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In vitro morphogenic response of leaf sheath of Phyllostachys bambusoides

Journal of Forestry Research 2011年第2期22卷 209-215页

作者： Yurika H.Komatsu Katherine Derlene Batagin-Piotto Gilvano Ebling Brondani Antnio Natal Gonalves Marcílio de Almeida Graduate Program in Forest Resources Department of Forest Sciences"Luiz de Queiroz" College of AgricultureUniversity of So Paulo(ESALQ/USP)Avenida Pádua Dias11PiracicabaSo PauloBrazil Graduate Program in Biochemistry of Plants Department of Biological Sciences"Luiz de Queiroz" College of AgricultureUniversity of So Paulo(ESALQ/USP)Avenida Pádua Dias11PiracicabaSo Paulo

Nodal segments from secondary branches of saplings of Phyllostachys bambusoides were inoculated in MS medium to assess the in vitro morphogenic response of leaf sheath through the induction to callogenesis by Picloram（4-amino-3,5,6-trichloropicolinic acid） at different concentrations of carbohydrate under the same conditions with presence or absence of *** our experiment,secondary explants were kept in MS medium containing 8.0 mg·L-1 of Picloram for the callus *** were transferred in MS medium supplemented with sucrose,fructose and glucose（control,2%,4% and 6%）.Results show that Picloram induced the callogenesis in leaf *** secondary embryogenesis was formed in yellow-globular *** sucrose as carbohydrate source in the absence of light was more efficient to induce *** was more efficiency in the presence of *** induction and further embryogenesis evidenced the competence and determination of leaf sheath cells.

关键词： poaceae tissue culture callus induction cell competence determination

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Responses of the Host Plant Tissues to Gall Induction in Aspidosperma spruceanum Mü***.(Apocynaceae)

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American Journal of Plant Sciences 2011年第6期2卷 823-834页

作者： Anete Teixeira Formiga Geraldo Luiz Goncalves Soares Rosy Mary dos Santos Isaias Departamento de Botanica Campus da PampulhaInstituto de Ciencias BiologicasUniversidade Federal de Minas GeraisBelo HorizonteBrazil Departamento de Botanica Campus do ValeInstituto de BiocienciasUniversidade Federal do Rio Grande do SulPorto AlegreBrazil

The ontogenetic characterization of the leaf galls induced in the internervural region and in the second and third order veins of A. spruceanum Müell Arg. (Apocynaceae) aims to evaluate the distinct levels of cell reaction during the process of gall formation, and the relation between external gall morphology and the oviposition sites. The ground system had the most remarkable alterations, namely, the non differentiation of palisade parenchyma in both leaf sides, the hyperplasia of the spongy parenchyma and the neoformation of fibersclereids, a cell type not observed in non galled leaves. Changes of the feeding sites inside the larval chamber reveal distinct levels of cell competence to respond to the insects stimuli and explain the variations in the shape of the larval chamber.

关键词： Aspidosperma cell competence Gall Development Leaf Anatomy

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