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Chondrogenic commitment of human umbilical cord blood and umbilical cord-derived mesenchymal stem cells induced by the supernatant of chondrocytes:A comparison study

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Animal Models and Experimental Medicine 2024年第6期7卷 793-801页

作者： Xingfu Li Zhenhan Deng Wei Lu Department of Orthopedics Shenzhen Second People's Hospital(The First Affiliated Hospital of Shenzhen UniversityHealth Science Center)ShenzhenChina Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging National-Regional Key Technology Engineering Laboratory for Medical UltrasoundSchool of Biomedical EngineeringShenzhen University Medical SchoolShenzhenChina Department of Orthopedics The First Affiliated Hospital of Wenzhou Medical UniversityWenzhouChina

Background:Native cartilage has low capacity for regeneration because it has very few progenitor *** umbilical cord blood-derived mesenchymal stem cells(hUCB-MSCs)and human umbilical cord-derived MSCs(hUC-MSCs)have been employed as promising sources of stem cells for cartilage injury *** of hyaline cartilage from MSCs remains a challenging *** paracrine factors secreted by chondrocytes possess the capability to induce chondrogenesis from ***:The conditioned medium derived from chondrocytes was utilized to induce chondrogenic differentiation of hUCB-MSCs and *** expression levels of collagen type I alpha 1 chain(Col1a1),collagen type II alpha 1 chain(Col2a1),and SRY-box transcription factor 9(SOX9)were assessed through quantitative real-time polymerase chain reaction(qRT-PCR),Western blot(WB),and immunofluorescence(IF)*** elucidate the mechanism of differentiation,the concentration of transforming growth factor-β1(TGF-β1)in the conditioned medium of chondrocytes was quantified using enzyme-l inked immunosorbent assay(ELISA).Meanwhile,the viability of cells was assessed using Cell Counting Kit-8(CCK-8)***:The expression levels of Col2a1 and SOX9 were found to be higher in induced hUC-MSCs compared to those in induced *** conditioned medium of chondrocytes contained TGF-β*** CCK-8 assays revealed that the proliferation rate of hUC-MSCs was significantly higher compared to that of ***:The chondrogenic potential and proliferation capacity of hUC-MSCs surpass those of hUCB-MSCs,thereby establishing hUC-MSCs as a superior source of seed cells for cartilage tissue engineering.

关键词： cartilage diseases chondrogenic differentiation mesenchymal stem cells

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