Virus HN Gene

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Chemical Research in Chinese Universities 2011年第2期27卷 282-286页

作者： PENG Li-ping LI Xiao SUN Li-li WEN Zhong-mei LIU Yan GAO Peng HUANG Hai-yan PIAO Bing-guo JIN Jing J1N Ning-yi Department of Respiratory Medicine the First Hospital of Jilin University Changchun 130021 P R. China Genetic Engineering Laboratory of PLA the Eleventh Institute of Academy of Military Medical Sciences of PLA Changchun 130062 P R. China Head and Neck Surgery the Tumor Hospital of Jilin Province Changchun 130012 P R. China Department of Gastroenterology First Hospital of Jilin University Changchun 130021 P R. China Department of Hematology and Oncology People＇s Hospital of Jilin Province Changchun 130021 P R. China College of Pharmacy Changchun University of Chinese Medicine Changchun 130117 P R. China

Hemagglutinin-neuramidinase（HN） is one of the most important surface structure proteins of the Newcastle disease virus（NDV）. HN not only mediates receptor recognition but also possesses neuraminidase（NA） activity, which gives it the ability to cleave a component of those receptors, NAcneu. Previous studies have demonstrated that HN has interesting anti-neoplastic and immune-stimulating properties in mammalian species, including humans. To explore the application of the HN gene in cancer gene therapy, we constructed a Lewis lung carcinoma（LLC） solid tumor model using C57BL/6 mice. Mice were injected intratumorally with the recombinant adenovirus expressing HN gene（Ad-HN）, and the effect of HN was explored by natural killer cell activity assay, cytotoxic lymphocyte activity assay, T cell subtype evaluation, and Th1/Th2 cytokines analysis. The results demonstrate that HN not only can elicit clonal expansion of both CD4＋ and CD8＋ T cell populations and cytotoxic T lymphocyte（CTL） and killer cell response, but also skews the immune response toward Th1. Thus, vaccination with Ad-HN may be a potential strategy for cancer gene therapy.

关键词： HN gene Recombined adenovirus Cytokine cell immunity Anti-tumor activity

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婴儿手术后细胞免疫的变化

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中华小儿外科杂志 1984年第3期5卷 134-135页

作者：郑玉衡佘亚雄丁其珍钱龙华上海市儿科研究所外科研究室上海第二医学院附属新华医院儿外科上海市儿科研究所免疫实验室

Institute Possible changes in the response of lymphocytes to phytohemagglutinin(PHA)were investigated on 20 infants with various surgical *** result of incorporation of 3 H-thymidine following PHA stimulation Indicates a depression in cell immune response within 24～48 hours after operation in these infants and then it gradually *** observed was that greater blood loss and anesthesia with ether during operation could obviously affect immune *** is suggested that proper anesthesia procedures and agents and adequate transfusion could reduce the possible damage to the immune ***;antibiotics are necessary to prevent postoperative infections.

关键词：婴儿手术后细胞免疫 Operation cell immunity cell immune response operation 胸腺嘧啶核苷免疫功能抑制淋巴细胞转化 blood loss PHA 术后感染麻醉下 changes 掺入法 during agents 资料预防

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Analysis of Epstein-Barr viral DNA load, EBV-LMP2 specific cytotoxic T-lymphocytes and levels of CD4^＋CD25^＋ T ceils in patients with nasopharyngeal carcinomas positive for IgA antibody to EBV viral capsid antigen

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Chinese Medical Journal 2009年第10期122卷 1173-1178页

作者： MO Wu-ning TANG An-zhou ZHOU Ling HUANG Guang-wu WANG Zhan ZENG Yi Laboratory Department First Affiliated Hospital Guangxi Medical University Nanning Guangxi 530021 China Department of Otolaryngology & Head and Neck Surgery First Affiliated Hospital Guangxi Medical University Nanning Guangxi 530021 China State Key Laboratory for Infectious Disease Prevention and Control Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100052 China

Background Epstein-Barr virus （EBV） is a herpesvirus commonly associated with several malignant diseases including nasopharyngeal carcinoma （NPC）, which is a common cancer in Southeastern Asia. Previous studies showed that plasma levels of EBV-DNA might be a sensitive and reliable biomarker for the diagnosis, staging and evaluating of therapy for NPC. There are a few analyses of the levels of EBV-latent membrane protein 2 （LMP2）-specific cytotoxic T-lymphocytes （CTLs） in patients with NPC. This study was conducted to investigate the levels of EBV-LMP2-specific CTLs, EBV-DNA load and the level of CD4^＋CD25^＋T cells in such patients. Methods From February 2006 to April 2006, 62 patients with NPC, 40 healthy virus carriers positive for EBV viral capsid antigen （EBV-IgA-VCA） and 40 controls were enrolled in the study. We used a highly sensitive ELISPOT assay, real-time polymerase chain reaction （PCR） and flow cytometry to measure the EBV-LMP2-specific CTL response, the EBV DNA load and the level of CD4^＋CD25^＋T cells, respectively. Results The EBV-LMP2-specific CTL responses of the samples from the control, healthy virus carriers and patients with NPC were significantly different from the LMP2 epitopes, with the control and healthy virus carrier samples displaying a stronger response in three cases. There were significant differences in EBV DNA load in serum between NPC and the healthy groups; patients with NPC at stages Ⅲ or Ⅳ had significantly higher viral loads compared with those at stages Ⅰ or Ⅱ. A significantly higher percentage of CD4^＋CD25^＋ T lymphocytes were detected in the patients, compared with healthy virus carriers and healthy controls. Moreover, patients with advanced stages of NPC （Ⅲ and Ⅳ） had significantly higher percentages than the patients with early stages （Ⅰ and Ⅱ）. Conclusions Patients with NPC are frequently unable to establish or maintain sufficient immunosurveillance to control proliferating B cells harboring EBV and to destroy the tumor

关键词： nasopharyngeal carcinoma cell immunity Epstein-Barr virus latent membrane protein 2 cytotoxic T lymphocyte

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Immunotoxicity of Ethephon in Mice

Immunotoxicity of Ethephon in Mice

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中国食品科学技术学会第五届年会暨第四届东西方食品业高层论坛

作者： Zhao Nan Zhao Wen Liu Yifeng Zhang Huiyan (College of Food Science,Hebei Agricultural University,Baoding 071000)

The aim of the study is to investigate the effects of ethephon on immunity in *** assay on plaque forming cell(PFC)of spleen,content of serum hemolysin,delayed hypersensitivity and macrophage function were carried *** aim is to study the toxicities on humeral immunity,cell immunity and non-special immunity of *** results showed that the relative weights of spleen,the macrophagocye index,the thickness of footput,the numbers of plaque forming cell and the serum HC50,in 0.462ml/kgbw and 0.231 ml/kgbw dosage level,were significantly lower than those in the control group(P＜0.05,P＜0.01).The results suggested that there are notable inhibitive effects of ethephon on immunity in mice.

关键词： ethephon immunotoxicity cell immunity humeral immunity non-special

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Morphological Changes of Hemocytes of Musca domestica Larva in vitro Infected by Escherichia coli

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Agricultural Science & Technology 2010年第3期11卷 115-117页

作者：晏容刘晖贺莉芳刘流遵义医学院寄生虫学教研室贵州遵义563003

[Objective] The research aimed to observe the effects of Escherichia coli infection on the morphology of hemocytes of the 3rd stage larva of Musca domestica in vitro and understand the hemocytes types that take part in the cell immunity of Musca domestica larval.[Method] The hemcytes of the 3rd stage larva of Musca domestica were cultured in vitro and the hemocyte morphology was observed about 2,4,6,8 h after culture in *** Escherichia coli were injected into the hemocytes of the 3rd stage larva of Musca domestica in vitro,the morphology changes of hemocytes were observed at different time after infection.[Result] The hemocytes of of the 3rd stage larva of Musca domestica was divided into five types about 2 h after *** hemocytes partly adherence was seen about 6 h after *** vacuolation and morpholysis was found in plasmatocytes after being infected by *** and a great quantity bacterium were gathered around granulocyte,but the morphology changes of hemocytes were not found in the prohemocyte,shprulocyte and oenocytoid.[Conclusion] The plasmatocyte and granulocyte were primary participants of the cell immunity of Musca domestica larval,but the prohemocyte,sphrulocyte and oenocytoid do not participate in the cell immune reactions.

关键词： Musca domestica larval Escherichia coli Hemocyte Morphology cell immunity

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