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Effects of catechins on litter size,reproductive performance and antioxidative status in gestating sows

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Animal Nutrition 2015年第4期1卷 271-275页

作者： Zhiyong Fan Yong Xiao Yonghui Chen Xin Wu Guanglei Zhang Qinhua Wang Chunyan Xie Engineering Research Center for Feed Safety and Efficient Utilization of Ministry of Education Institute of Animal Nutrition Hunan Agricultural University Institute of Subtropical Agriculture Chinese Academy of Sciences

This study was conducted to investigate the effects of catechins on reproductive performance, antioxidative capacity and immune function of gestating sows. A total of 60 cross-bred（Landrace × Large White） multiparious sows were blocked by body weight, parity and backfact and randomly allocated to 1 of 5 treatments: 0.100,200,300, or 400 mg/kg catechins. Dietary treatments were imposed from mating to d 40 of gestation of sows. At farrowing, litter total born, born alive, dead, and normal-（healthy piglets,>0.85 kg） and low-birth weight piglets（catechins at levels of 200 or 300 mg/kg led to improvements in litter born alive（P catechins at any supplemental levels all enhanced the serum SOD（P 0.05）. Sows received 200 mg catechin per kg diets showed a reduction（P catechins may be a potential antioxidant to increase the reproductive performance and antioxidative capacity of sows when it was added into diets during the early g

关键词： Antioxidative capacity catechins Gestating sows Litter size Reproductive performance

Qualitative analysis of catechins from green tea GMB-4 clone using HPLC and LC-MS/MS

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Asian Pacific Journal of Tropical Biomedicine 2015年第12期5卷 1046-1050页

作者： Erna Susanti Ciptati Retty Ratnawati Aulanni'am Achmad Rudijanto Doctoral Program of Biomedical Science Faculty of Medicine Brawijaya University Department of Chemistry Faculty of Science Bandung Institute of Technology Biomedical Science Faculty of Medicine Brawijaya University

Objective:To identify the bioactive compounds in catechins isolation and its components from green tea GMB-4 clone.Methods:Green tea GMB-4 clones were extracted with distilled water at 90C.Samples were eluted into the column with 10%ethanol.Subsequently,the column was eluted with95%ethanol and evaporated separately.Green tea extract was identified by thin layer chromatography.catechins were separated by the stationary phase in column chromatography using polyamide with 10%ethanol eluent and 95%ethanol.The results of isolations were analyzed by high performance liquid chromatographic(HPLC)and LCMS/MS.Analysis of catechins by HPLC was done by external standard.Results:Fraction from 10%ethanol showed that four major peaks at retention time of1.663,2.367,2.950 and 4.890,indicated the presence of four catechins components including catechin,epicatechins,gallocatechin and epigallocatechin.Whereas,fraction from 95%ethanol showed two main peaks at retention time of 5.167 and 9.82,which indicated the presence of epigallocatechin gallate(EGCG)and epicatechin gallate(ECG).EGCG(m/z 459),epigallocatechin(m/z 307),ECG(m/z 443),and epicatechin(m/z 291)were isolated and separated successfully using HPLC and LC-MS/MS.Conclusions:The HPLC and LC-MS/MS methods were successfully tuned for the qualitative analysis of green tea extract with EGCG and ECG.Four major catechins were separated and identified by LC-MS/MS,such as EGCG,epigallocatechin,ECG and epicatechin.The result of HPLC analysis showed that EGCG and ECG were main components from catechins isolation of green tea GMB-4 clone.

关键词： catechins Green tea GMB-4 clone LC-MS/MS HPLC

catechins Green Tea GMB4 Clone Increases mRNA of ABCA1 through LXR Signaling in Cultured Macrophage Exposed OX-LDL

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Food and Nutrition Sciences 2019年第9期10卷 1179-1190页

作者： Erna Susanti Retty Ratnawati Aulanni’am Aulanni’am Achmad Rudijanto Pharmacy and Food Analysis Academy “Putra Indonesia Malang” Malang Indonesia Doctoral Program of Biomedical Science Medical Faculty Brawijaya University Malang Indonesia

Inhibition of atherogenesis through inhibition of lipid metabolism has not been explored while other inhibitions through inflammation, endothelial dysfunction and free radicals have done. Inhibition of atherogenesis via inhibition of lipid metabolism can be done through the mechanism of Reverse Cholesterol Transport (RCT). Signaling pathways that play a role in this mechanism is LXR signaling. LXR activation by an LXR agonist led increasing cholesterol efflux. catechins based on bioinformatics study showed as a potent candidate LXR agonists that can be used as an inhibitor of atherogenesis. This study aims to prove that the administration catechins green tea Clone GMB4 can prevent atherosclerosis through increasing mechanism cholesterol efflux from macrophage by taking effect of ABCA1, ABCG1, SRB1 gene expression in cultured macrophages were exposed ox-LDL. Long-term goals of the outcome of the research are the use of catechins Green Tea Clones GMB4 as an inhibitor of atherogenesis so that it can be used as a complementary therapy for the treatment of atherosclerosis and cardiovascular diseases. The research is divided into 5 groups, namely the culture of macrophages without exposed ox-LDL, culture exposed ox-LDL and groups of catechins dose I, II, III. In vitro study showed that administration of catechins increases mRNA of ABCA1, whereas mRNA ABCG1 and SRB1 decreased at all three doses given. The result of protein profilling was identified a protein with a molecular weight of 70 kDa by SDS-PGE with silver staining.

关键词： Atherogenesis catechins ABCA1 ABCG1 Dan SRB1 Cultured Macrophage

Lipopolysaccharide-Deficient Mutants of <i>Salmonella enterica</i>Have Increased Sensitivity to catechins

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Advances in Microbiology 2013年第5期3卷 399-402页

作者： Miho Yoshii Akira Okamoto Michio Ohta Department of Bacteriology Nagoya University Graduate School of Medicine Nagoya Japan

Antimicrobial activity is one of the well-known biological characteristics of catechins, the main extract of green tea leaves. It is thought that catechins intercalate into the bacterial cell membrane and damage the lipid bilayer. However, the association between catechins and lipopolysaccharides, which consist of an O side chain, core oligosaccharide, and lipid A, has not been previously investigated. In this study, we evaluated the catechin sensitivity of Salmonella enterica mutants that lack the O side chain and have core oligosaccharides of different lengths. These rough mutants were more sensitive to catechins than a bacterial strain with intact lipopolysaccharide. We conclude that the O side chain and core oligosaccharide play an important role in protecting Gram-negative bacteria against the antimicrobial activity of catechins.

关键词： Antimicrobial Activity catechins Lipopolysaccharide Gram-Negative Bacteria

Effects of thermally modified green tea catechins on the oxidative and hydrolytic stability of butter

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Health 2009年第3期1卷 192-196页

作者： Magdalena Mika Agnieszka Wikiera Krzysztof Zyla Department of Food Biotechnology Faculty of Food TechnologyUniversity of Agriculture in KrakowKrakowPoland

Green tea catechins are classified as (-)-epi-forms (2R, 3R) or (-)-forms (2S, 3R) with respect to stereoisomerism. The (-)-forms (2S, 3R) in catechin preparations obtained from green tea amounts to approximately 10% of total catechins, whereas the other 90% are (-)-epiforms (2R, 3R). High temperature induces the conversion of (-)-epiforms (2R, 3R) to (-)-forms (2S, 3R). This study investigated the effect of catechin prepa-rations containing 10, 20 and 30% (-)-forms (2S, 3R) on the oxidative and hydrolytic stability of butter. For comparison, butter with δ-tocopherol and BHT and butter without stabilizer were analysed. Butter stability was examined under conditions of refrigeration (8 oC) and freezing (-22 oC) and at temperature of 50 oC and 100 oC. Catechin preparations were more efficient butter stabilizers than BHT and δ-tocopherol. Thermal modification of catechins that led to the genera-tion of 20% of (-) forms (2S, 3R) improved their antioxidative efficacy, but longer treatment lead- ing to the formation of 30% of (-) forms (2S, 3R) decreased their antioxidative activity. The hy-drolytic stability of butter, however, increased as the amount of (-) forms (2S, 3R) increased.

关键词： Oxidative Hydrolytic Stability Antioxidants catechins

Chemoprevention of obesity-related liver carcinogenesis by using pharmaceutical and nutraceutical agents

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World Journal of Gastroenterology 2016年第1期22卷 394-406页

作者： Hiroyasu Sakai Yohei Shirakami Masahito Shimizu Department of Gastroenterology/Internal Medicine Gifu University Graduate School of Medicine

Obesity and its related metabolic disorders are serious health problems worldwide,and lead to various healthrelated complications,including cancer.Among human cancers,hepatocellular carcinoma(HCC)is one of the most common malignancies affected by obesity.Therefore,obesity and its related disorders might be a key target for the prevention of HCC.Recently,new research indicates that the molecular abnormalities associated with obesity,including insulin resistance/hyperinsulinemia,chronic inflammation,adipokine imbalance,and oxidative stress,are possible molecular mechanisms underlying the pathogenesis of obesityrelated hepatocarcinogenesis.Green tea catechins and branched-chain amino acids,both of which are classified as nutraceutical agents,have been reported to prevent obesity-related HCC development by improving metabolic abnormalities.The administration of acyclic retinoid,a pharmaceutical agent,reduced the incidence of HCC in obese and diabetic mice,and was also associated with improvements in insulin resistance and chronic inflammation.In this article,we review the detailed molecular mechanisms that link obesity to the development of HCC in obese individuals.We also summarize recent evidence from experimental and clinical studies using either nutraceutical or pharmaceutical agents,and suggest that nutraceutical and pharmaceutical approaches targeting metabolic abnormalities might be a promising strategy to prevent the development of obesity-related HCC.

关键词： Hepatocellular carcinoma Obesity Greentea catechins Branched-chain amino acids Acyclicretinoid

LC-ESI-IT-MS/MS and MALDI-TOF Approach:Identification of Natural Polymers from Rhizophora mangle Barks and Determination of Their Analgesic and Anti-inflammatory Properties

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Natural Products and Bioprospecting 2019年第1期9卷 23-34页

作者： Leonardo Mendes de Souza Mesquita Caroline Fabri Bittencourt Rodrigues Claudia Quintino da Rocha Mayara Silveira Bianchim Clenilson Martins Rodrigues Vanda Maria de Oliveira Henrique Hessel Gaeta Mariana Novo Belchor Marcos Hikari Toyama Wagner Vilegas Laboratory of Bioprospection of Natural Products(LBPN) UNESP-Sao Paulo State University/Coastal Campus of Sao VicentePc¸a Infante Dom Henrique S/NSao VicenteSao Paulo CEP:11330-900Brazil Laborato´rio de Estudos Avanc¸ados em Fitomedicamentos(LEAF) UFMA-Federal University of MaranhaoAv.dos Portugueses1966-BacangaSao Luı´sMaranhao CEP:65080-805Brazil Embrapa Agroenergy Brazilian Agricultural Research CorporationW3 NortePqEBBrası´liaDF 70770-901Brazil

We recognize the chemical composition of the acetonic extract of Rhizophora mangle barks(AERM)using mass spectrometry analysis[liquid chromatography(LC)–ESI–IT-MS/MS and matrix-assisted laser desorption/ionization-time of flight-MS(MALDI-TOF)].Analgesic activity was evaluated by formalin and tail-flick experimental assays.Anti-inflammatory activity was performed by paw edema test induced by carrageenan and 48/80 compounds.The first series of experiments involved[LC]–FIA–IT-MS/MS with 11 separated catechins derivatives until degree of polymerization 3(DP3).The spectra obtained by MALDI-TOF analysis of the AERM presented two homologous series:one based on polymers of m/z 288 Da increments(up to DP12)and another series based on polymers of m/z[288?162]Da increments(up to DP11).In addition to these series of flavan-3-ol,each DP had a subset of masses with a variation of-16 Da(homologous series of afzelechins-m/z 873–3465 Da)and +16 Da(homologous series of gallocatechins-m/z 905–3497 Da).A similar pattern with homologous series of gallocatechins and afzelechins could also be observed for a fifth and a sixth monohexoside series:glucogallocatechins(m/z 779–3371)and glucoafzelechins(m/z 747–3339).The intraperitoneal administration of different doses of AERM(50,150 and 300 lg mL^(-1))have a morphine-like effect and intense anti-inflammatory activity.

关键词： catechins Tannins Morphine-likeeffect Anti-inflammatory Mangroves

Isolation and Purification of a Novel Long-chain Acyl Catechin from Lipophilic Tea Polyphenols

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Chinese Journal of Chemistry 2003年第7期21卷 979-981页

作者：陈平杜琪珍 Department of Chemistry Zhejiang University Hangzhou Zhejiang 310028 China Institute of Food and Biological Engineering Hangzhou University of Commerce Hangzhou Zhejiang 310035 China

Lipophilic tea polyphenols (LTP) was prepared by esterification of green tea polyphenols (GTP) with hexadecanoyl chloride. A novel long-chain acyl catechin was isolated and purified from LTP by high-speed countercurrent chromatography (HSCCC). Its molecular structure was elucidated as epigallocatechin-3-O-gallate-4'-O-hexadecanate by elemental analysis, IR, MS and H-1 NMR spectra.

关键词： lipophilic tea polyphenols green tea polyphenols catechins epigallocatechin-3-0-gallate-4 '-0-hexadecanate high-speed countercurrent chromatography

Determination of catechins in Acer Palmatum Thunb and Acer Ginnala Maxim by HPLC and the Inhibition Effect on FASN

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Determination of Catechins in Acer Palmatum Thunb and Acer G...