检索结果-南通市图书馆

Sox9a, not sox9b is required for normal cartilage development in zebrafish

Aquaculture and Fisheries 2021年第3期6卷 254-259页

作者： Qiaohong Lin Yan He Jian-Fang Gui Jie Mei College of Fisheries Huazhong Agricultural UniversityWuhan430070China State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesUniversity of the Chinese Academy of SciencesWuhan430072China

Sox9 is a multifunctional gene and plays crucial roles in vertebrate development including *** teleost,due to the genome duplication event,there are two co-orthologs sox9a and *** this study,CRISPR/Cas9 technology was performed to disrupt the function of either sox9a or *** sox9a mutants(sox9aΔ10 and sox9aΔ67)and sox9b mutants(sox9bΔ11 and sox9bΔ20)lost HMG domain and Q/S domain,however,only sox9a mutant larvae had mis-shaped pectoral fins and lacked the scapulocoracoid ***9b mutant larvae showed normal cartilages similar to wild type *** results suggested that sox9a,not sox9b was required for cartilage development in zebrafish,which was different from the sox9b-mutant phenotype induced by N-ethyl-N-nitrosourea(ENU)treatment,gamma radiation treatment or morpholino *** study confirmed that ancestral sox9 gene functions partitioned between the two paralogs in zebrafish.

关键词： sox9a sox9b cartilage development Zebrafish

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

IRE1α dissociates with BiP and inhibits ER stressmediated apoptosis in cartilage development

IRE1α dissociates with BiP and inhibits ER stressmediated a...

引用

中国遗传学会第九次全国会员代表大会暨学术研讨会

作者： Xiaofeng Han Jinghua Zhou Peng Zhang Rong Jiang Feng-Jin Guo Department of Cell Biology and Genetics Core Facility of Development BiologyChongqing Medical University Laboratory of Stem Cells and Tissue Engineering Chongqing Medical University

Bone morphogenetic protein 2 is known to activate unfolded protein response signaling molecules,including XBP1S,BiP and IREIα.Endoplasmic Reticulum stress is induced in chondrogenesis and activates IRE lαsignal pathway,which is associated with ER stress-mediated ***,the influence on IRElαt and BiP in BMP2-induced chondrocyte differentiation have not yet been elucidated,the molecular mechanism remains unexplored. In this study,we demonstrate that IRE1αinteracts with BiP in unstressed cells and dissociates from BiP in the course of cartilage *** of ER stress-responsive proteins (XBPIS,IRE1α,BiP) was also observed in differentiating ***αinhibition ER stress-mediated apoptosis lies in the process of chondrocyte ***, knockdown of IRE1αexpression by way of the RNAi approach accelerates ER stress-mediated apoptosis in ehondrocyte differentiation induced by BMP2,as revealed by enhanced expression of cleaved caspase3,CHOP and p-JNK1;and this IRE1αinhibition effect on ER stress-mediated apoptosis is required for BiP in chondrogenesis. Collectively,the ER stress sensors were activated during apoptosis in cartilage development, suggesting that selective activation of ER stress signaling was sufficient for induction of apoptosis These findings reveal a novel critical role of IRElαin ER stress-mediated apoptosis and the molecular mechanisms *** results suggest that activation of p-JNKl,caspase3 and CHOP were detected in developing chondrocytes and that specific ER stress signaling leads to naturally occurring apoptosis during cartilage development.

关键词： Endoplasmic reticulum stress Apoptosis UPR IRE1α BiP cartilage development

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Advances in Negative Regulatory Mechanisms of Sox9 Gene Expression during cartilage development

引用

Agricultural Science & Technology 2017年第6期18卷 1038-1041页

作者：刘庆珍吴尽邳晓盟肖慧刘学东郑冬东北林业大学野生动物资源学院黑龙江哈尔滨150040

As the main regulator of cartilage development, Sox9 gene can initiate transcription and expression of various enzymes and protein genes required for car- tilage growth and development. During this process, the expression and function of Sex9 are also regulated by a variety of factors and signaling pathways. More re- search is concerned about the positive regulation. At present, some studies dis- closed that negative regulation of Sox9 expression existed unique mechanisms. This study will discuss and summarize the negative regulatory mechanism of Sox9 gene by microRNA, NF-κB, Wnt, Notch and other factors and signaling pathways, in or- der to provide the basic framework for further investigating the expression and func- tion of Sox9 in cartilage development.

关键词： cartilage development Soxg microRNA NF-κB Wnt Notch

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

IRE1α regulates the PTHrP-IHH feedback loop to orchestrate chondrocyte hypertrophy and cartilage mineralization

引用

Genes & Diseases 2024年第1期11卷 464-478页

作者： Mengtian Fan Nana Geng Xingyue Li Danyang Yin Yuyou Yang Rong Jiang Cheng Chen Naibo Feng Li Liang Xiaoli Li Fengtao Luo Huabing Qi Qiaoyan Tan Yangli Xie Fengjin Guo Laboratory of developmental Biology Department of Cell Biology and GeneticsSchool of Basic Medical ScienceChongqing Medical UniversityChongqing 400016China Laboratory of Stem Cells and Tissue Engineering School of Basic Medical ScienceChongqing Medical UniversityChongqing 400016China Department of Orthopedics The 1st Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Wound Repair and Rehabilitation Medicine State Key Laboratory of TraumaBurns and Combined InjuryTrauma CenterResearch Institute of SurgeryDaping HospitalArmy Medical UniversityChongqing 400042China

cartilage development is controlled by the highly synergistic proliferation and differentiation of growth plate chondrocytes,in which the Indian hedgehog(IHH)and parathyroid hormone-related protein-parathyroid hormone-1 receptor(PTHrP-PTH1R)feedback loop is *** inositol-requiring enzyme 1a/X-box-binding protein-1 spliced(IRE1α/XBP1s)branch of the unfolded protein response(UPR)is essential for normal cartilage ***,the precise role of ER stress effector IRE1α,encoded by endoplasmic reticulum to nucleus signaling 1(ERN1),in skeletal development remains ***,we reported that loss of IRE1α accelerates chondrocyte hypertrophy and promotes endochondral bone ***1 acts as a negative regulator of chondrocyte proliferation and differentiation in postnatal growth *** deficiency interrupted PTHrP/PTH1R and IHH homeostasis leading to impaired chondrocyte hypertrophy and ***1s,produced by p-IRE1α-mediated splicing,binds and up-regulates PTH1R and IHH,which coordinate cartilage ***,ER stress cannot be activated normally in ERN1-deficient *** conclusion,ERN1 deficiency accelerates chondrocyte hypertrophy and cartilage mineralization by impairing the homeostasis of the IHH and PTHrP/PTH1R feedback loop and ER ***1 may have a potential role as a new target for cartilage growth and maturation.

关键词： cartilage development ER stress ERN1 IHH PTHrP/PTH1R

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Activation of the PGC-1α-mediated mitochondrial glutamine metabolism pathway attenuates female offspring osteoarthritis induced by prenatal excessive prednisone

引用

Science China(Life Sciences) 2024年第11期67卷 2382-2397页

作者： Qingxian Li Fan Zhang Yongguo Dai Liang Liu Liaobin Chen Hui Wang Department of Orthopedic Surgery Zhongnan Hospital of Wuhan UniversityWuhan 430071China Joint Disease Research Center of Wuhan University Division of Joint Surgery and Sports MedicineWuhan 430071China Department of Pharmacology School of Basic Medical SciencesWuhan UniversityWuhan 430071China Hubei Provincial Key Laboratory of developmentally Originated Disease Wuhan 430071China

Osteoarthritis is a chronic,age-related joint *** studies have shown that osteoarthritis develops during intrauterine *** is frequently used to treat pregnancies complicated by autoimmune ***,limited research has been conducted on the enduring effects of prednisone use during pregnancy on the *** this study,we investigated the effect of excessive prednisone exposure on cartilage development and susceptibility to osteoarthritis in the *** found that prenatal prednisone exposure(PPE)impaired cartilage extracellular matrix(ECM)synthesis,resulting in poor cartilage pathology in female offspring during the adult period,which was further exacerbated after long-distance running ***,PPE suppressed cartilage development during the intrauterine *** back to the intrauterine period,we found that Pred,rather than prednisone,decreased glutamine metabolic flux,which resulted in increased oxidative stress,and decreased histone acetylation,and expression of cartilage phenotypic ***,PGC-1α-mediated mitochondrial biogenesis,while PPE caused hypermethylation in the promoter region of PGC-1αand decreased its expression in fetal cartilage by activating the glucocorticoid receptor,resulting in a reduction of glutamine flux controlled by mitochondrial ***,overexpression of PGC-1α(either pharmacological or through lentiviral transfection)reversed PPEand Pred-induced cartilage ECM synthesis *** summary,this study demonstrated that PPE causes chondrodysplasia in female offspring and increases their susceptibility to postnatal ***,targeting PGC-1αearly on could be a potential intervention strategy for PPE-induced osteoarthritis susceptibility.

关键词： cartilage development glutamine metabolism mitochondrial biogenesis prenatal prednisone exposure fetal-originated osteoarthritis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：