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Installing the neurospora carotenoid pathway in plants enables cytosolic formation of provitamin A and its sequestration in lipid droplets

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Molecular Plant 2023年第6期16卷 1066-1081页

作者： Xiongjie Zheng Yasha Zhang Aparna Balakrishna Kit Xi Liew Hendrik N.J.Kuijer Ting Ting Xiao Ikram Blilou Salim Al-Babili The BioActives Lab Center for Desert Agriculture(CDA)Biological and Environmental Science and Engineering(BESE)King Abdullah University of Science and Technology(KAUST)Thuwal 23955-6900Saudi Arabia The Laboratory of Plant Cell and Developmental Biology(LPCDB) Center for Desert Agriculture(CDA)Biological and Environmental Science and Engineering(BESE)King Abdullah University of Science and Technology(KAUST)Thuwal 23955-6900Saudi Arabia Present address:College of Plant Science and Technology Bejing Key Laboratory for Agricultural Application and New TechniqueBeijing University of AgricultureBeijing 102206China

Vitamin A deficiency remains a severe global health issue,which creates a need to biofortify crops with provitamin A carotenoids(PACs).Expanding plant cell capacity for synthesis and storing of PACs outside the plastids is a promising biofortification strategy that has been little ***,we engineered PAC formation and sequestration in the cytosol of Nicotiana benthamiana leaves,Arabidopsis seeds,and citrus callus cells,using a fungal(Neurospora crassa)carotenoid pathway that consists of only three enzymes converting C5 isopentenyl building blocks formed from mevalonic acid into PACs,including β-*** strategy led to the accumulation of significant amounts of phytoene and γ-and β-carotene,in addition to fungal,health-promoting carotenes with 13 conjugated double bonds,such as the PAC torulene,in the *** the isopentenyl diphosphate pool by adding a truncated Arabidopsis hydroxymethylglutaryl-coenzyme A reductase substantially increased cytosolic carotene *** carotenes accumulate in cytosolic lipid droplets(CLDs),which represent a novel sequestering sink for storing these pigments in plant ***,β-carotene accumulated in the cytosol of citrus callus cells was more light stable compared to compared with plastidialβ-***,engineering cytosolic carotene formation increased the number of large-sized CLDs and the levels of β-apocarotenoids,including retinal,the aldehyde corresponding to vitamin ***,our study opens up the possibility of exploiting the high-flux mevalonic acid pathway for PAC biosynthesis and enhancing carotenoid sink capacity in green and non-green plant tissues,especially in lipid-storing seeds,and thus paves the way for further optimization of carotenoid biofortification in crops.

关键词： Neurospora crassa carotenoid biosynthesis provitamin A metabolic engineering synthetic metabolism cytosolic lipid droplets carotenoid sequestration carotenoid stability

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