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欧文氏杆菌CXJZ95-198基因组文库的构建

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中国麻业 2006年第4期28卷 176-181页

作者：张运雄刘正初中国农业科学院麻类研究所湖南长沙410205

采用改良鸟枪法构建了草本纤维精制高效菌种Erwinia carotovora CXJZ95-198的基因组文库,结合透明圈法,筛选到了8个甘露聚糖酶基因阳性克隆,并采用PCR方法对它们进行了分析鉴定,结果表明它们含有同一个甘露聚糖酶基因。

关键词： Erwinia carotovora 基因组文库甘露聚糖酶基因

Use of Polymerase Chain Reaction for Identification of the Pathogen and Management of Potato Soft Rot with Zinc Application

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American Journal of Plant Sciences 2015年第8期6卷 1138-1143页

作者： Ayesha Bibi Muhammad Junaid Musharaf Ahmad Amjad Ali Fazli Wahid Rubina Naz Arif Ullah Amanullah Mahar Shahmir Ali Kalhoro Department of Plant Pathology The University of Agriculture (AUP) Peshawar Pakistan College of Natural Resources and Environment Northwest A&F University Yangling China Department of Soil and Environmental Sciences (AUP) Peshawar Pakistan Department of Botany Kohat University of Science & Technology Kohat Pakistan College of Economics and Management Northwest A&F University Yangling China Faculty of Agriculture Lasbela University of Agriculture Water & Marine Sciences Lasbela Pakistan

Effect of different Zinc doses was investigated against Erwinia carotovora ssp. atroseptica, the potato blackleg/soft rot causing organism, during 2009 and 2010 in Department of Plant Pathology and Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar-Pakistan. Out of 200 tested samples, 21 of them were proved to be Eca. However, these tentative Eca isolates showed some characteristics which were unexpected for Eca. We, therefore, decided to perform Polymerase Chain Reaction using Eca-specific primers, Eca1F and Eca2R for confirm identification. For disease management, at the time of sowing, pots containing 5 kg sterilized soil were applied with Zinc in four different treatments i.e. 8 mg, 10 mg, 12 mg and 14 mg along with one control. Results indicated that 12 mg (4.8 kg Zn ha-1) were better doses in controlling the disease up to 73% and increasing the yield up to 117% as compared to control plants.

关键词： Erwinia carotovora Potato PCR Identification Soft Rot Zinc

Characterization of the Causal Organism of Soft Rot of Tomatoes and Other Vegetables and Evaluation of Its Most Aggressive Isolates

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American Journal of Plant Sciences 2015年第4期6卷 511-517页

作者： Asma Akbar Musharaf Ahmad Azra Neelam Sana Zeb Khan Zahoor Ahmad Department of Plant Pathology The University of Agriculture Peshawar Pakistan Department of Plant Pathology Amir Muhammad Khan Campus Mardan Pakistan Department of Biochemistry The University of Agriculture Peshawar Pakistan Department of Plant Protection The University of Agriculture Peshawar Pakistan Department of Plant Pathology College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province Huazhong Agricultural University Wuhan China Department of Adaptive Research Quetta Balochistan Pakistan

To isolate the causal organism of soft rot of vegetables, diseased samples of potato, tomato, carrot, chilies, and bell pepper, were analyzed in the lab, using nutrient agar (NA) and/or the enrichment host (Bell pepper) technique. Successful isolations were purified by sub-culturing, identified as Erwinia carotovora subsp. carotovora through biochemical tests and their pathogenicity was confirmed through inoculation on green tomato fruits. The isolates were tested for their aggressiveness to find out the most aggressive one in term of producing maximum soft rot on tomatoes. CRD (completely randomized design), with four replication was used and data were analyzed using LSD (least significant test) test. Among the five isolates evaluated for aggressiveness on tomato fruits, chili isolate was found to be the most aggressive followed by tomato and potato isolates producing 22.3 mm, 7.9 mm, and 7.8 mm diameter soft rot lesions, respectively.

关键词： Aggressiveness Bio-Chemical Tests Erwinia carotovora Pathogencity Soft Rot of Tomatoes

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软腐菌识别侵染魔芋根部影响因子的研究

湖北农业科学 2014年第23期53卷 5734-5737页

作者：吴金平矫振彪陈磊夫焦忠久邱正明湖北省农业科学院经济作物研究所武汉430064

魔芋(Amorphophallus Konjac)软腐病频发已成为制约魔芋种植业发展的瓶颈。目前对其病原、发病规律及防治方法等进行了一些研究,加深了对魔芋软腐病的认识,取得了一定进展,但对魔芋软腐病的侵染和发病机制缺乏系统而深入的研究。通过测... 详细信息

魔芋(Amorphophallus Konjac)软腐病频发已成为制约魔芋种植业发展的瓶颈。目前对其病原、发病规律及防治方法等进行了一些研究,加深了对魔芋软腐病的认识,取得了一定进展,但对魔芋软腐病的侵染和发病机制缺乏系统而深入的研究。通过测定软腐菌(Pectobacterium carotovora ***)在魔芋根表吸附量和根部侵入量,研究不同温度、p H、接种浓度、LPS和EPS对软腐菌在魔芋苗根部吸附和侵入过程的影响。结果表明,软腐菌在魔芋苗根部吸附侵入的最适温度条件是30℃、p H 6.0,随接种浓度增加吸附和侵入量增加;菌体LPS是魔芋软腐欧文氏菌对寄主根表的吸附所不可缺少的成分,而EPS预处理对吸附侵入量影响较小。本研究明确不同外界因子(温度、p H、接种浓度)和细菌识别子(EPS和LPS)在软腐菌吸附识别魔芋根际中的作用,从源头上了解软腐菌对魔芋根系的侵染机制,为研究病害循环和制订防治策略提供新的启示,从根本上有效控制魔芋病害的发生,并为魔芋的抗病育种提供新思路。

关键词：魔芋(Amorphophallus Konjae) 软腐菌(Pectobacterium carotovora subsp.carotovora) 吸附侵入

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防治甘薯黑腐病的药剂筛选与使用方法试验

福建农业科技 2004年第2期35卷 41-42页

作者：罗克昌李云平连城县植保站 366200

甘薯细菌性黑腐病是一种发生早、流行快、死株率高、损失惨重的新病害.1997年首次在我县部分乡镇发生流行,到2002年全县发生面积达200hm2,其中100hm2病株率超过20%,1.23 hm2因病害严重而改种.病原菌经福建农林大学植保学院方树民教授鉴... 详细信息

甘薯细菌性黑腐病是一种发生早、流行快、死株率高、损失惨重的新病害.1997年首次在我县部分乡镇发生流行,到2002年全县发生面积达200hm2,其中100hm2病株率超过20%,1.23 hm2因病害严重而改种.病原菌经福建农林大学植保学院方树民教授鉴定为Erwinia carotovora sp..为遏制病害流行,开展了防治技术和药剂筛选试验.

关键词：甘薯黑腐病药剂筛选使用方法 Erwinia carotovora sp.