检索结果-南通市图书馆

聚集体(英文) 2023年第2期4卷 300-308页

作者： Guo-Qiang Zhang Weiwei Feng Zhiyuan Gao Gao-Lan Zhang Xinying Wu Yating Xiao Xun Li Lei Zheng Dan Ding Jianshuang Guo Bo Situ Frontiers Science Center for cell Responses State Key Laboratory of Medicinal Chemical Biology Key Laboratory of Bioactive MaterialsMinistry of Educationand College of Life SciencesNankai UniversityTianjinChina Department of Laboratory Medicine Nanfang HospitalSouthern Medical UniversityGuangzhouChina Department of Pharmacology and Molecular Sciences Johns Hopkins University School of MedicineBaltimoreMarylandUSA School of Molecular Medicine Hangzhou Institute for Advanced StudyUCASHangzhouChina School of Chemical Engineering University of Chinese academy of scienceBeijingChina

Detection and imaging of α-L-fucosidase(AFU)is of great value to understand its roles in hepatocellular carcinoma(HCC)and tumor early diagnosis,but ideal assays are still lacking.Herein,a near-infrared(NIR)fluorescent biosensor(α-Fuc-DCM)was elaborately designed and synthesized for rapid and ratiometric detection of AFU activity in cells and HCC tumor mouse models.In the presence of AFU,this biosensor shows an enhancement in NIR emission in a ratiometric manner,which significantly improves the detection accuracy with the limit of detection as low as 4.8 mU/mL.Taking advantage of these merits,the activity of AFU in lysosomes could be visualized using ratiometric and NIR dual modality in living cells.Furthermore,its remarkable application for monitoring of endogenous AFU activity in HCC tumor-bearing mouse model is also demonstrated with bright fluorescence signal,which indicated that the biosensor could clearly monitor the liver tumor in the early stage.Importantly,the α-Fuc-DCM probe can be utilized to detect the AFU in serum from HCC patients.This strategy offers a promising biosensor system for early diagnosis of HCC and studying the roles of AFU in cancers.

关键词： cell and tumor imaging NIR fluorescent nanoprobe α-L-fucosidase

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