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Effects of cecropin-xj on growth and adherence of oral cariogenic bacteria in vitro

中华医学杂志(英文版) 2005年第2期000卷 69-74页

作者： HAO Yu qing, ZHOU Xue dong, XIAO Xiao rong, LU Jun jun, ZHANG Fu chun, HU Tao, WU Hong kun and CHEN Xin mei Key Laboratory for Oral Biomedical Engineering, Ministry of Education, West China of Stomatology College, Sichuan University, Chengdu 610041, China Key Laboratory of Molecular Biology, College of Life Science and Technology, Xinjiang University, Urumq, 830000, China Key Laboratory for Oral Biomedical Engineering Ministry of Education West China of Stomatology College Sichuan University Chengdu 610041 China Key Laboratory of Molecular Biology College of Life Science and Technology Xinjiang University Urumq 830000 China

Background cecropinxj belongs to cecropinB, which is the most potent antibacterial peptide found naturally The aim of this study was to investigate the effects of cecropinxj on growth and adherence of oral cariogenic bacteriaMethods Four oral cariogenic bacteria (Streptococcus mutans, Lactobacillus acidophilus, Actinomyces viscosus and Actinomyces naeslundii) were chosen for this experiment The minimal inhibitory concentrations (MICs) and reductive percent of bacterial growth were used to assay the antibacterial activity of cecropinxj Mammalian cytotoxicity of cecropinxj was tested with human periodontal membrane fibroblasts by tetrazolium (MTT) colorimetric assay The bacterial morphological changes induced by cecropinxj were examined on scanning electron microscope (SEM) The influence of cecropinxj on bacterial adhesion to salivacoated hydroxyapatite (SHA) was measured by scintillation countingResults The MICs of cecropinxj for inhibition of the growth of four bacteria ranged from 40 to 428 μmol/L with the highest susceptible to A naeslundii and the lowest susceptible to L acidophilus At pH 68, 55 and 82, 1/2 MIC of cecropinxj reduced the number of viable bacteria by 409%, 678% and 328% for S mutans and by 281%, 572% and 379% for L acidophilus The activities against Smutans and L acidophilus increased at pH 55 compared with pH 68 (P005, respectively), whereas almost no reduction counts were detected in the presence of 20% serum for both bacteria (P>005, respectively) Mammalian cytotoxicity of cecropinxj from 10 to 100 μmol/L exhibited no cytotoxicity against human periodontal membrane fibroblasts (P>005) bacterial morphological changes induced by MIC of cecropinxj examined on SEM showed cell s

关键词： cecropin-xj · cariogenic agents · anti bacterial agents · bacterial adhesion · durapatite

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